This research was conducted at the Laboratory of Tissue Culture, Faculty of Agriculture, Tishreen University. Because of the importance of the food and economic of chickpea, two Winter chickpea cultivars (Ghab 4, Ghab 5) and two Vernal chickpea culti
vars were used (ILC263, ILC1929) and planted in pots until flowering, so the floral buds were picked before opened and washed with distilled water and alcohol three times. Anthers were separated and treated at preliminary temperatures (4 Cْ for 48 hours, and 35 Cْ for 12 hours), then it washed with a solution of hypochlorite of sodium( Naocl 2%) for a period of 15 minute, and washed with distilled sterilized water ,then where planted on the environment Murashige and Skoog ( MS) equipped with 1, 3 and 5 mg/L of auxin 2,4, D, as well as 0.1, 0.2 and 0.3 mg /L of Cytokinin Benzyl Amino Purine( BAP) individually and with interaction between them and incubated under conditions of 27 Cْ and 75% of the humidity and the intensity of 1500 Lux of light for 16 hours. The main objective of research was to study the effect of both preliminary heating treatment , quality and concentration of used hormone on producing callus from used chickpea cultivars anthers. Results showed difference in cultivars response in the treatment of both temperature and single hormone, so Ghab 5 cultivar was the most responsive to the formation of callus, while the results indicated that the highest percentage of the formation of callus was 40% when treated at 4 Cْ of Ghab 4 and Ghab 5 cultivars, while ILC263 cultivar showed less response in all the individual treatments for growth regulators.
As well as results showed that the significant and catalyst role for using of hormones together with heat treatment, where each of Ghab 5, Ghab 4 and ILC1929 cultivars were the superiority, by the arrival of the percentage of 80% and 60%, respectively.
The aim of this investigation is studying effect of heat treatment and polyethylene packaging method in storability and quality of lemon fruits' Monachello and Enterdonato'. The experience was carried out in cold store of agriculture college in /Dama
scus university/, lemon fruits were stored at 10°c and 85% humidity for 180 days. laboratory experiences was carried out in laboratory of department of horticultural science in the same college during 2010-2011&2011-2012. Part of fruits were packaged in polyethylene bags with 30 microns, the other part of fruits dipped with hot water (62°c for 3 seconds)+ fungicide then were packed in polyethylene packs in addition to the control which untreated and unpacked.
The obtained results indicated the significant effect of dipping and packaging in perforated and unperforated bags treatments in maintain fruits quality of 'Monachello and Enterdonato' until the end of storage. maintaining of (32.89 and 39.06%( juice rate of Monachello respectively, and(11.05 and10.01%) of Enterdonato respectively. (35.27 and 36.57 mg/100ml (vitamin C of Monachello and (18 and11.07mg/100 ml( of Enterdonato, respectively.
On the other hand, the present investigation showed the positive effect of packaging treatment with 30 microns packs by reducing the weight loss for two sorts which was (51.94 and 68.83%) in dipping and packaging in perforated bags treatment respectively, and the important role to stop fruit decadence, and conserve their marketing specification until end of storage.
