Effective Factors of Dormant Seeds Lipase Activity in Gota Castor Bean

The present work was undertaken to study the properties and varations in the activities of castor bean lipase under different condition. Enzymes were extracted from dormant seed by phosphate buffer, then characterizations were conducted.

Isolation and Identification of Pseudomonas aeruginosa From Syrian Oily-soils and Evaluation its Production for Lipase

Isolation and identification of Pseudomonas aeruginosa was screened from 15 samples of Syrian oily-soils to determine the ability to produce Lipase enzyme by Cetermide Agar, Tributrin Agar and Brain Heart Broth during 2007-2008 AD. The results showed that there were grown colonies with color of green or yellow-green surrounded with different diameter of clear zone in three samples and the identification by Mowasat Hospital revealed that it was Pseudomonas aeruginosa In addition, the results showed that the optimum conditions to produce lipase was 35 Cº with the pH degree of 8 and the time of production of lipase with highest activity of 63.3% was three days in submerged culture during the optimum conditions. These results illustrated the important of this isolates to produce and control lipase for industrial production.

The ability of some local fungal isolates in enzyme lipase production and determine optimal growth conditions for its production By Penisillium devirsum

It wasdetected for the ability of a local fungus A.niger, A.alternata, F.oxysporum, F.solaniandP.devirsum to produce the enzyme lipase in solidmedium including Triputyrin. The enzymic activity of P.devirsum fungus is distinction from other fungus, while the fungus A.alternata failed to produce the lipase. Then they tested quantitatively using a liquid center to determine the best conditions for best growth to fungus P.devirsum and for best produce to enzyme lipase. It indicates that corn oil is the best carbonic source for growth of fungus after 5 days of incubation, as biomass reached(15.99g/L) and effective of lipase (67.43%).And it found that the concentration of 2% of the corn oil has achieved best biomass (17.83g/L) and best production of enzyme lipase effectively(72.78%). Temperature 34 c achieved the highest biomass (18.94g/L) and best production of enzyme lipase (76.16%), and pH =7 was the best for the growth of fungus in biomass reached (21.87g/L) and production of lipase effectively (82.93%). While the nitrogenic source gave peptone biggest value to biomass (27.08 g/L) and highest production for enzyme (88.12%) in optimal conditions reached.