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Register a new userIn vitro Propagation of Mentha pulegium
الإكثار الخضري مخبريا In vitro لنبات النعناع البري Mentha pulegium
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تم في هذا البحث دراسة الإكثار الخضري مخبريا باستخدام زراعة الأنسجة النباتية لنوع النعناع Mentha pulegium , حيث زرعت البراعم القمية و العقد الساقية بطول 0.5 , 1 سم على الوسط المغذي MS المضاف إليه 0.5 , 1, 2, 3, 4 مغ\ل من السيتوكينين BAP.
The objective of this research is to propagate Mentha puligium in vitro, shoot tips and nodal explant were planted with 0.5-1 cm length on MS (Murashige's and Skoog) medium with various concentrations (0.5-1-2-3-4) mg/L of cytokinin BAP.
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Research summary
يتناول هذا البحث دراسة الإكثار الخضري مخبرياً لنبات النعناع البري Mentha pulegium باستخدام تقنية زراعة الأنسجة النباتية. تم زراعة البراعم القمية والعقد الساقية بطول 0.5-1 سم على وسط MS المغذي مع إضافة تراكيز مختلفة من السيتوكينين BAP (0.5-1-2-3-4 مغ/ل). ومن ثم تم تجذير البراعم الناتجة على وسط MS مع إضافة تراكيز مختلفة من الأوكسين NAA (0.5-1 مغ/ل). أظهرت النتائج أن أفضل تركيز لتشكل البراعم كان 2 مغ/ل من BAP، حيث تم الحصول على 14 برعماً من البراعم القمية و10 براعم من العقد الساقية. أما أفضل تركيز لتشكل الجذور فكان 0.5 مغ/ل من NAA بنسبة 93%. نجحت عملية الأقلمة بنسبة 80% بعد نقل البادرات النامية إلى أصص تحتوي تورب مغذي. تهدف الدراسة إلى إنتاج نباتات على مدار العام دون التأثر بفصول السنة والحفاظ على كمية كبيرة من نباتات النعناع البري ذات الأهمية الطبية والاقتصادية.
Critical review
دراسة نقدية: يعتبر البحث مهماً في مجال الإكثار النباتي وتحديداً للنباتات الطبية ذات الأهمية الاقتصادية. ومع ذلك، يمكن تحسين البحث من خلال توسيع نطاق التجارب لتشمل أنواعاً أخرى من الهرمونات النباتية ودراسة تأثيرها على الإكثار. كما يمكن تحسين عملية الأقلمة من خلال تجربة أنواع مختلفة من التورب المغذي وتحليل تأثيرها على نسبة النجاح. بالإضافة إلى ذلك، يمكن تضمين دراسات مقارنة مع طرق أخرى للإكثار النباتي لتقديم رؤية شاملة حول فعالية التقنية المستخدمة.
Questions related to the research
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ما هو الهدف الرئيسي من البحث؟
الهدف الرئيسي هو إكثار نبات النعناع البري مخبرياً باستخدام تقنية زراعة الأنسجة النباتية ودراسة تأثير الهرمونات النباتية على تشكل البراعم والجذور.
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ما هي أفضل تركيزات السيتوكينين والأوكسين المستخدمة في الدراسة؟
أفضل تركيز للسيتوكينين BAP كان 2 مغ/ل، وأفضل تركيز للأوكسين NAA كان 0.5 مغ/ل.
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ما هي نسبة نجاح عملية الأقلمة للنباتات الناتجة؟
نسبة نجاح عملية الأقلمة كانت 80%.
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ما هي الفوائد الطبية والاقتصادية لنبات النعناع البري؟
نبات النعناع البري غني بالمعادن والفيتامينات والألياف والبروتين، ويحتوي على نسب عالية من الزيت الطيار pulegon الطبي، مما يجعله ذا أهمية طبية واقتصادية.
References used
ALAM, I. SHARMIN, S. A.; NAHER, M. K.; ALAM, M. J.; ANISUZZAMAN, M. & ALAM, M.F. 2010 . Effect of growth regulators on meristem culture and planlet establishment of sweet potato Ipomomea batatas (L.) Lam. Plant omics vol.3(2),p:35-39
ANISH, N.P.; RAJESH,M.G.; ELIAS,J. & JAYAN,N. 2010. In vitro propagation of solanum capsicoides all an important therapeutic agent kantakari. J. of plant tissue Culture & biology, vol. 20(2),p:179-184
BHAT, S.; MAHESHWAR, P.; KUMAR, S. & KUMAR, A. 2002. In vitro regeneration and trans formation. Molcular Biology, vol. 3(1): 11-23
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within a short period of time to expand its cultivation for medicinal uses.
This current study was carried out on B41 grape rootstock
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هدف هذا البحث إلى إجراء التوصيف المظهري ودراسة درجة القرابة والتباين بين عدد من الطرز المحلية من التوليب (27) وتحديد عددها الصبغي
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