Isolation and identification of Pseudomonas aeruginosa was screened from
15 samples of Syrian oily-soils to determine the ability to produce Lipase
enzyme by Cetermide Agar, Tributrin Agar and Brain Heart Broth during
2007-2008 AD. The results sho
wed that there were grown colonies with color of
green or yellow-green surrounded with different diameter of clear zone in three
samples and the identification by Mowasat Hospital revealed that it was
Pseudomonas aeruginosa In addition, the results showed that the optimum
conditions to produce lipase was 35 Cº with the pH degree of 8 and the time of
production of lipase with highest activity of 63.3% was three days in
submerged culture during the optimum conditions. These results illustrated the
important of this isolates to produce and control lipase for industrial
production.
