تم تجذير النموات الخضرية بإضافة حامض أندول بيوتريك كما تحسن التجذير باستخدام ١,٠ مغ/لتر من مادة بولي فينيل بيرو ليدون، و لم يؤثر استعمال حامض اندول استيك (صفر – ٤,٠ مغ/لتر) في التجذير إلا عندما استعمل معه ٠,١ مغ/لتر من مادة بولي بيرو ليدون، و قد حصلنا على درجة نجاح ٩٥ % عند أقلمة النبات خارج الأنابيب. و يمكن أن تسهم نتائج هذه الدراسة في الإكثار الخضري للوز المر و المحافظة على هذه المادة الوراثية المهمة داخل الأنابيب.
In vitro rooting was significantly increased by adding indolebutyric
acid (IBA) to medium and rooting was improved by adding ١,٠ g/L activated
charcoal or ٠,١ g/L polyvinylpyrrolidone (PVP). Indoleacetic acid (IAA) (٠,٠ to
٤,٠ mg/L) was not effective for rooting except when used with ٠,١ g/L PVP. A
٩٥٪ survival was achieved when plants were acclimatized ex vitro. Such
procedures could help significantly in clonally propagating bitter almond and
conserving its germplasm.
References used
Bajaj, Y.P. ١٩٨٦. Biotechnology in agriculture and forestry. Trees I. Spring- Verlag, Berlin, Heidelberg
George, E.F., and P.D. Sherrington. ١٩٨٤. Plant propagation by tissue culture. Exegetics Ltd. Edington, Westbury, Wilts. England
Some factors which affect in vitro propagation of sour orange (Citrus
aurantium. L.) were studied on Murashige and Skoog medium. Medium
containing ١,٠ mg/L ٦-Benzylaminopurine (BA) was satisfactory for shoot
multiplication, Dichlorophenoxyacetic acid (٢،٤-D) was not effective for shoot
proliferation and it enhanced callus formation.
A successful and detailed in vitro propagation system for rapid
micropropagation of three apple rootstocks: MM ١٠٦, EM ٧ and M ٢٦ has
been developed. Shoot tips and axillary buds excised from field-grown trees
were used as explants, which were surface-disinfected with mercury bichloride,
or with solution of sodium or calcium hypochlorite followed by three rinses
with sterile distilled water.
Single nodes and axillary buds excised from adult trees of Myrtle (Myrtus
communis L.) grown in the field under natural conditions at Damascus
countryside (Ain Elfiegh) were used as primary explants, which were surfacedisinfected
by 70% Ethanol an
This present study was conducted to develop a detailed in vitro propagation
system for the medicinal shrub Capparis spinosa L.
Single nodes with one bud and a small part of stem of 1-1.5 cm long were
used as initial explants which were collected f
This research was carried out to determine the quantity of essential oil extracted
from myrtle Myrtus communis L leaves, by hydro distillation, and to identify the
major and minor components, besides to determine its quantities in the essential oil