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Register a new userThe In Vitro Propagation of Three Apple Rootstocks
إكثار بعض أصول التفاح الخضرية بوساطة زراعة النسج
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Damascus University ورقة بحثية
Publication date
2000
fields
Biology
and research's language is
العربية
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Shamra Editor
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نفّذ هذا البحث في مخبر زراعة الأنسجة النباتية بمديرية البحوث العلمية الزراعية، حيث استخدمت تقانة زراعة الأنسجة النباتية لإكثار بعض أنواع أصول التفاح خضريًا ( الأصل ١٠٦ MM نصف مقصر, الأصل 7M نصف مقصر, الأصل 26 M, مقصر قزم).
A successful and detailed in vitro propagation system for rapid micropropagation of three apple rootstocks: MM ١٠٦, EM ٧ and M ٢٦ has been developed. Shoot tips and axillary buds excised from field-grown trees were used as explants, which were surface-disinfected with mercury bichloride, or with solution of sodium or calcium hypochlorite followed by three rinses with sterile distilled water.
References used
Adams, R.L., S.S. Koenigsberg, and R.W. Langhans. ١٩٧٩. In vitro propagation of Cephalotus pllicularis (Australian pitcher plant). Hort. Sci ١٤: ٧٠١-٧٠٢
Cheema, G.S., and D.P. Sharma. ١٩٨٣. In vitro propagation of apple rootstock ELMA ٢٥. Acta Hort. ١٣١: ٧٥-٨٨
Donelly, D.J., and W.E. Vidaver. ١٩٨٨. Glossary of plant tissue culture Belhaven Press. London
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Some factors which affect in vitro propagation of sour orange (Citrus
aurantium. L.) were studied on Murashige and Skoog medium. Medium
containing ١,٠ mg/L ٦-Benzylaminopurine (BA) was satisfactory for shoot
multiplication, Dichlorophenoxyacetic acid (٢،٤-D) was not effective for shoot
proliferation and it enhanced callus formation.
In vitro rooting was significantly increased by adding indolebutyric
acid (IBA) to medium and rooting was improved by adding ١,٠ g/L activated
charcoal or ٠,١ g/L polyvinylpyrrolidone (PVP). Indoleacetic acid (IAA) (٠,٠ to
٤,٠ mg/L) was not effect
ive for rooting except when used with ٠,١ g/L PVP. A
٩٥٪ survival was achieved when plants were acclimatized ex vitro. Such
procedures could help significantly in clonally propagating bitter almond and
conserving its germplasm.
The study was performed in Barshin Research Station- General
Commission for Scientific Agriculture Research on the trees age of
13 in grafted with Golden Delicious Cultivar on three rootstocks
(seedless rootstock Malus domestica, vegetative strong
rootstock
MM109, and MM111 half strong rootstock) over the years
(2014.2015 ).The study focused on growth vigor (the stem
circumference above the grafted area ,crown size - the rate of
shoots length and productivity).
This study was conducted for two successive seasons 2010 and 2011 in the
vine nursery in the governorate of Homs/Syria to evaluate the degree of
grafting success between the two partners to create graft combinations in early
stage of grafting and
to select the most compatible rootstock for our local
studied cultivars. Three grapevines of local cultivars (Halawani, Baladi and
Bayadi) were grafted on four different American rootstocks (Ru140, B41,
Fercal and SO4) using bench grafting by omega machine.
This study was carried out on three rootstocks of Crataegus monogyna Jacq.
With one seed containing fruit C.oxycantha L. with three seeds as well as C.
azaraolus Borkh, to identify the best method of Crataegus propagation.
The hardwood cuttings of
C.azarolus Borkh rooted in a IBA solution (4ooo
ppm)showed the highest rooting percentage 20% when grown in sand as well as
peat moss containing fermented arganic manure media.
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