In this study, we used plant tissue culture techniques for micropropagation
of the endangered Red Doumani cultivar. The explants which were the apex
tips and axillary buds (0.5-1 cm) have been cultured on the free of hormones
initial medium WPM (W
oody plant medium), then moved to the
micropropagation media. The results showed that the medium containing 4.44
mM BA + 0.58 mM IAA had the best effect on number of new shoots formed
(2.6) and the growth started after 9.22 days, the best elongation was on the
medium supplemented with 4.6 mM KIN + 0.58 mM IAA and the rate of
multiplication reached 5.15 shoots.
