Flower buds of capers were processed by some common methods as pickling,
salting, and other uncommon like freezing with and without blanching and canning. The
results showed that there was no microbiological growth in all methods since the fourth
month of storage. Best growth for lactobacillus bacteria was in 5% brine (8.2 × 106) and
then in 8% brine (1.2 × 106)CFU.g-1 . while there wasn’t any growth of this bacteria by
using other methods like using high concentration of salt 23%. On other hand, the results
showed that this bacteria existed in small account (1.4 × 102) and (1.1 × 102) CFU.g-1
after a week in brine 16% and the freezed sample respectively. There was no statistical
difference between the two storage solutions as there wasn̓ t any microbiological growth in
both of them through 8 months.
Samples of Capparis spinosa plant were collected from 6 sites in Aleppo and Lattakia
provinces. The genetic variations were studied using the AFLP technique in order to determine the
genotypes of the studied types using 3 primers, which showed ampl
ification. Statistical analyses
were conducted using the dissimilarity coefficient and genetic diversity coefficient. The A.C.P. and
the statistical -F were calculated, and the similarity dendrogram was constructed. The results
showed the following:
The presence of a certain number of specific alleles (descriminates) for each province.
The presence of genetic and reproductive isolation deterrent to gene flow between the two
provinces.
The heterozygoty average was a little higher in Lattakia (0.486) than in Aleppo (0.481). The
mean of genetic diversity coefficient of primers and individuals was a little higher in Aleppo
(0.677) than in Lattakia (0.653). The population mean was very close: (0.759) in Aleppo and
(0.760) in Lattakia. This may be explained on the basis of silent and specific alleles due to deletion
mutations, different selective effects as well as the reproduction system in the two cities.
The greatest genetic distance in Aleppo was (0.381) noted between (Al Dahea and Al Shekh
saaed), and in Lattakia (0.38) noted between (Al Amroniah and Jabla). However, the greatest
genetic similarity in Aleppo was (0.637) noted between (Al Shekh Saaed and Turkman Bareh), and
in Lattakia (0.675) noted between (Wata Deirzenon and Jabla). The variation between these values
was graduated.
The study of statistical –F showed the effect of the similarity factor in some populations.
This was more significant in Aleppo than in Lattakia, which indicates that the reproduction system
is more closed in Aleppo, and this refers as well to inbreeding or self pollination which showed
high genetic variations in these populations when compared with Lattakia.
The results of this study helped in determining primers that can be used as molecular
markers in a breeding program for Capparis spinosa as a medicinal plant. This technique showed
high efficiency in studying the similarity relationships between these two cities.
In the present study¸ twelve parameters of Capparis spinosa were studied which are: plant length¸
number of main branches¸ number of leaf doubles, leaf length, leaf width, leaf area¸ number of budes,
number of flowers, number of fruits, weight of t
he fruits, number of seeds, weight of seeds. Statistical
analysis have been done using mean¸ variation¸ A.F.C.