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Register a new userMicropropagation of Five Date Palm Cultivars Through in vitro Axillary Buds Proliferation
التكاثر الخضري الدقيق بالَتبرعم لخمسة أصناف من نخيل الَتمر
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Damascus University ورقة بحثية
Publication date
1996
fields
Groves
and research's language is
العربية
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Shamra Editor
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جرى وضع تَِقانة للتكاثر الخضري الدقيق بتضاعف البراعم لخمسة أصناف من نخيل الَتمر هي: الخلاص، الهِلالي، الصْفري، الزهدي، و الرزيز. و تشمل هذه التَِقانة خمس مراحل هي: الزراعة الأولية، تكوين البراعم الجانبية، إكثار البراعم و استطالتها، تجذير النموات داخل الأنابيب و أخيرًا أَْقَلمة النباتات الناتجة (نقل النباتات إلى الأحوال الطبيعية). و في هذا البحث، جرى وصف سائر الشروط و الأوساط الغذائية المستعملة لمراحل الإكثار كافة. و قد تم الحصول على نباتات كاملة من سائر الأصناف المدروسة.
Tissue culture process of date palm propagation via bud proliferation is fully described. Adventitious plantlets were obtained from shoot tips. axillary buds and the bottom of young leaves of five date palm cultivars (Sefri, Zahdi, Rizzez, Khalas and Hillaly).
References used
Al-Maarri, K. and A.S. Al-Chamdi. ١٩٩٥. Effect of prelevement date on in vitro date Palm Phoenix dactylifera L.propagation on Hillaly. Arab. Univ. Agric. Sci
Ammar, S., and A. Benbadis. ١٩٧٧. Vegetative propagation of date palm Phoenix dactylifera L. by tissue culture of young plants derived from seeds. C.R. Acad. Sci. Paris
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This investigation was conducted on Ru140 grape rootstock at the General
Commission for Scientific Agricultural Research (GCSAR), Damascus with the
aim of in vitro vegetatively micropropagation using some plant growth
regulators on multiplication
and rooting to determine the best combinations
and concentrations of plant growth regulators that result in the best
multiplication rate, and best rooting crekeria (rate and roots number and
length). Results demonstrated that, the best medium for in vitro
micropropagation of the studied rootstock was the modified MS medium
supplemented with 4.44 μM BA + 0.49 μM IBA with multiplication rate of 7.72
new shoots every 4 weeks, and shoots lengt of 5.54 cm. These shoots were
transferred for 4 weeks to among elongation medium containing the same
medium with the addition of Kinetine at a concentration of 2.22 μM instead of
BA which led to a shoot elongation rate of 7.87 cm, then these shoots were
transferred to rooting medium for rooting, It was shown that using auxin IBA
at a concentration of 4.44 μM resulted at the highest rate of rooting (87%) with
the largest number of roots (7.56) when using the auxin IBA concentration μM
4.44 compared with the rest of other transactions and with the control as well.
However, The highest length of roots (6.29 cm) was observed on medim
contained lower IBA concentration (2.22 μM). Rooted Plants were acclimatized
gradually to ex vitro conditions with 70 % efficiency.
The objective of this research isto study the in vitro effect of some plant hormones
(growth regulators) BAP,NAA, 2,4-D, and genotype on callus and bud formation from
embryonic axes of two Soybean cultivar seeds (sb-44, sb-172).
The embryonic axes
were cultured on MS basal medium and MS supplemented with
1, 2, 3 mg/l BAP alone and in combination with 0.5 mg/l NAA.The cultures were
maintained at 25 C°±1 with photoperiod of 16 hours light (2000-2500 lux) and 8 hours
dark.The highest percentage92.5% and mean average 4.63 of callus formation were
recorded on MS medium containing BAP (3 mg/l) and NAA (0.5 mg/l).
The highest percentage 67.5% and average 3.38 of buds formation were obtained on
MS medium supplemented 1 mg/l BAP and 0.5 mg/l NAA in cultivar sb-44.
The percentage of callus formation increased, while the percentage of bud formation
decreased with each increase in BAP concentration when used alone. A positive increase
was observed in all mediums in the combination of 0.5 mg/l NAA or 0.5 mg/l 2. 4-D in the
two cultivars used in this study.
This study showed the genotypic difference effect on callus and bud formation.Roots
were formed from allplantlets cultured on MS medium without plant hormones.Rooted
plantlets were transferred into pots with nutrient soil, irrigated with water, and adapted
tolaboratory conditions. Good plants grown to maturity were obtained in 12-13 weeks.
This study was carried out at AL-Rasheedia Agricultural Research Station
,Ninevah, to evaluate five radish cultivars, during fall seasons (1997-1998) and
(1998-1999) .
Results showed significant differences among the different cultivars. The
cult
ivar Black (Rabeea) was superior to other cultivars in number of leaves/
plant, root diameter, weight, and total yield /donum, which were 11.81, 9.22
(cm), 382.91 (gram), and 11.67 ton/ donum respectivelly. The April Cross
cultivar gave the highest root length and foliar weight. Significant correlation
was observed between cultivars. Yield was significantly correlated with root
weight, number of leaves per plant and foliar weight. The root weight was
positively correlated with its diameter, and number of leaves per plant.
The present study was conducted on aerial offshoots of date palm
Berhi cultivar after separation from their mother trees, their bases were
dipped in solution of plant growth regulator (IBA).
A detailed in vitro multiplication system for rapid micropropagataion of the
Hypericum perforatum L. has been developed. Shoot tips and axillary buds
excised from this plant which were collected from Tartous explants and surface
disinifected then cultured on MS (Murashige and Skoog 1962) medium.
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