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Changes in the amount of proteins in seeds and stems cells of seedlings of Maize Plant (Zea mays L.) cultured in solutions of some heavy metal salts

تغيرات كمية البروتينات في بذور سويقة بادرات الذرة الصفراء .Zea mays L و خلاياها النامية في محاليل بعض أملاح المعادن الثقيلة

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 Publication date 2014
and research's language is العربية
 Created by Shamra Editor




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The aim of this research is to determine the effect of some heavy metal ions on total protien metabolism in seeds and seedlings of maize plant (GHOTA 82) and study the changes in the morphological specifications of plant, after cultivating for 6 days in solutions at different concentrations of water and heavy metal ions Ni2+, Cu2+, Zn2+, Cd2+, Hg2+ and Pb2+. The results showed that heavy metal ions have inhibitory effect on total protien metabolism, which decreased their amount in seedlings, While accumulated in the seeds. This inhibitory effect depends on the type and concentration of heavy metal ions. The results also showed morphological changes in the maize plant seedlings, which decreased their growth due to the effect of these ions.



References used
Mohan, B and Hosetti, B; 2006. Phytotoxicity of cadmium on the physiological dynamics of Salvinia natans L. grown in macrophyte ponds, Journal of Environmental Biology, 27(4) 701-704
Zengin, F and Kirbag, S; 2007. Effects of copper on chlorophyll, proline, protein and abscisic acid level of sunflower (Helianthus annuus L.) seedlings, Journal of Environmental Biology, 28(3) 561-566
Bandita, D and Preetam, N; 2011. Study of copper phytotoxicity on in vitro culture of Musa acuminata cv. ‘Bantala, Journal of Agricultural Biotechnology and Sustainable Development, Vol. 3(8), pp. 136-140
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This study was carried out in the farm of the Faculty of Agriculture University of Damascus during the growing season 2014, to study the productivity of the maize cultivar ("Gouta 82) and the hybrids (Basel 1 and Basel 2) under the influence of tw o plant densities 17.53 thousand plant. ha-1 (70x20cm) and 53.11 thousand plant. ha-1 (70x40cm).
This research aims to study the proteins transformation in zea maize plant seedlings cells, (GHOTA 82) under the influence of phyto hormone Auxin & grothregulators 2,4-D and IBA. Maize plant seeds were germinated in water medium for six days in d arkness and temperature of 25 ºC. The quantity different groups of proteins, were analyzed in the stem cells of the developing seedling mesochotyles after incupation in darkness water and in different solutions of auxin, 2, 4D & IBA (in concentration 50 mg/l) for 20 hours in 26 ºC. The results showed that had inhibition effects on hydrolysis of protein groups in zea maize plant seedlings cells.
A pot exrpriment was conducted in a greenhouse in Tishreen University. The experiment included three soils differ in their chemical properties: 1) heavy clay red soil rich in iron oxides; 2) a silty loam basiltic soil; 3) a heavet clay red soil ric h in total calcium carbonate (34.8 %). Treatments include application of different levels of triple super phosphate (TSP, 46 % P2O5) ranged from 0, 20, 40, 60, 80, 100, 150, to 200 mg P/kg soil. Pots were sowned with maize (2 plants/pot) and grown for 48 days. At harvest, plant were hatvested before flowering by cutting shoots and extracting roots which were oven dried and weight were recorded prior to digestion and P determination in shoot and root tissues. Samples of rhizosphere soils from each pot replicate were taken for P fractionation and determination. The response of maize to P application differs at period test according to soil type. The increase in growth was linear with increasing P level of application in theheavy red and calcareous red soils, while was polynomial in basilitic soil. The speed of growth increase in response to P application was influenced by native available P in the soil prior to application. The application of P led to linear increase in resin-P and MRP fraction in the bicarbonate extract, but not the Po fraction of the bicarbonate extract. The quantities of fixed applied P varied according to level of P application, the ratio of P uptake by maize plants, and chemical properties of the soil type. The proportion of applied P that was fixed increased linerarly with increasing level of P application. It constitutes 41.7,68.5 and66% at level of P application (20 mg/kg soil), and decreasing to30.2, 41.9 and 59.1% at level of P application (200 mg/kg soil) for red, basiltic, and calcerous red soils, respectively.
The research was conducted at the Maize Researches Department, General Commission for Scientific Agriculture Researches (G.C.S.A.R.) Damascus, Syria during the summer growing seasons of 2010, 2011 and 2012. Treatments were arranged in a Randomized Complete Blocks Design with three replications. The research aimed to evaluate genetic parameters for some traits like days to 50% silking, plant and ear height, ear length, ear diameter, number of rows per ear, number of kernels per row, 100 kernel weight and grain yield per plant using generations means analysis of two maize hybrids (IL.292-06 × IL.565-06, IL.459-06 × IL.362-06) to detect epistasis and estimates of mean effect [m], additive [d], dominance [h], additive × additive [i], additive × dominance [j] and dominance × dominance [l] parameters. Results showed that the additive - dominance model was adequate to demonstrate the genetic variation and its importance in the inheritance of most studied traits. Nonallelic gene interaction was operating in the control of genetic variation in most studied traits. The signs of [h] and [l] were opposite in most studied traits for the two crosses. Also, the inheritance of all studied traits was controlled by additive and non-additive genetic effects, but dominance gene effects play the major role in controlling the genetic variation of the most studied traits, suggesting that the improvement of those characters need intensive selection through later generations. The phenotypic variations were greater than genotypic variations for all studied traits in the two crosses, indicating greater influence of environment in the expression of these traits. Highly significant heterosis relative to mid and better parents, respectively was found for all characters, and this accompanied with inbreeding depression for all traits. Narrow sense heritability and genetic advance were low in most of the traits due to the dominance of non-additive gene action in controlling the genetic variation of the most studied traits and this predict low to medium values of genetic advance through selection process.
This study was conducted in Abu-Jarash farm at the Faculty of Agriculture, Damascus University during the main season 2013 to evaluate the effect of soil solarization and organic fertilization treatments on the productivity of some maize genotypes (Basel 1, Basel 2, Ghouta 1, Ghouta 82 and Local White). The experiment was laid out according to split-split plot design with three replications. The statistical analysis results clearly indicated to the existence of variability in the response of studied maize genotypes to soil solarization and organic fertilization treatments. The genotype Ghouta 82 surpassed in the traits of plant height, leaf area index, 100-kernel weight and grain yield (150.25 cm, 2.84, 28.10 g and 7.99 ton.ha-1) respectively, as compared to other studied genotypes and was closely followed by the genotype Basel 2 which recorded the highest number of rows per ear (13.83 row/ear). Soil solarization for 45 days before sowing and applying organic manures at the rate of 20 ton.ha-1 encouraged the traits of plant height, leaf area index, number of rows and grains per ear, 100-kernel weight which was reflected on grain yield. Therefore we can grow variety Ghouta 82 or hybrid Basel 2 to get higher yield of maize.

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