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Register a new userIsolation and identification of Pseudomonas pachastrellae, Micrococcus brunensis bacteria from soil polluted by petroleum
عزل و تصنيف النوعين الجرثوميين Pseudomonas pachastrellae Micrococcus brunensis من تربة ملوثة بمركبات نفطية
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أميمة ناصر( باحث )
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عُزل نوعان من الجراثيم من ترب ملوثة بمركبات نفطية, تم تمييز العزلتين الجرثوميتين ببعض الخصائص اعتماداً على تلوينها بطريقة غرام, و بعض الخصائص الشكلية و الكيميائية الحيوية. بينت النتائج عزل نوعان من الجراثيم, النوع الأول عصوي قصير سالب صبغة غرام, تميز بتفاعل سلبي تجاه اختباري اليوريا و النترات, و تفاعل إيجابي بالنسبة لاختبار سكر الغلوكوز فقط, و النوع الثاني من جراثيم المكورات العنقودية موجب صبغة غرام, تميز بتفاعل سلبي تجاه اختباري الجلاتين و استعمال السترات, كما أعطى تفاعل سلبي بالنسبة لاختبار سكر المالتوز و المانيتول, و استناداً إلى تصنيف بيرجي, و خلال فترة حضانة استمرت (48-72) ساعة, و عند درجة حرارة (37) درجة مئوية. و الجراثيم المعزولة هي نوعان: الأول Pseudomonas pachastrellae , و الثاني Micrococcus brunensis.
Two bacterial species were isolated from petroleum hydrocarbon-contaminated soil. The isolates were characterized based on their gram reaction characteristics, morphological and biochemical properties. The results showed two bacteria isolated the first isolated: gram negative short rod, urease and nitrate reduction test showed a positive result , and glucose test showed positive result. And the second isolated: gram positive cocci, gelatin and Citrate utilization test showed a positive result , and maltose, mannitol test showed negative result. They were then incubated at (37) ° C for (48- 72) hours. This is based on comparison of results with Bergey Manual of systematic bacteriology. The isolates were identified to be: Pseudomonas pachastrellae (X1) Micrococcus brunensis (X2).
References used
DIYAUDDEEN, B. W; WANDAUD, A. A. Treatment Technologies for Petroleum Refinery Effluents: A Review. Process Safety and Environmental Protection, 2011, 89: 95–10
GARGOURI, B. F; KARRY, N; MHIRI, F; ALOUI, S. Application of a Continuously Stirred Tank Bioreactor (CSTR) for Bioremediation of Hydrocarbon-Rich Industrial Wastewater Effluents. Journal of Hazardous Materials, 2011,189: 427–434
ADEKUNLE, A. A; ADEBAMBO, A. O. Petroleum Hydrocarbon Utilization by Fungi Isolated from Detarium Senegalense (J. F Gmelin) Seeds. Journal of American Science, 3(1), 2007
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