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Isolation and selection of superior fungi from fresh olive mill wastewater for biotreatrment

عزل و اختيار أفضل العزلات الفطرية من ماء الجفت الطازج لاستخدامها في المعالجة الحيوية

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 Publication date 2015
  fields Chemistry
and research's language is العربية
 Created by Shamra Editor




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The aim behind this study was to isolate and characterize fungi from fermented olive mill wastewater to determine the superior one that can resist high organic load and total phenol of fresh Olive Mill Wastewater (OMW). Two different media Czapak Dox Agar (CzA) and Potato Dextrose Agar (PDA) were used for identification of fungi by studying macroscopic and microscopic characteristics. However, Aspergilluss sclerotiorum, Paecilomyces sniveus and Mucor nircinelloides had had been identified . After testing the isolates on fresh olive mill wastewater solid mediums OMWA1 (50%OMW), OMWA2 (75%OMW) and OMWA3 (100%OMW). Whereas P. niveus proves that was the superior one, which can resist high concentration of fresh olive mill wastewater.

References used
PARASKEVA, P.; DIAMADOPOULOUS, E .Review Technologies for Olive Mill Wastewater OMW Treatment: A Review .Journal of Chemical Technology and Biotechnology ,Vol. 81, 2006 ,1475-1485
ALBURQUERQUE, A.; GONZALVEZ, J.; GARCIA, D.; CEGARRA, J. Agrochemical Characterization of “Alperujo” A Solid By-Product of the Two Centrifugation Method for Olive Oil Extraction. Bioresource Technology, Vol. 91, No. 2, 2004, 195-200
ZORPAS, A.; COST, A. Combination of Fenton oxidation and composting for the treatment of the olive solid residue and the olive mile wastewater from the olive oil industry in Cyprus Bioresour. Bioresource Technology, Vol. 101, 2010, 7984–7987
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The Mediterranean region is world’s leading olive growing area. A large amount of liquid waste results from olive oil extraction these wastewaters have a different effects an environmental . In this study, four fungal isolates from Samples of soil Polluted by olive mill wastewater ,this isolates follow to several types from deuteromycetes, they are follow to this types : Humicola sp., paecilomyces javanicus, Aspergillus terreus, Aspergillus flavus. They isolated them on tow media (PDA, CzA) and identify them on colony character ,Biometric and compare them with reference studies.
3,4-dihydroxybenzoic acid is one of phenolic compounds that exists in olive mill waste water (OMWW) and different industrial wastewater.Commercially activated charcoal was studied as absorbent of polyphenols. The value of specific surface area was 73 6.7189 m2/g. Equilibriums of 3,4-dihyroxybenzoic acid from aqueous solutions on four different particle sizes of the adsorbent with diameters ranged A(1000-2000), B(500-1000), C(250-500), D(125-250)m was examined at different initial concentrations ofabsorbate. The experimental data were analyzed using the Langmuir and Freundlich isotherm models. Both the isotherm models fit the adsorption data. Monolayer absorption capacities Q0 calculated from Langmuir equation were (0.36, 0.23, 0.34, 0.35mmol/g) for the different granular activated carbon sizes (A,B,C,D) respectively. The values of constant Kf calculated from Freundlich equation were (0.75, 0.61, 0.76, 0.79mmol/g) for the four sizes of granular activated carbon (A,B,C,D) respectively. The results show that the charcoal activated carbon can be used as absorbent for polyphenol compounds.
أجري البحث في مزرعة كلية الزراعة بجامعة دمشق عام 2017 . حيث تمت زراعة نبات القصب في أحواض بمعدل 4 شتول في الحوض الواحد وسقي بماء الجفت بتراكيز مختلفة
A total of 30 water samples and 45 infected fish (carp, Cyprinus carpio) were collected from freshwater fish farm (Dam of 16 Tishreen-Lattakia) and analysed bacteriological. Macroscopic examinations of infected fish had showed the presence of haem orrhagic skin lesions with brown or red spots throughout their skin. A total of 64 Aeromonas strains were isolated. The Aeromonas isolates were distributed as follows: Aeromonas hydrophila (34, 53%), A. caviae (16, 25%), A. sobria (9, 14%) and (5, 8%) of unidentified aeromonads. Collectively, Aeromonas spp. are considered as opportunistic causative agents of human gastroenteritis and other infections. Antibiotic susceptibility tests were carried on all strains of isolated Aeromonas spp. using twenty different antibiotics by agar disk diffusion method.
In this study, we review the possibility of production both ethanol and methanol from OMW(OMW: Olive Mill Wastewater), fermented using pathogenic bacteria and isolated marine bacteria. The results showed that when using Salmonella sp. bacteria, the highest values of methanol (5658.308 μg /l) appear at 7.5% concentration of the OMW, and the higher concentration of ethanol (49.132 μg/l) at a concentration of 17.5% fermentation of OMW. While Pseudomonas sp. produced the highest concentration of methanol (603.76μ g/l) and ethanol (688.71μg /l) at 17.5% for OMW. The results of this research showedthe ability of the bacteria isolated from sea water in producing the above mentioned alcohols; for example the X3 bacteria displayed a direct correlation between high concentrations of OMW (15 , 35 , 50)% and the high concentrations of produced methanol (UL, 130.406μg/l, 1353.244μg/l ), apposite to ethanol which was under the detection limits (under limited: UL).
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