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Register a new userالإكثار الخضري لنبات التوليب المحلي ( Tulipa sp ) باستخدام تقنية زراعة الأنسجة
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Tishreen University رسالة ماجستير
Publication date
2015
fields
Agricultural Engineering
and research's language is
العربية
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هدف هذا البحث إلى إجراء التوصيف المظهري ودراسة درجة القرابة والتباين بين عدد من الطرز المحلية من التوليب (27) وتحديد عددها الصبغي
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الشاطر زهير والمحمود فادي 2010 دراسة بعض استخدامات التنوع الحيوي النباتي واهم النشاطات البشرية المؤثرة به في محمية أبي قبيس مجلة جامعة تشرين للبحوث والدراسات العلمية
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الإكثار الخضري الدقيق لنبات الويستيريا (Wisteria Sinensis) باستخدام تقنيات زراعة الأنسجة : أجري هذا البحث خلال الأعوام 2009-2012 في مخبر زراعة الأنسجة النباتية التابع لقسم البساتين في كلية الزراعة بجامعة حلب وفي البيت الزجاجي التابع للحديقة العامة في مدينة حلب
This present study was conducted to develop a detailed in vitro propagation
system for the medicinal shrub Capparis spinosa L.
Single nodes with one bud and a small part of stem of 1-1.5 cm long were
used as initial explants which were collected f
rom a shrubs grown under field
conditions at Damascus suburb., (Doumar). Explants were surface-disinfected
by 70% Ethanol for 1 min., followed by immersion in Sodium Hypochlorite or
HgCl2 for different periods and concentrations with 1 drop of Tween 20 for 100
ml disinfectant solution, where after, they were placed onto MS basal medium
containing a combination of growth regulators at different concentrations (BA
at 4.44 or 8.88 μM) each with IBA 0.49 μM. Cultures were incubated in the
growth room at 23±1 c and light intensity of 3000 lux at the cultures level.
Multiplication rate of 25.17-fold from one explant was achieved every 4 weeks
on the optimal MS medium (MS+8.88μM BA+0.49μM IBA).
The described method has potential to produce large numbers of plantlets
within a short period of time to expand its cultivation for medicinal uses.
The objective of this research is to propagate Mentha puligium in
vitro, shoot tips and nodal explant were planted with 0.5-1 cm length
on MS (Murashige's and Skoog) medium with various concentrations
(0.5-1-2-3-4) mg/L of cytokinin BAP.
A detailed in vitro multiplication system for rapid micropropagataion of the
Hypericum perforatum L. has been developed. Shoot tips and axillary buds
excised from this plant which were collected from Tartous explants and surface
disinifected then cultured on MS (Murashige and Skoog 1962) medium.
The present study was conducted at Genetic Engineering Department in Agricultural
Research Directorate in Ministry of Science and Technology/Iraq in 2013, to evaluate
the effectiveness of aqueous extract of cogon grass at concentrations of (0.0 ,0.
25,
0.5, 1, 1.5 and 2%) and Kinetin (0.0, 1.5, 3, 5) mg/L on microtuberization of two
potato varieties; Daimond and Desire, in two separate experiments. All cultures
were placed in a growth room chamber at temeratures of 18-20 °C with darkness
for 90 days, until microtubers harvest. Data of microtuber per plant, diameters and
weights were investigated. Results showed a positive effect of cogon grass extract
at 2% on the number of microtubers and fresh weight (2.1, 104.41 mg, respectively)
and at 1% on the diameter (5.52 mm). Desiree cultivar was the best in fresh weight
of microtubers (188.41mg). The results of kinetin showed a significant effect on
all of the studied traits. The superior kinetin concentration was 5.0 mg/L which
gave the highest number of microtubers 2.22, while the concentrations 3.0 and 1.5
mg/L gave the highest values of diameter and weight ( 6.95 mm and 388.38 mg,
respectively). The two cultivars did not differ significantly in number of microtubers,
while Diamond cultivar was significantly higher in diameter and weight (6.86 mm
and 363.45 mg, respectively).
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