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Register a new userAnatomical Study of Caluus Stages Intiation and its Development to Embryoiods in Date Palm Phoenix dactylifera L. Cultured In Vitro
دراسة تشريحية لمراحل نشوء نسيج الكالس و تطوره إلى أجنة خضرية و نباتات كاملة من نخلة التمر (Phoenix dactylifera L المزروعة خارج الجسم الحي)
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Damascus University ورقة بحثية
Publication date
2003
fields
Biology
and research's language is
العربية
Authors
عبد المنعم حسين علي( باحث )
Created by
Shamra Editor
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أجريت دراسة تشريحية زمنية لمراحل نشوء نسيج الكالس الأولي و تطوره إلى كالس جنيني و أجنة خضرية و ذلك ابتداء من الأسبوع الثاني من زراعة قطع البراعم الطرفية حتى نهاية الشهر العاشر في وسط غذائي MS يحتوي على الأوكسين نوع D ٢،٤ بتركيز ١٠٠ ملغم/لتر مع مسحوق الفحم المنشط و في الظلام. و قد أظهرت الدراسة أن الخلايا المرستيمية النشطة المنتشرة بصورة غير متساوية في النسيج الأصلي و المتمركزة في حدبة البرعم الطرفي و في النهايات المحدبة من الوريقات البدائية في البرعم هي المنطلق الأساسي لنمو نسيج الكالس الأولي. ظهرت الأجنة الخضرية أول مرة بشكل مجاميع خلوية مرستيمية منفصلة عن بعضها بجدران سميكة نسبيًا بعد مرور خمسة أشهر من الزراعة على الوسط الغذائي عالي الأوكسين. أما الأشهر المتقدمة من ٦ حتى نهاية ١٠ اشهر فبدأت الأجنة الخضرية الأولية بالتقدم إلي أجنة متعددة الخلايا، كما لوحظ ميل الأجنة إلى التجزؤ و الانفصال و التبرعم لإنتاج أعداد أخرى من الأجنة.
Stages of initial callus and embryiods of date palm Phoenix dactylifera L. were histological and chronological studied begging from the ٢nd weeks of culturing segment of shoot tip to ten months in MS medium containing ١٠٠mg/l ٢،٤-D with activated charcoal in the dark. The study reveled that the active meristematic cells were unequally distributed in the explant and concentrated in the shoot apical dome and leaf margin. These cells are the bases from which initial callus started its growth. Somatic proembryo appeared for the first time as a meristematic cells groups separated from each other by relatively thick walls after five months of culturing in continuos culture in the same medium .In progress months (٦-١٠) the small simple proembryos continued into advanced multicell embryos and underwent polarization. Also the embryos were observed with attendance for division and separation into smaller or budded to produce numerous other.
Artificial intelligence review:
Research summary
تتناول هذه الدراسة التشريحية مراحل تكوين الكالوس وتطوره إلى الأجنة في نخيل التمر Phoenix dactylifera L. المزروع في المختبر. تم دراسة هذه المراحل من خلال الفحص النسيجي والزمني بدءًا من زراعة جزء من قمة البرعم لمدة عشرة أشهر في وسط MS يحتوي على 2,4-D مع الفحم النشط في الظلام. كشفت الدراسة أن الخلايا الميرستيمية النشطة كانت موزعة بشكل غير متساوٍ في العينة ومركزة في قمة البرعم القمي وحواف الأوراق. ظهرت الأجنة الجسدية لأول مرة كمجموعات من الخلايا الميرستيمية مفصولة بجدران سميكة نسبيًا بعد خمسة أشهر من الزراعة. مع مرور الأشهر، استمرت الأجنة البسيطة الصغيرة في التطور إلى أجنة متعددة الخلايا وخضعت للاستقطاب. كما لوحظت الأجنة وهي تنقسم وتنفصل لتنتج العديد من الأجنة الأخرى.
Critical review
دراسة نقدية: تقدم هذه الدراسة رؤى قيمة حول تكوين الكالوس وتطوره إلى الأجنة في نخيل التمر، مما يساهم في فهم أفضل لعملية التكاثر الجسدي في هذه النباتات. ومع ذلك، هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الممكن توفير تفاصيل أكثر دقة حول الظروف البيئية المحددة التي تم فيها إجراء التجارب، مثل درجة الحرارة والرطوبة. ثانيًا، كان من الممكن أن تكون الدراسة أكثر شمولاً إذا تم تضمين مقارنة مع أنواع أخرى من النباتات أو استخدام تقنيات مختلفة لتحليل النتائج. أخيرًا، قد يكون من المفيد تضمين توصيات تطبيقية لكيفية استخدام هذه النتائج في تحسين زراعة نخيل التمر على نطاق واسع.
Questions related to the research
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ما هي الخلايا الميرستيمية وأين تتركز في العينة؟
الخلايا الميرستيمية هي خلايا نشطة صغيرة الحجم وكثيفة السيتوبلازم وكبيرة النواة، وتتركز في قمة البرعم القمي وحواف الأوراق.
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متى ظهرت الأجنة الجسدية لأول مرة في الدراسة؟
ظهرت الأجنة الجسدية لأول مرة بعد خمسة أشهر من الزراعة في نفس الوسط.
-
ما هي الظروف التي تم فيها زراعة أجزاء البرعم في هذه الدراسة؟
تم زراعة أجزاء البرعم في وسط MS يحتوي على 2,4-D مع الفحم النشط في الظلام.
-
كيف تطورت الأجنة الجسدية مع مرور الوقت في الدراسة؟
استمرت الأجنة البسيطة الصغيرة في التطور إلى أجنة متعددة الخلايا وخضعت للاستقطاب، ومع مرور الأشهر، زاد عدد الأجنة وانقسمت لتنتج العديد من الأجنة الأخرى.
References used
Al-Musawi,A.H.A (٢٠٠١). In vitro production of somatic embryo from different callus stages cultured on high auxin medium in date palm Phoenix dactylifera L. B.D.P.R.J ١(١):٧-١٨.(In Arabic)
Ai-Utbi ,S.D (١٩٩٨). Study of vegetative propagation of date palm Phoenix dactylifera L. invitro and the effect of the addition of its flowers and seeds on growth at different morphogenic stages.Ph. D. Thesis. Biology Dept.Science college .Basrah Univ.Basrah ,Iraq. ١١١pp.(In Arabic
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