The objective of this research isto study the in vitro effect of some plant hormones
(growth regulators) BAP,NAA, 2,4-D, and genotype on callus and bud formation from
embryonic axes of two Soybean cultivar seeds (sb-44, sb-172).
The embryonic axes
were cultured on MS basal medium and MS supplemented with
1, 2, 3 mg/l BAP alone and in combination with 0.5 mg/l NAA.The cultures were
maintained at 25 C°±1 with photoperiod of 16 hours light (2000-2500 lux) and 8 hours
dark.The highest percentage92.5% and mean average 4.63 of callus formation were
recorded on MS medium containing BAP (3 mg/l) and NAA (0.5 mg/l).
The highest percentage 67.5% and average 3.38 of buds formation were obtained on
MS medium supplemented 1 mg/l BAP and 0.5 mg/l NAA in cultivar sb-44.
The percentage of callus formation increased, while the percentage of bud formation
decreased with each increase in BAP concentration when used alone. A positive increase
was observed in all mediums in the combination of 0.5 mg/l NAA or 0.5 mg/l 2. 4-D in the
two cultivars used in this study.
This study showed the genotypic difference effect on callus and bud formation.Roots
were formed from allplantlets cultured on MS medium without plant hormones.Rooted
plantlets were transferred into pots with nutrient soil, irrigated with water, and adapted
tolaboratory conditions. Good plants grown to maturity were obtained in 12-13 weeks.
The objective of this research is to propagate Mentha puligium in
vitro, shoot tips and nodal explant were planted with 0.5-1 cm length
on MS (Murashige's and Skoog) medium with various concentrations
(0.5-1-2-3-4) mg/L of cytokinin BAP.
