The objective of this research is to propagate Mentha puligium in
vitro, shoot tips and nodal explant were planted with 0.5-1 cm length
on MS (Murashige's and Skoog) medium with various concentrations
(0.5-1-2-3-4) mg/L of cytokinin BAP.
This investigation was carried out at Tissue Culture Laboratory, Department
of Horticulture, University of Mosul, Iraq during 2012-2014) on Catharanthus
roseus Variety; Nirvana Pink Blush, which is one of the important medicinal
plant, containing
a group of alkaloids. Parts of leaves, nodes, and internodes were
cultured on MS medium supplemented with NAA for callus initiation, data were
taken after 28-day results refer that highest weight of callus; 0.612g, 0.639g ,0.835g
were obtained from cultivated parts of leaves, nodes and internodes on MS medium
supplemented with 1.0mg/l NAA respectively. Callus was initiated and cultured
using parts of leaves, nodes and internodes on MS medium supplemented with BA
and 1.0 mg/l of NAA, after 50 days.
