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Register a new userUsing PCR technique to detect the Giardia on vegetables and irrigation water in Damascus Countryside
استخدام تقانةالـ PCR في الكشف عن الجيارديا على الخضار و المياه المروية في ريف دمشق
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ُنفذ البحث في الموسم 2011-2012 في مخابر قسم التقانات الحيوية التابع للهيئة العامة للبحـوث العلمية الزراعية بهدف الكشف عن وجود بيوض Giardia على 120 عينة شملت 40 عينة ميـاه ري و 80 عينة خضار جمعت عشوائياً من عشر مناطق زراعية مختلفة ضمن محافظة ريف دمشق باستخدام تقانة الـPCR .بينت نتائج الدراسة بعـد فـصل نـواتج تفاعـل PCR أن النـسبة المئويـة للتلـوث بـِ Giardia كانت %8.25 ، موزعةً على %5.37 في عينات مياه الري و %20 في عينات الخـضار. تَبين وجود بيوض النوع lamblia. G في 15 عينة من أصل المجموع الكلي للعينـات توزعـت علـى 7 عينات مياه ري و 8 عينات خضار. أعطت منطقة مسرابا أعلى نسبة تلـوث %6.41 بالنـسبة إلـى الجنس Giardia و %25 بالنسبة إلى النوع lamblia. G و دون فرق معنوي مع منطقة الكفرين التـي أظهرت أقـل نـسبة تلـوث (33.8%) بالنـسبة لِــ Giardia ،G و خلوهـا مـن بيـوض النـوع lamblia. G .أكدت هذه الدراسة أهمية تطبيق طرائق البيولوجيا الجزيئية في الكشف عن وجـود هـذا الطفيلي على الخضار و المياه المروية بها، و قد أظهرت أزواج المرئسات المستخدمة نوعيـة و حـساسية عاليتين في الكشف عن الجنس Giardia و تمييز النوع الممرض للإنسان lamblia. G.
This investigation was carried out during 2011–2012 season at the biotechnology department\General Commission for Scientific Agricultural Researches in order to detect the presence of Giardia Cysts on fresh vegetables and irrigation water using PCR technique to detect the status of Giardia cysts in ten different agricultural areas of Damascus Countryside. 120 samples including, 40 irrigation water and 80 vegetables samples were randomly collected and tested. Out of 120 samples examined, only 25.8 % were contaminated with Giardia cysts and these included 37.5% of irrigation water samples and 20% of fresh vegetables samples. G. lamblia cysts were detected in 12.5% of examined samples, 17.5% of irrigation water samples and 10% of fresh vegetables samples. The majority of contaminated samples (41.6%), (25%) for Giardia and G. lamblia, respectively were from Misraba area without statistically significant with Kafrein area, which showed the lowest contamination with Giardia cysts (8.33%) and was free of G. lamblia cysts. This study confirmed the importance of application of molecular biology methods in the detection of Giardia on vegetables and their Irrigation water, since used primers showed high specificity and sensitivity in the detection of the Giardia genus and discriminate the Human pathogenic type, G. lamblia.
References used
Abougrain, A. K., Nahaisi, M. H., Madi, N. S., Saied, M. M. and Ghenghesh, S. G. (2009). Parasitological contamination in salad vegetables in Tripoli- Libya. Food Control , 26:500-503
Adam, R. D. (1991). The biology of Giardia spp. Microbiological Reviews 55, 706–773
Al-Binali, A. M., Bello, C. S., EleShewy, K., and Abdulla, S. E. (2006). The prevalence of parasites in commonly used leafy vegetables in South Western, Saudi Arabia. Saudi Medical Journal, 27: 613-616
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