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In Vitro Propagation and In Vivo Acclimatization of Sour Orange (Citrus aurantium L.)

إكثار البرتقال الحامِض بوساطة زراعة الأنسجة و أَقَلمتِه خارج الأنابيب

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 Publication date 1998
and research's language is العربية
 Created by Shamra Editor




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Some factors which affect in vitro propagation of sour orange (Citrus aurantium. L.) were studied on Murashige and Skoog medium. Medium containing ١,٠ mg/L ٦-Benzylaminopurine (BA) was satisfactory for shoot multiplication, Dichlorophenoxyacetic acid (٢،٤-D) was not effective for shoot proliferation and it enhanced callus formation.


Artificial intelligence review:
Research summary
تتناول الورقة البحثية دراسة العوامل التي تؤثر على تكاثر البرتقال المر (Citrus aurantium L.) في المختبر باستخدام وسط Murashige و Skoog. تم استخدام -Benzylaminopurine (BA) بنجاح لتكاثر البراعم، بينما لم يكن Dichlorophenoxyacetic acid (-D) فعالاً في تكاثر البراعم بل زاد من تكوين الكالس. تم تحقيق نجاح في تكوين الجذور باستخدام Indole-3-acetic acid (IAA) أو -Naphthalenacetic acid (NAA). كان للفحم النشط (AC)، والفوروجليسينول (PG)، والبولي فينيل بيروليدون (PVP) تأثيرات متناقضة على تكوين الجذور عند إضافتها إلى وسط التجذير. تم تحقيق بقاء بنسبة عالية عند تكييف النباتات المجذرة في ظروف خارجية باستخدام خليط من البيتموس والبيرلايت تحت رذاذ ماء متقطع. نمت النباتات المتكيفة في الدفيئة وتم الحفاظ عليها خالية من الفيروسات.
Critical review
دراسة نقدية: تقدم هذه الورقة البحثية مساهمة قيمة في مجال تكاثر البرتقال المر في المختبر وتكييفه في الظروف الخارجية. ومع ذلك، يمكن تحسين الدراسة من خلال تقديم تفاصيل أكثر حول الظروف البيئية الدقيقة المستخدمة في التجارب، مثل درجة الحرارة والرطوبة. كما أن الدراسة لم تتناول بشكل كافٍ تأثير التغيرات في تركيزات المواد الكيميائية المختلفة على النتائج النهائية. بالإضافة إلى ذلك، كان من الممكن أن تكون الدراسة أكثر شمولاً إذا تم تضمين مقارنة مع طرق تكاثر أخرى للبرتقال المر.
Questions related to the research
  1. ما هي المواد الكيميائية المستخدمة لتكاثر البراعم في الدراسة؟

    تم استخدام -Benzylaminopurine (BA) بنجاح لتكاثر البراعم، بينما لم يكن Dichlorophenoxyacetic acid (-D) فعالاً.

  2. ما هي المواد المستخدمة لتكوين الجذور في الدراسة؟

    تم استخدام Indole-3-acetic acid (IAA) و -Naphthalenacetic acid (NAA) لتكوين الجذور.

  3. ما هي المواد التي أظهرت تأثيرات متناقضة عند إضافتها إلى وسط التجذير؟

    الفحم النشط (AC)، والفوروجليسينول (PG)، والبولي فينيل بيروليدون (PVP) أظهرت تأثيرات متناقضة عند إضافتها إلى وسط التجذير.

  4. كيف تم تكييف النباتات المجذرة في الظروف الخارجية؟

    تم تكييف النباتات المجذرة في ظروف خارجية باستخدام خليط من البيتموس والبيرلايت تحت رذاذ ماء متقطع، وتم نقلها لاحقاً إلى الدفيئة.


References used
Baralass, M., and K. G. M. Skene. ١٩٨٦. Citrus (Citrus species). In: Biotechnology in agriculture and forestry. By Bajaj. Y.P.S. (ed.) v. Vol. l. Trees. Sprigler-Verlag: Berlin and Heidelberg, pp
Baralass, M., and K. G. M. Skene. ١٩٨٢. In vitro plantlet formation from citrus species and hybrids. Sci. Hort
Bertrand-Desbrunais, A., M. Noriot and A. Charrier. ١٩٩١. Minimal growth in vitro conservation of coffee (coffea spp). Plant Cell, Tiss. Org. Cult
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This present study was conducted to develop a detailed in vitro propagation system for the medicinal shrub Capparis spinosa L. Single nodes with one bud and a small part of stem of 1-1.5 cm long were used as initial explants which were collected f rom a shrubs grown under field conditions at Damascus suburb., (Doumar). Explants were surface-disinfected by 70% Ethanol for 1 min., followed by immersion in Sodium Hypochlorite or HgCl2 for different periods and concentrations with 1 drop of Tween 20 for 100 ml disinfectant solution, where after, they were placed onto MS basal medium containing a combination of growth regulators at different concentrations (BA at 4.44 or 8.88 μM) each with IBA 0.49 μM. Cultures were incubated in the growth room at 23±1 c and light intensity of 3000 lux at the cultures level. Multiplication rate of 25.17-fold from one explant was achieved every 4 weeks on the optimal MS medium (MS+8.88μM BA+0.49μM IBA). The described method has potential to produce large numbers of plantlets within a short period of time to expand its cultivation for medicinal uses.
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