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Register a new userIsolation and selection of superior fungi from fresh olive mill wastewater for biotreatrment
عزل و اختيار أفضل العزلات الفطرية من ماء الجفت الطازج لاستخدامها في المعالجة الحيوية
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Tishreen University ورقة بحثية
Publication date
2015
fields
Chemistry
and research's language is
العربية
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Shamra Editor
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هدف هذه الدراسة هو عزل و توصيف فطريات من ماء جفت مخمر، و تحديد العزلات الأكثر قدرة على احتمال التراكيز العالية للحمولات العضوية و الفينولات الكلية في ماء الجفت الطازج. تم التعرف على ثلاث عزلات تم توصيفها من خلال دراسة الصفات المورفولوجية و البيومترية على وسط الزراعة وسط البطاطا ديكستروز آغار Potato Dextrose Agar (PDA)، و وسط تشابك آغار Czapak Dox Agar (CzA)، و هي عزلة من النوع Aspergillus sclerotiorum و عزلة من النوع Paecilomyces niveus، و عزلة من النوع Mucor nircinelloides. و في اختبار عزلات الأنواع الثلاث على ثلاثة أوساط اختبارية استخدم فيها ماء الجفت (Olive Mill Wastewater) الطازج بتراكيز OMWA3 (100%OMW) ،OMWA2 (75%OMW) و OMWA1 (50%OMW)، تبين أن العزلة من النوع Paecilomyces niveus هي الأكثر قدرة على احتمال تراكيز عالية من حمولات ماء الجفت الطازج.
The aim behind this study was to isolate and characterize fungi from fermented olive mill wastewater to determine the superior one that can resist high organic load and total phenol of fresh Olive Mill Wastewater (OMW). Two different media Czapak Dox Agar (CzA) and Potato Dextrose Agar (PDA) were used for identification of fungi by studying macroscopic and microscopic characteristics. However, Aspergilluss sclerotiorum, Paecilomyces sniveus and Mucor nircinelloides had had been identified . After testing the isolates on fresh olive mill wastewater solid mediums OMWA1 (50%OMW), OMWA2 (75%OMW) and OMWA3 (100%OMW). Whereas P. niveus proves that was the superior one, which can resist high concentration of fresh olive mill wastewater.
References used
PARASKEVA, P.; DIAMADOPOULOUS, E .Review Technologies for Olive Mill Wastewater OMW Treatment: A Review .Journal of Chemical Technology and Biotechnology ,Vol. 81, 2006 ,1475-1485
ALBURQUERQUE, A.; GONZALVEZ, J.; GARCIA, D.; CEGARRA, J. Agrochemical Characterization of “Alperujo” A Solid By-Product of the Two Centrifugation Method for Olive Oil Extraction. Bioresource Technology, Vol. 91, No. 2, 2004, 195-200
ZORPAS, A.; COST, A. Combination of Fenton oxidation and composting for the treatment of the olive solid residue and the olive mile wastewater from the olive oil industry in Cyprus Bioresour. Bioresource Technology, Vol. 101, 2010, 7984–7987
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The Mediterranean region is world’s leading olive growing area. A large amount of
liquid waste results from olive oil extraction these wastewaters have a different effects an
environmental . In this study, four fungal isolates from Samples of soil
Polluted by olive
mill wastewater ,this isolates follow to several types from deuteromycetes, they are follow
to this types : Humicola sp., paecilomyces javanicus, Aspergillus terreus, Aspergillus
flavus. They isolated them on tow media (PDA, CzA) and identify them on colony
character ,Biometric and compare them with reference studies.
3,4-dihydroxybenzoic acid is one of phenolic compounds that exists in olive mill waste water (OMWW) and different industrial wastewater.Commercially activated charcoal was studied as absorbent of polyphenols. The value of specific surface area was 73
6.7189 m2/g. Equilibriums of 3,4-dihyroxybenzoic acid from aqueous solutions on four different particle sizes of the adsorbent with diameters ranged A(1000-2000), B(500-1000), C(250-500), D(125-250)m was examined at different initial concentrations ofabsorbate. The experimental data were analyzed using the Langmuir and Freundlich isotherm models. Both the isotherm models fit the adsorption data. Monolayer absorption capacities Q0 calculated from Langmuir equation were (0.36, 0.23, 0.34, 0.35mmol/g) for the different granular activated carbon sizes (A,B,C,D) respectively. The values of constant Kf calculated from Freundlich equation were (0.75, 0.61, 0.76, 0.79mmol/g) for the four sizes of granular activated carbon (A,B,C,D) respectively.
The results show that the charcoal activated carbon can be used as absorbent for polyphenol compounds.
أجري البحث في مزرعة كلية الزراعة بجامعة دمشق عام 2017 . حيث تمت زراعة نبات القصب في أحواض بمعدل 4 شتول في الحوض الواحد وسقي بماء الجفت بتراكيز مختلفة
A total of 30 water samples and 45 infected fish (carp, Cyprinus carpio) were
collected from freshwater fish farm (Dam of 16 Tishreen-Lattakia) and
analysed bacteriological. Macroscopic examinations of infected fish had showed
the presence of haem
orrhagic skin lesions with brown or red spots throughout
their skin. A total of 64 Aeromonas strains were isolated. The Aeromonas
isolates were distributed as follows: Aeromonas hydrophila (34, 53%), A. caviae
(16, 25%), A. sobria (9, 14%) and (5, 8%) of unidentified aeromonads.
Collectively, Aeromonas spp. are considered as opportunistic causative agents
of human gastroenteritis and other infections. Antibiotic susceptibility tests
were carried on all strains of isolated Aeromonas spp. using twenty different
antibiotics by agar disk diffusion method.
In this study, we review the possibility of production both ethanol and methanol from
OMW(OMW: Olive Mill Wastewater), fermented using pathogenic bacteria and isolated
marine bacteria. The results showed that when using Salmonella sp. bacteria, the
highest
values of methanol (5658.308 μg /l) appear at 7.5% concentration of the OMW, and the
higher concentration of ethanol (49.132 μg/l) at a concentration of 17.5% fermentation of
OMW. While Pseudomonas sp. produced the highest concentration of methanol (603.76μ
g/l) and ethanol (688.71μg /l) at 17.5% for OMW.
The results of this research showedthe ability of the bacteria isolated from sea water
in producing the above mentioned alcohols; for example the X3 bacteria displayed a direct
correlation between high concentrations of OMW (15 , 35 , 50)% and the high
concentrations of produced methanol (UL, 130.406μg/l, 1353.244μg/l ), apposite to
ethanol which was under the detection limits (under limited: UL).
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