Stages of initial callus and embryiods of date palm Phoenix dactylifera L.
were histological and chronological studied begging from the ٢nd weeks of
culturing segment of shoot tip to ten months in MS medium containing ١٠٠mg/l
٢،٤-D with activated
charcoal in the dark. The study reveled that the active
meristematic cells were unequally distributed in the explant and concentrated
in the shoot apical dome and leaf margin. These cells are the bases from which
initial callus started its growth. Somatic proembryo appeared for the first time
as a meristematic cells groups separated from each other by relatively thick
walls after five months of culturing in continuos culture in the same medium .In
progress months (٦-١٠) the small simple proembryos continued into advanced
multicell embryos and underwent polarization. Also the embryos were
observed with attendance for division and separation into smaller or budded to
produce numerous other.
This investigation was carried out at Tissue Culture Laboratory, Department
of Horticulture, University of Mosul, Iraq during 2012-2014) on Catharanthus
roseus Variety; Nirvana Pink Blush, which is one of the important medicinal
plant, containing
a group of alkaloids. Parts of leaves, nodes, and internodes were
cultured on MS medium supplemented with NAA for callus initiation, data were
taken after 28-day results refer that highest weight of callus; 0.612g, 0.639g ,0.835g
were obtained from cultivated parts of leaves, nodes and internodes on MS medium
supplemented with 1.0mg/l NAA respectively. Callus was initiated and cultured
using parts of leaves, nodes and internodes on MS medium supplemented with BA
and 1.0 mg/l of NAA, after 50 days.
