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Register a new userIn Vitro Propagation and In Vivo Acclimatization of Sour Orange (Citrus aurantium L.)
إكثار البرتقال الحامِض بوساطة زراعة الأنسجة و أَقَلمتِه خارج الأنابيب
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جرت دراسة بعض العوامل المؤثرة في إكثار البرتقال الحامِض على الوسط الغذائي لموراشيج و سكوج. أظهرت النتائج أن استعمال (١مغ/لتر) من البينزيل أدينين (BA) كان مناسبًا للإشطاء و التفرع، و لكن مادة ٤،٢ -د لم تكن ذات تأثير في الإشطاء في حين كانت مشجعة لتكون الكالوس.
Some factors which affect in vitro propagation of sour orange (Citrus aurantium. L.) were studied on Murashige and Skoog medium. Medium containing ١,٠ mg/L ٦-Benzylaminopurine (BA) was satisfactory for shoot multiplication, Dichlorophenoxyacetic acid (٢،٤-D) was not effective for shoot proliferation and it enhanced callus formation.
References used
Baralass, M., and K. G. M. Skene. ١٩٨٦. Citrus (Citrus species). In: Biotechnology in agriculture and forestry. By Bajaj. Y.P.S. (ed.) v. Vol. l. Trees. Sprigler-Verlag: Berlin and Heidelberg, pp
Baralass, M., and K. G. M. Skene. ١٩٨٢. In vitro plantlet formation from citrus species and hybrids. Sci. Hort
Bertrand-Desbrunais, A., M. Noriot and A. Charrier. ١٩٩١. Minimal growth in vitro conservation of coffee (coffea spp). Plant Cell, Tiss. Org. Cult
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In vitro rooting was significantly increased by adding indolebutyric
acid (IBA) to medium and rooting was improved by adding ١,٠ g/L activated
charcoal or ٠,١ g/L polyvinylpyrrolidone (PVP). Indoleacetic acid (IAA) (٠,٠ to
٤,٠ mg/L) was not effect
ive for rooting except when used with ٠,١ g/L PVP. A
٩٥٪ survival was achieved when plants were acclimatized ex vitro. Such
procedures could help significantly in clonally propagating bitter almond and
conserving its germplasm.
A successful and detailed in vitro propagation system for rapid
micropropagation of three apple rootstocks: MM ١٠٦, EM ٧ and M ٢٦ has
been developed. Shoot tips and axillary buds excised from field-grown trees
were used as explants, which were surface-disinfected with mercury bichloride,
or with solution of sodium or calcium hypochlorite followed by three rinses
with sterile distilled water.
Effect of Citrus trestiza virus (CTV) in Growth of Balady common orange and
Satsuma trees grafted on sour orange Rootstock in Hraisoon during 2013 was studied. The
results showed that of infection by CTV caused deformation of shaped leaves, boat or
spoon-shaped leaves. The symptoms was greater in Satsuma from Balady common orange
trees. Also leaf size average of Balady common orange trees reduced from 15.58 cm2 in
healthy trees to 11.82 cm2 in infection trees as percentage 24.13, and from 19.64 cm2 in
healthy Satsuma trees to 12.38 cm2 in infection trees as percentage 36.97. and length
average of spring, summery and autumnal fresh foliages growths was reduced from 20.98
cm, 14.62 cm, 12.17cm in healthy Balady common orange trees to18.75cm, 12.52cm, 9.32
cm in infection trees respectively. Also it reduced from 18.78 cm, 14.56 cm, 10.06 cm in
healthy Satsuma trees to 13.78 cm, 9.34 cm, 6.03 cm in infection trees respectively, the
CTV had no significant effect on Trunk circumference in both varieties.
This present study was conducted to develop a detailed in vitro propagation
system for the medicinal shrub Capparis spinosa L.
Single nodes with one bud and a small part of stem of 1-1.5 cm long were
used as initial explants which were collected f
rom a shrubs grown under field
conditions at Damascus suburb., (Doumar). Explants were surface-disinfected
by 70% Ethanol for 1 min., followed by immersion in Sodium Hypochlorite or
HgCl2 for different periods and concentrations with 1 drop of Tween 20 for 100
ml disinfectant solution, where after, they were placed onto MS basal medium
containing a combination of growth regulators at different concentrations (BA
at 4.44 or 8.88 μM) each with IBA 0.49 μM. Cultures were incubated in the
growth room at 23±1 c and light intensity of 3000 lux at the cultures level.
Multiplication rate of 25.17-fold from one explant was achieved every 4 weeks
on the optimal MS medium (MS+8.88μM BA+0.49μM IBA).
The described method has potential to produce large numbers of plantlets
within a short period of time to expand its cultivation for medicinal uses.
In this study, we used plant tissue culture techniques for micropropagation
of the endangered Red Doumani cultivar. The explants which were the apex
tips and axillary buds (0.5-1 cm) have been cultured on the free of hormones
initial medium WPM (W
oody plant medium), then moved to the
micropropagation media. The results showed that the medium containing 4.44
mM BA + 0.58 mM IAA had the best effect on number of new shoots formed
(2.6) and the growth started after 9.22 days, the best elongation was on the
medium supplemented with 4.6 mM KIN + 0.58 mM IAA and the rate of
multiplication reached 5.15 shoots.
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