The aime of this study was to isolate and identify the seed borne fungi of two
varieties of Soybean, Sb172 and Sb44, using blotter method and P.D.A plate method.
21 species belong 10 genus were determined, saprophyte and pathogen Fungi.
P.D.A plat
e method showed the highest number of fungi in comparison with the
other method, and the fungus Aspergillus was the most frequent genus with a ratio of
44.2% for the variety sb172 and 45.14% for the variety sb44, followed by the fungus
Cladosporium and then Fusarium, whereas the genus Colletotrichum showed the lowest
frequent ratio (0.85%) with the variety Sb172.
The percentages of seeds germination and infection were differed between the two
varieties, and the ratio seeds germination of variety sb172 were higher than the variety
sb44 with the both methods of culture, and were equal 94% with prim variety and 92%
with the second ones by blotter method, whereas the ratio were 52% and 34% for both
varieties respectively by P.D.A plate method. However, the ratio of infection were, in
general, lower with prim variety than the second ones by the both method.
The results showed the seeds which were highest ratio infection appeared low ratio
germination .
In this research was studied the effect of different concentrations of (ethanolic –
methanolic – acetonic) extracts of Apium nodiflorum on growth of fusarium moniliforme
all extracts revealed clear inhibitory effect against this Fungi.
The inhibit
ory effect differenced as difference of parts of plant and the difference of
using concentrations from extract and a kind of solvent.
The MIC of different extracts ranged between 0.02 and 0.1 g/ml , The Acetonic
extract of stem was the more activity comparing with the other parts of plant inhibition was
completed at (0.02 g/ml) concentration.
For methanolic extract, the extract of whole plant and flowers was more activity and
the MIC was 0.08 g/ml for etanolic extract of flowers recorded the highest percentage of
inhibition incomparing with. The other parts of plant and it reached 0.06 g/ml.
The antagonistic activity of liquid culture filtrate of Trichodermaharzianum at
different concentrations (5, 10, 20 and 30%) was evaluated "in vitro" at 25 C° against
following plant and human pathogenic fungi: Aspergi
llusniger, Rhizopusstolonifer,
Fusariumoxysporum, F.moniliforme, Alternariaalternata and Candida albicans.
The results showed high level activity of culture filtrate inhibiting the growth of
tested fungi.
The activity was varied with the different species of fungi and the different
concentrations of culture filtrate. The highest activity was against fungus A.niger which
showed precent inhibition equal 96.3% at 30% concentration. Whereas the lowest
inhibition percentage to 77.6% was recorded for fungus A.alternata in comparison with
other fungi.
The culture filterate was not affected the radial colonies growth of R.stolonifer at 5%
and 10% concentrations, however the colonies was fragile and weak, and clearly inhibited
the spores formation.
The culture filtrate was showed high antagonistic capability against the human
pathogenic fungus C.albicans, and the radial inhibition growth was 1.38 cm at 30%
concentration.
The objective of this research is to propagate Mentha puligium in
vitro, shoot tips and nodal explant were planted with 0.5-1 cm length
on MS (Murashige's and Skoog) medium with various concentrations
(0.5-1-2-3-4) mg/L of cytokinin BAP.
