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Register a new userIsolation of Antagonistic Bacteria against the Casual of White Rot Sclerotinia sclerotiorum from some Plant Roots, Sclerotia and Soil
عزل بكتريا من جذور بعض النباتات و الأجسام الحجرية و التربة ذات تأثير مضاد في الفطر sclerotiorum Sclerotinia
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جمعت عينات من جذور نبات الخس و الجزر و الأجسام الحجريـة للفطـر الممـرض Sclerotinia sclerotiorum و التربة من المنطقة الجنوبية من دمشق (الطيبة، الدرخبية، زاكية، المقيلبة) خلال العـام 2001 ، و عزلت 82 عزلة بكتيرية، منها 48 عزلة من الجذور، و 27 من سطح الأجسام الحجرية، و 7 من التربة المحيطة بالجذور. ثم اختبرت صفة التضاد لهذه العزلات البكتيرية اتجاه المسبب الممرض للعفـن الأبيض في المختبر vitro In . حيث وجد أن 7 عزلات بكتيرية كان لها تأثير مـضاد فـي نمـو الفطـر الممرض و تراوحت مسافة التضاد ما بين 3.1-11 ملم، و قد حققت العزلة 4.K82 المعزولة مـن التربـة المحيطة بالجذور أكبر مسافة تضاد بين نموها و نمو الفطر الممرض 11 ملم. و أمكن تعريف ثلاث عزلات بكتيرية منها و هي: 4.K82 المعزولة من تربة الخس، و FN3 المعزولة من سطح الأجسام الحجرية مـن حقول الخس، و 4.K4 المعزولة من جذور نبات الجزر، و هي تنتمي إلى جنس .spp Bacillus .
Samples from carrot and lettuce roots, sclerotia of Sclerotinia sclerotiorum, and soil were collected from south of Damascus during 2001. 82 isolates were isolated, 48 of them were isolated from roots, 27 from sarfuce of sclerotia, and 7 from soil around roots. Antgonisim capacity of these isolates was tested against S. Sclerotiorum in vitro. 7 bacterial isolates showed antagonistic effect on the growth of S. Sclerotiorum, the antagonism distance ranged bettwen (1.3 – 11 mm). The Isolate (K82.4), which was isolated from soil, showed antagonistic distance (11mm), and was the best of bacterial isolates. Three isolates were iddentified (K82.4 isolated from lettuce soil, FN3 isolated from surface of sclerotia, K4.4 isolated from carrot roots), that belong to Bacillus spp.
References used
Agrios, G. N. 1997. Sclerotinia Diseases of Vegetables and Flowers. Pages 355-358. In Plant Pathology. 4th. Edition. Academic Press. San Diego, London, Boston, New York, Sydney, Tokyo, Tornto
Cassiolato, A. M. R. 1998. Effects of Coniothyrium minitans on carpogenic germination and viability of Sclerotinia sclerotiorum sclerotia. Fitopatologia Brasileira 23: 474-476
Dillard, H. R, Ludwing, G. W. and Hunter, J. E. 1995. Conditioning sclerotria of Sclerotinia sclerotiorum for carpogenic germination. Plant Dis. 79: 411 – 415
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This research was conducted at the Faculty of Agriculture, Department of
Food Science laboratories to isolate lactic acid bacteria from some Syrian milk
products (milk, yogurt, cheese) and determine some morphological,
physiological characteristic and the ability of these isolates to ferment sugars.
The antagonistic activity of liquid culture filtrate of Trichodermaharzianum at
different concentrations (5, 10, 20 and 30%) was evaluated "in vitro" at 25 C° against
following plant and human pathogenic fungi: Aspergi
llusniger, Rhizopusstolonifer,
Fusariumoxysporum, F.moniliforme, Alternariaalternata and Candida albicans.
The results showed high level activity of culture filtrate inhibiting the growth of
tested fungi.
The activity was varied with the different species of fungi and the different
concentrations of culture filtrate. The highest activity was against fungus A.niger which
showed precent inhibition equal 96.3% at 30% concentration. Whereas the lowest
inhibition percentage to 77.6% was recorded for fungus A.alternata in comparison with
other fungi.
The culture filterate was not affected the radial colonies growth of R.stolonifer at 5%
and 10% concentrations, however the colonies was fragile and weak, and clearly inhibited
the spores formation.
The culture filtrate was showed high antagonistic capability against the human
pathogenic fungus C.albicans, and the radial inhibition growth was 1.38 cm at 30%
concentration.
أجري هذا البحث في مخابر كلية الزراعة بجامعة الفرات قسم علوم الأغذية وفي مخابر هيئة الطاقة الذرية بدمشق وقد هدف إلى عزل وتعريف بعض سلالات بكتريا حمض اللبن من الحليب الخام للنوق والماعز الشامي بالإضافة إلى دراسة بعض الخصائص الكيميائية والتصنيعية للسلالات المعزولة وذلك في عامي ( 2010و 2011)
Economic losses resulting from the impact of brucellosis on animals have
increased, which is reflected in turn on humans. Brucella is pathogenic bacteria
shared by humans and pet animals. They still pose a significant risk that
threatens health an
d economics in Syria. Many efforts have focused to prevent
the spread of brucellosis disease, by resorting to the use of vaccines prepared
from Brucella strains, where now the target is preparation of vaccine
components proteins of Brucella can be used as a vaccine or an improved
traditional vaccine.
Two bacterial species were isolated from petroleum hydrocarbon-contaminated soil.
The isolates were characterized based on their gram reaction characteristics, morphological
and biochemical properties.
The results showed two bacteria isolated the
first isolated: gram negative short rod,
urease and nitrate reduction test showed a positive result , and glucose test showed positive
result.
And the second isolated: gram positive cocci, gelatin and Citrate utilization test
showed a positive result , and maltose, mannitol test showed negative result.
They were then incubated at (37) ° C for (48- 72) hours.
This is based on comparison of results with Bergey Manual of systematic
bacteriology.
The isolates were identified to be: Pseudomonas pachastrellae (X1)
Micrococcus brunensis (X2).
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