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Isolation and characterization of lactic acid bacteria from some Syrian diary products

عزل و توصيف أنواع بكتيريا حمض اللبن من بعض منتجات الألبان السورية

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 Publication date 2014
and research's language is العربية
 Created by Shamra Editor




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This research was conducted at the Faculty of Agriculture, Department of Food Science laboratories to isolate lactic acid bacteria from some Syrian milk products (milk, yogurt, cheese) and determine some morphological, physiological characteristic and the ability of these isolates to ferment sugars.



References used
Abdullah, A. S. and M.M. Osman. 2010. Isolation and Identification of Lactic Acid Bacteria from Raw Cow Milk, White Cheese and Rob in Sudan.Pakistan Journal of Nutrition .9 (12): 1203-1206. Issn 1680-5194
Ali, A. A. 2011. Isolation and Identification of Lactic Acid Bacteria from Raw Cow Milk in Khartoum State, Sudan. International Journal Dairy Science. 6(1): 66–71
Axelsson, L. 2004. Lactic Acid Bacteria: Classification and physiology. In: Lactic Acid Bacteria, Microbiological and Functional Aspects. Salminen, A.V. and A. O. Wright (Eds.) ouwehand. Marcel Dekker, New York, pp: 1- 66
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This research was conducted in the Agriculture Faculty- Food Science Department, Damascus University, for detecting Lactic acid bacteria isolated from Syrian dairy products (white fresh cheese-Leben), Samples were collected from August, 2004 to Oc tober, 2005 from different areas in Syria. Analysis was done for 245 bacterial isolates from 40 samples of white fresh cheese and 58 samples of yogurt (curd). Results showed that the genus Enterococcus was dominant with a percentage of 47.52%; Pediococcus had a percentage of 26.3 %; while Lactococcus had a percentage of 15.38% with only 3.2% for Lactobacillus.
This study was conducted at the laboratory of Food Science Department, Agriculture College, Damascus University to isolate and characterize of Lactobacilli bacteria from some Syrian foods and detection the effectiveness of anti-pathogenic bacteria l growth. Lactic acid bacteria from different dairy products, pickles and dried plant samples were isolated and identified according to common characteristics. Results showed that twenty-three isolates were rods while only 2 of the them were coccid. These isolates were tested for their antimicrobial effect against pathogenic bacteria for both gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Klebsiella pneumonia and Escherichia coli). Fifteen isolates demonstrated antimicrobial effect against the three tested bacteria, of which CP50 and CP46 isolates showed the best antimicrobial effect. Results of the standard physiological and biochemical tests identified fourteen isolates as Lactobacillus plantarum and one isolate as Lactobacillus fermentum.
Fifty eight isolates of lactic acid bacteria were obtained from different local fermented foods. Isolates were identified using microscopic, cultural and biochemical tests using API strips. Results showed that 44.83% of the isolates were rod shape d and 55.17% belonged to cocci bacteria.
Total of 33 isolates of lactic acid bacteria from different samples (Dairy products, Pickles), were isolated and identified according to the common characteristics of lactic acid bacteria. Twinty-five isolates were rods and 9 were cocci.
أجري هذا البحث في مخابر كلية الزراعة بجامعة الفرات قسم علوم الأغذية وفي مخابر هيئة الطاقة الذرية بدمشق وقد هدف إلى عزل وتعريف بعض سلالات بكتريا حمض اللبن من الحليب الخام للنوق والماعز الشامي بالإضافة إلى دراسة بعض الخصائص الكيميائية والتصنيعية للسلالات المعزولة وذلك في عامي ( 2010و 2011)
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