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Register a new userIsolation of Enterobacter sakazakii from Some Spices
عزل بكتيريا الأمعائية الساكازاكية من بعض أنواع التوابل
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Damascus University ورقة بحثية
Publication date
2011
fields
Biology
and research's language is
العربية
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Shamra Editor
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تُعد بكتيريا sakazakii Enterobacter من العوامل الممرضة الانتهازية المسببة لإصابات مميتـة عند الأطفال و كبار السن و البالغين منقوصي المناعة، و هي بكتيريا عصوية الشكل سالبة الغرام لا هوائية اختيارية تنتمي إلى فصيلة Enterobacteriaceae و جنس Enterobacter .و مع أن الموطن الطبيعي لهذه البكتيريا غير محدد بدقة إلا أن وجودها بنسبة كبيرة في التوابل و الأعشاب قد يشير إلى أن النباتات تمثل الخازن الطبيعي لها.
Enterobacter sakazakii is considered an opportunistic pathogen that has been associated with severe lethal infections especially in neonates, elderly, and Immunocompromised adults. E. sakazakii is a Gram negative, facultative anaerobes rod-shaped bacterium. It belongs to the family Enterobacteriaceae and genus Enterobacter. Although we don’t know the natural habitat of this bacteria we find that it exists in high rate in herbs and spices which indicates that plant may be this natural habitat.
References used
Farmer J. J., III, Asbury M. A., Hickman F. W., Brenner D. J. (1980). The Enterobacteriaceae Study Group (USA): Enterobacter sakazakii: a new species of "Enterobacteriaceae" isolated from clinical specimens. Int. J. Syst. Bacteriol. 30:569-584
Iversen C., Waddington M., On S. L., Forsythe S. (2004). Identification and phylogeny of Enterobacter sakazakii relative to Enterobacter and Citrobacter species. J. Clin. Microbiol. 42:5368-5370
Iversen C., Waddington M., Farmer J. J. III, Forsythe S. (2006). The biochemical differentiation of Enterobacter sakazakii genotypes. BMC Microbiol. 6:94
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This research was conducted at the Faculty of Agriculture, Department of
Food Science laboratories to isolate lactic acid bacteria from some Syrian milk
products (milk, yogurt, cheese) and determine some morphological,
physiological characteristic and the ability of these isolates to ferment sugars.
Spices are becoming increasingly important world-wide due to their effectiveness as natural antibiotics, the thing that makes the world nowadays tend to use spices instead of chemical drugs. However, this doesn’t mean they are free of some dangerous
pathogens such as Klebsiella spp. which can lead to a wide range of illnesses, notably pneumonia, urinary tract infections (UTIs), and bacteremia. Although we don’t know the natural habitat of this bacterium we find that it thrives in herbs and spices, concluding thus that plants may be its natural habitat.
The primary isolation of this bacterium was based first on its growth on general media and then isolation on selective media. Colonies of the microorganisms were counted on different media then Klebsiella was isolated from a selective media. The isolated colonies of Klebsiella were circular, dome-shaped, 3-4 mm in diameter, with mucoid sticky edges, and surrounded by capsule. After this we chose the typical colony and identified it using Polymerase chain reaction PCR and biochemical tests. Our results showed that 32% of examined specimen contained Klebsiella spp.
This study has been conducted to identify the levels of heavy
metals (lead, cadmium, nickel, cobalt, copper, iron, manganese, and
zinc) in the following spices: Nutmeg, turmeric, cinnamon, ginger,
cardamom, nigella sativa, black pepper, cumin, coriander, curry,
and mixed spices.
This study was conducted at the laboratory of Food Science Department,
Agriculture College, Damascus University to isolate and characterize of
Lactobacilli bacteria from some Syrian foods and detection the effectiveness of
anti-pathogenic bacteria
l growth. Lactic acid bacteria from different dairy
products, pickles and dried plant samples were isolated and identified
according to common characteristics. Results showed that twenty-three isolates
were rods while only 2 of the them were coccid. These isolates were tested for
their antimicrobial effect against pathogenic bacteria for both gram positive
bacteria (Staphylococcus aureus) and gram negative bacteria (Klebsiella
pneumonia and Escherichia coli). Fifteen isolates demonstrated antimicrobial
effect against the three tested bacteria, of which CP50 and CP46 isolates
showed the best antimicrobial effect. Results of the standard physiological and
biochemical tests identified fourteen isolates as Lactobacillus plantarum and one
isolate as Lactobacillus fermentum.
This study were conducted on 450 samples of raw chicken meat
( 150 samples of thighs- 150 samples of breasts -150 samples of
wings ) were collected from retail marketin Hama city,to detect
contamination of staphylococcus.
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