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Isolation and characterization of Lactobacilli bacteria from some Syrian foods and detection the effectiveness of anti-pathogenic bacterial growth

عزل بكتيريا حمض اللبن العصوية من بعض الأغذية السورية و تصنيفها و التحري عن فعاليتها المضادة لنمو البكتيريا الممرضة

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 Publication date 2012
  fields Food Sciences
and research's language is العربية
 Created by Shamra Editor




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This study was conducted at the laboratory of Food Science Department, Agriculture College, Damascus University to isolate and characterize of Lactobacilli bacteria from some Syrian foods and detection the effectiveness of anti-pathogenic bacterial growth. Lactic acid bacteria from different dairy products, pickles and dried plant samples were isolated and identified according to common characteristics. Results showed that twenty-three isolates were rods while only 2 of the them were coccid. These isolates were tested for their antimicrobial effect against pathogenic bacteria for both gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Klebsiella pneumonia and Escherichia coli). Fifteen isolates demonstrated antimicrobial effect against the three tested bacteria, of which CP50 and CP46 isolates showed the best antimicrobial effect. Results of the standard physiological and biochemical tests identified fourteen isolates as Lactobacillus plantarum and one isolate as Lactobacillus fermentum.

References used
Adenike, A. O., A. Mopelola and J. Adeleye 2007. In vitro antimicrobial characteristics of bacteriocinproducing Lactobacillus strains from Nigerian indigenous fermented foods. Afri. J. Biotechno., 6 (4):445-453
Ananou, R. C., S. Ananou, M. Maqueda, M. Martínez-Bueno and E. Valdivia. 2007. Bio - preservation, an ecological approach to improve the safety and shelf-life of foods. Communicating Current Research and Educational Topics and Trends in Applied Microbiology, Pp: 475-486
Atrih, A., N. Rekhif, J. P. Milliere and G. Lefebvre. 1993 Detection and characterization of a bacteriocin produced by Lactobacillus plantarum C19. Can. J. Microbiol., 39, 1173–1179
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Total of 33 isolates of lactic acid bacteria from different samples (Dairy products, Pickles), were isolated and identified according to the common characteristics of lactic acid bacteria. Twinty-five isolates were rods and 9 were cocci.
This research was conducted at the Faculty of Agriculture, Department of Food Science laboratories to isolate lactic acid bacteria from some Syrian milk products (milk, yogurt, cheese) and determine some morphological, physiological characteristic and the ability of these isolates to ferment sugars.
This research was conducted in the Agriculture Faculty- Food Science Department, Damascus University, for detecting Lactic acid bacteria isolated from Syrian dairy products (white fresh cheese-Leben), Samples were collected from August, 2004 to Oc tober, 2005 from different areas in Syria. Analysis was done for 245 bacterial isolates from 40 samples of white fresh cheese and 58 samples of yogurt (curd). Results showed that the genus Enterococcus was dominant with a percentage of 47.52%; Pediococcus had a percentage of 26.3 %; while Lactococcus had a percentage of 15.38% with only 3.2% for Lactobacillus.
Sixty yeasts spp. were isolated from samples of whey which collected from different locations in Syria. These yeasts were classified morphologically according to Barnett’s and physiologically by API technique to identify the species of Yeasts, whe re had found 10 classified yeasts as follows: Candida krusei is the most of yeasts which founded in whey with 21.66 %, and in the Second rank is Candida lusitaniae with 16.66 %. the third rank came (Geotrichum capitatum, Saccharomyces cerevisiae, Rhodotorula minuta) with 11.66%, after that the Candida magnolia is ranked in the fourth rank by distributing with 8.33%. as soon as the Candida famata came in the fifth rank with 6.66%, and in the sixth rank we founded Candida lipolytica with 5%, finally the Rhodotorula. mucilaginosa and Candida. kefyr came in the last rank with 3.33%, so we noticed the variety of the yeasts in the studied locations in Syria.
أجري هذا البحث في مخابر كلية الزراعة بجامعة الفرات قسم علوم الأغذية وفي مخابر هيئة الطاقة الذرية بدمشق وقد هدف إلى عزل وتعريف بعض سلالات بكتريا حمض اللبن من الحليب الخام للنوق والماعز الشامي بالإضافة إلى دراسة بعض الخصائص الكيميائية والتصنيعية للسلالات المعزولة وذلك في عامي ( 2010و 2011)
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