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Bone healing is a complicated biological mechanism that is affected by many hormonal, nutritional, and hemal factors. The term Biomechanical theory of bone healing is a concept that is created by us and is used for the first time to identify and stud y the influence of mechanical rules on the bone tissue so we can study and imitate its mechanical response depending on the rules of balance and movement. We ensure that bone healing is just adaptation to auto bone forming mechanism in the surrounding mechanical site. The term is more comprehensive and accurate than the bio-compression term which was used before; and all of those are confirming the criteria to evaluate the efficacy of fixators used in orthopedic surgery.
This Research was conducted in the laboratory of tissue culture affiliated to the National Commission of Biotechnology (NCBT) during the period between 2011 -2012 for induction of callus from mature embryos for three local Genotypes of grain Sorgh um, regeneration of the plant from callus, rooting plantlets and acclimatization in order to get a plant capable to grow in greenhouse. The best concentration for sterilizing the plant and entering it in use was 5% NaOCL for 20 minutes. The addition of 2 mg/l of 2,4-D (2,4- dichlorophenoxyacetic acid) to MS medium caused an increase in the ratio of callus induction and embryogenic callus.
Stages of initial callus and embryiods of date palm Phoenix dactylifera L. were histological and chronological studied begging from the ٢nd weeks of culturing segment of shoot tip to ten months in MS medium containing ١٠٠mg/l ٢،٤-D with activated charcoal in the dark. The study reveled that the active meristematic cells were unequally distributed in the explant and concentrated in the shoot apical dome and leaf margin. These cells are the bases from which initial callus started its growth. Somatic proembryo appeared for the first time as a meristematic cells groups separated from each other by relatively thick walls after five months of culturing in continuos culture in the same medium .In progress months (٦-١٠) the small simple proembryos continued into advanced multicell embryos and underwent polarization. Also the embryos were observed with attendance for division and separation into smaller or budded to produce numerous other.
This investigation was carried out at Tissue Culture Laboratory, Department of Horticulture, University of Mosul, Iraq during 2012-2014) on Catharanthus roseus Variety; Nirvana Pink Blush, which is one of the important medicinal plant, containing a group of alkaloids. Parts of leaves, nodes, and internodes were cultured on MS medium supplemented with NAA for callus initiation, data were taken after 28-day results refer that highest weight of callus; 0.612g, 0.639g ,0.835g were obtained from cultivated parts of leaves, nodes and internodes on MS medium supplemented with 1.0mg/l NAA respectively. Callus was initiated and cultured using parts of leaves, nodes and internodes on MS medium supplemented with BA and 1.0 mg/l of NAA, after 50 days.
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