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Analysis of the Genetic Diversity in Syrian Shami Goats

تحليل التنوع الوراثي للماعز الشامي في سورية

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 Publication date 2013
and research's language is العربية
 Created by Shamra Editor




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References used
الخوري , فارس قيصر ( 1998) مداولات ندوة إنتاج الأغنام والماعز ,أكساد عمان
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Shami goat in Syria is one of the important domestic breeds, because of its high productivity and breeding capacity under the hard environmental conditions and it is still being used in crossbreeding with Mountain goats as a donor for milk produci ng traits. This breed is still far away from scientific investigations even it has been exposed to a random crossbreeding with Mountain goats by goat breeders. This has lead to the possibility of losing some of its genetic characteristics which are not detected yet. In this research the genetic diversity among groups of pure Shami goats was determined using ISSR-PCR on blood and results indicated that there was a molecular genetic diversity among studied goats and polymorphic rate was register to 100%.
The research aimed to evaluate the genetic diversity and the genetic relationship between 16 Hordeum vulgare genotypes (variaties and new lines), using SSR markers. 32 primer pairs were used in the analysis, five of them amplified primers monomorp hic alleles, while the remaining primers detected different alleles. A total of 126 polymorphic alleles were revealed by the 27 primer pairs. The number of different alleles detected on alocus ranged from 2 to 11with a mean of 4.66 alleles per locus. The polymorphic information content (PIC) values ranged from 0.21 to 0.88 with a mean value of 0.49, and the gene diversity ranged from 0.23 to 0.89 with a mean value of 0.53.The dendrogram was established using SSR data clustered into three major groups. The smallest genetic relationship was observed between line39 and line38 . The results proved the efficiency of SSR markers in the estimation of genetic diversity and in the clustering of the barley genotypes in Syria.
Random Amplified Polymorphic DNA (RAPD) was used to evaluate genetic diversity among 11 Yemeny pomegranate types. Samples were collected in 2004 from three main regions: east, middle and north of the country (Rada, Hada and Saada). PCR amplificati on of pomogranate DNA with 30 random primers generated 1013 bands, 118 of them were polymorphic 11.5%. Results showed that the genetic diversity within the tested types was limited. Similarity values ranged between 59 –96 % with a mean of 79 %. Statistical analysis showed that the types can be divided into two clusters: cluster 1 with Orkobi and Mellies types found in the Hada region; cluster 2 includes nine types belonging to the Saada and Radaa regions .High similarity (90–96%) was obtained with Khazemy, Liessy 1, Liessy 2, Taefi, Ahmer and Balady types. Whereas, some types could be considerd as separate cultivars due to their high genetic diversity such as, Orkoby and Mellies (Hada region) in addition to Bohssom, Sommaty and Liessy 3 in the Saada region.
This Study was carried-out at Shami Goat Improvement Station in Karahta. Ten growing Shami male goats, aged 8 months, housed and managed under the same conditions were used to study the semen characteristics. Artificial vagina was used to collect semen at weekly intervals, for 13 months to determine the volume, motility, pH, and sperm concentration using Neubaure- Spectrophotometer and Spermiodensimeter techniques. Overall averages and standard errors of volume, motility, concentration, and pH were 0.9 ± 0.07 ml, 65.24 ± 1.41%, 3.57 ± 0.14 billion/ml, and 6.52 ± 0.07, respectively. Seasonal variations were observed. A positive significant correlation between volume and motility (r= 0.42, P<0.01) was found, while negative correlations (P<0.01) between pH and other studied semen characteristics were recorded. Linear equations for detecting sperm concentration as measured by either, Spermiodensimeter or Spectrophotometer were obtained.
This Study was carried-out in Karahta station for improvement of Shami goats, Directorate of Animal Production Research, Ministry of Agriculture and Agrarian Reform. Fifty growing Shami male goats aged ٣ months, were used to measure the growth rat e of testicular parameters, their relation to the development of body weight and the effect of several factors on their development from weaning at ٣ months to ٢١ months of age. All kids housed and managed under the same conditions.

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