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The genetic diversity in some syrian Hordeum vulgare L. genotypes using SSR markers

التنوع الوراثي لطرز من الشعير المزروع .L vulgare Hordeum في سورية باستخدام واسمات SSR

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 Publication date 2014
and research's language is العربية
 Created by Shamra Editor




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The research aimed to evaluate the genetic diversity and the genetic relationship between 16 Hordeum vulgare genotypes (variaties and new lines), using SSR markers. 32 primer pairs were used in the analysis, five of them amplified primers monomorphic alleles, while the remaining primers detected different alleles. A total of 126 polymorphic alleles were revealed by the 27 primer pairs. The number of different alleles detected on alocus ranged from 2 to 11with a mean of 4.66 alleles per locus. The polymorphic information content (PIC) values ranged from 0.21 to 0.88 with a mean value of 0.49, and the gene diversity ranged from 0.23 to 0.89 with a mean value of 0.53.The dendrogram was established using SSR data clustered into three major groups. The smallest genetic relationship was observed between line39 and line38 . The results proved the efficiency of SSR markers in the estimation of genetic diversity and in the clustering of the barley genotypes in Syria.

References used
Abebe, TD. 2010. Genetic diversity and population differentiation analysis of ethiopian barley (Hordeum vulgare L) landraces using morphological traits and SSR markers. PhD thesis. Institut für Nutzpflanzenwissenschaften und Ressourcenschutz
Baum, M.. S. Grando and S. Ceccarelli. 2004. Localization of quantitative trait loci for dryland characters in barley by linkage mapping. Crop Science Society of America and American Society of Agronomy, 677 S. Segoe Rd., Madison, WI 53711, USA. Challenges and Strategies for Dryland Agriculture. CSSA Special Publication no. 32
Becker, J. and M. Heun. 1995. Barley microsatellites allele variation and mapping, Plant Mol. Biol. 27:835-845
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