هدف البحث إلى تقييم التنوع الوراثي و دراسة درجة القرابة الوراثية بين 16 طـرازاً وراثيـاً مـن
الشعير المزروع (L vulgare Hordeum) (أصناف معتمدة، سلالات جديدة) باستخدام مؤشرات SSR .
استخدم 32 زوجاً من بادئات المقاطع القصيرة المتكررة SSR ،و تبين أن 5 منها لم تعط تعددية شـكلية،
بينما استخدم الـ 27 زوجاً المتبقية في تقييم التنوع الوراثي، و أنتجت ما مجموعه 126 قريناً أي بمعدل
66.4 قريناً لكل موقع وراثي. كما تراوحت عدد القرائن لكل موقع وراثي بين قرينين و 11 قريناً. أما قيمة
معامل التعددية الشكلية (PIC) فقد تراوحت بين 21.0 و 88.0 .و كانت أكثر البادئات قدرة على التمييـز
بين الطرز الوراثية هيscssr07970 ، scssr10148 ،Bmag0125 ،scssr03907 .أما قيمة التنـوع
المورثي فقد راوحت بين 23.0 و 89.0 .و قد توزعت الطرز المدروسة في شجرة القرابة الوراثية التـي
أنشئت اعتماداً على نتائج الـ SSR إلى ثلاثة عناقيد. وجدت أعلى درجة قرابة وراثية بين الـسلالة39
و السلالة 38 و قد أظهرت مؤشرات SSR قدرة عالية على التمييز بين الطـرز الوراثيـة المدروسـة مـن
الشعير.
The research aimed to evaluate the genetic diversity and the genetic
relationship between 16 Hordeum vulgare genotypes (variaties and new lines),
using SSR markers. 32 primer pairs were used in the analysis, five of them
amplified primers monomorphic alleles, while the remaining primers detected
different alleles. A total of 126 polymorphic alleles were revealed by the 27
primer pairs. The number of different alleles detected on alocus ranged from 2
to 11with a mean of 4.66 alleles per locus. The polymorphic information content
(PIC) values ranged from 0.21 to 0.88 with a mean value of 0.49, and the gene
diversity ranged from 0.23 to 0.89 with a mean value of 0.53.The dendrogram
was established using SSR data clustered into three major groups. The smallest
genetic relationship was observed between line39 and line38 . The results proved
the efficiency of SSR markers in the estimation of genetic diversity and in the
clustering of the barley genotypes in Syria.
References used
Abebe, TD. 2010. Genetic diversity and population differentiation analysis of ethiopian barley (Hordeum vulgare L) landraces using morphological traits and SSR markers. PhD thesis. Institut für Nutzpflanzenwissenschaften und Ressourcenschutz
Baum, M.. S. Grando and S. Ceccarelli. 2004. Localization of quantitative trait loci for dryland characters in barley by linkage mapping. Crop Science Society of America and American Society of Agronomy, 677 S. Segoe Rd., Madison, WI 53711, USA. Challenges and Strategies for Dryland Agriculture. CSSA Special Publication no. 32
Becker, J. and M. Heun. 1995. Barley microsatellites allele variation and mapping, Plant Mol. Biol. 27:835-845
A half diallel set of crosses among six highly diverse barley (Hordeum
vulgare L.) genotypes were evaluated at Karahta station of field crops research
(GCSAR) Damascus Governorate, during 2010-2011 and 2011-2012 seasons to
study heterosis and comb
This study present first estimates of segregation distortion
as revealed by comparison of segregation ratios in DH lines and sexually
produced F٢ lines in barley using the co-dominant microsatellite markers
(STMS).
This cultivation was carried out in the farm of the Faculty of Agriculture at Damascus University during the 2013-2014 agricultural season to study the effect of seeding rate per unit area and planting dates on yield and its components for three barley genotypes under rain-fed conditions.
The research was executed in Biotechnology Lab (Faculty of
Agriculture – Damascus University), during the season 2016.
Sixteen genotypes of Sunflower Oil were planted to study the
genetic diversity among these genotypes and to determine the
degree of genetic Agreement using the technique SSR (Simple
Sequence Repeats).
The research was carried out in the biotechnology Laboratory at the
Faculty of Agriculture, Damascus University, in cooperation with the General
Commission For Scientific Agricultural Research (GCSAR), during the year
2012. Eleven genotypes of cul