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Register a new userMicropropagation of Hypericum perforatum
الإكثار الدقيق لنبات Hypericumperforatum
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Damascus University ورقة بحثية
Publication date
2013
fields
Biology
and research's language is
العربية
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Shamra Editor
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وضعت طريقة دقيقة لإكثار نباتHypericum perforatum في الزجاج من أجل الحصول على أعداد كبيرة من هذا النبات المهم طبياً. إذ أخذت عقل مفردة تحوي قمماً ناميةً و براعم جانبية من هذا النبات الذي جمِع من محافظة طرطوس، و أُجريت عملية التطهير السطحي للأجزاء النباتية و زرعت في أنابيب اختبار تحوي وسطاً مغذياً.
A detailed in vitro multiplication system for rapid micropropagataion of the Hypericum perforatum L. has been developed. Shoot tips and axillary buds excised from this plant which were collected from Tartous explants and surface disinifected then cultured on MS (Murashige and Skoog 1962) medium.
References used
Albert, Y; Foster steven. (1996). Encyclopedia of common natural ingredients, second edition, A. wily- interscience publication John wily and Sons, INC
Astarita, L and Santarém E. (2003). Multiple shoot formation in Hypericum perforatum L.and hypericin production Porto Alegre, RS, Brasil
Barnes Joanne; Linda, A; Anderson,J; Philipson David. (2001). St Jones wort (Hypericum perforatum): a review of its chemistry, pharmacology and clinical properties, journal of pharmacy and pharmacology 53, London P583-600
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Single nodes and axillary buds excised from adult trees of Myrtle (Myrtus
communis L.) grown in the field under natural conditions at Damascus
countryside (Ain Elfiegh) were used as primary explants, which were surfacedisinfected
by 70% Ethanol an
d Clorox containing 5.25 % Sodium Hypoclorite
with a drop of Tween 20 for different periods and concentrations before being
placed onto MS basal medium. Established cultures were then transferred onto
MS basal medium containing a combination of growth regulators at different
concentrations (BA at 2.22 and 4.44 μM) each with NAA at 0.54, 1.62, 5.4 μM
or 1.47 μM IBA with GA3 at 0.58 μM for all tratments. Multiplication rate of
12.8-fold was achieved every 4 weeks on MS medium supplemented with 4.44
μM BA with 1.47 μM IBA and GA3 at 0.58 μM.
Ziziphora canescens is a species of important medicinal plants in Syria due
to its medicinal properties as antibiotic, flavors and spices in various foods.
This plant is important, especially in folk medicine in some areas (Kalamoon)
on the one ha
nd, and retreat of its spread which may lead eventually to its
extinction on the other hand, so a protocol for rapid micropropagation has
been developing by using lateral and apical buds on nutrient media MS
supplemented with different types and concentrations of plant growth
regulators.
الإكثار الخضري الدقيق لنبات الويستيريا (Wisteria Sinensis) باستخدام تقنيات زراعة الأنسجة : أجري هذا البحث خلال الأعوام 2009-2012 في مخبر زراعة الأنسجة النباتية التابع لقسم البساتين في كلية الزراعة بجامعة حلب وفي البيت الزجاجي التابع للحديقة العامة في مدينة حلب
This study was conducted during 2012-2013 in order to efficiently micropropagate
viburnum (Viburnum tinus) bushes using tissue culture techniques. The shoots were
cultured on a Murashige and Skoog medium supplemented with 30 gl-1 sucrose, 7 gl-1 ag
ar
- agar, citric acid as an anti-oxidant (300 mgl-1), and different concentrations of benzyl
amino purine and Naphthalene Acetic Acid. A media of Murashige and Skoog was used
for laboratory rooting after reducing the major mineral elements to the half, reducing the
sucrose to 20 gl-1, and addingindole-3-butyric acid (IBA) of different concentrations (0,
0.5, 1, 1.5 mgl-1). The results showed that it is necessary to have auxin and cytokinin in
culture to improve the value of open buds and the number of shoots per initial explants.
The concentrations 0.25 mgl-1 from NAA with 1 mgl-1 from BAP gave the highest value of
open buds (93.33%) and the maximum number of shoot per initial explants (1.57). To
improve the number and length of the shoots produced, the solution mineral (MS) was
replaced with another: media woody plants (WPM) which gave better elongation for the
resulting growths (3.21 cm) and a better number of shoots (2.72 Growth/explant)
compared to the media (MS) using the same compatibility hormone (0.25 mgl-1 of NAA
and 1 mgl-1 of BAP).
The results also show that the highest percentage of rooting reached (84.44%) with
(0.5 mgl-1) IBA which was better than (1.5 mgl-1) IBA and better than the treatment of the
control. Results also showed that the best medium for the length and number of roots
formed was (2.7cm, 3.82root) when the concentration was (0.5 mgl-1) surpassing the
control. The success rate of the acclimatization of the resulting laboratory plantlets under
glasshouse conditions reached (73.33%) one month and a half after the transplanting.
This present study was conducted to develop a detailed in vitro propagation
system for the medicinal shrub Capparis spinosa L.
Single nodes with one bud and a small part of stem of 1-1.5 cm long were
used as initial explants which were collected f
rom a shrubs grown under field
conditions at Damascus suburb., (Doumar). Explants were surface-disinfected
by 70% Ethanol for 1 min., followed by immersion in Sodium Hypochlorite or
HgCl2 for different periods and concentrations with 1 drop of Tween 20 for 100
ml disinfectant solution, where after, they were placed onto MS basal medium
containing a combination of growth regulators at different concentrations (BA
at 4.44 or 8.88 μM) each with IBA 0.49 μM. Cultures were incubated in the
growth room at 23±1 c and light intensity of 3000 lux at the cultures level.
Multiplication rate of 25.17-fold from one explant was achieved every 4 weeks
on the optimal MS medium (MS+8.88μM BA+0.49μM IBA).
The described method has potential to produce large numbers of plantlets
within a short period of time to expand its cultivation for medicinal uses.
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