The Bio-mass was isolated from the medium of the water olive
soaking , and was developed on two medium olive mill wastewater
OMW.
The aim behind this study was to isolate and characterize fungi from fermented olive
mill wastewater to determine the superior one that can resist high organic load and total
phenol of fresh Olive Mill Wastewater (OMW).
Two different media Czapak
Dox Agar (CzA) and Potato Dextrose Agar (PDA) were
used for identification of fungi by studying macroscopic and microscopic characteristics.
However, Aspergilluss sclerotiorum, Paecilomyces sniveus and Mucor nircinelloides had
had been identified .
After testing the isolates on fresh olive mill wastewater solid mediums OMWA1
(50%OMW), OMWA2 (75%OMW) and OMWA3 (100%OMW).
Whereas P. niveus proves that was the superior one, which can resist high
concentration of fresh olive mill wastewater.
In this study, we review the possibility of production both ethanol and methanol from
OMW(OMW: Olive Mill Wastewater), fermented using pathogenic bacteria and isolated
marine bacteria. The results showed that when using Salmonella sp. bacteria, the
highest
values of methanol (5658.308 μg /l) appear at 7.5% concentration of the OMW, and the
higher concentration of ethanol (49.132 μg/l) at a concentration of 17.5% fermentation of
OMW. While Pseudomonas sp. produced the highest concentration of methanol (603.76μ
g/l) and ethanol (688.71μg /l) at 17.5% for OMW.
The results of this research showedthe ability of the bacteria isolated from sea water
in producing the above mentioned alcohols; for example the X3 bacteria displayed a direct
correlation between high concentrations of OMW (15 , 35 , 50)% and the high
concentrations of produced methanol (UL, 130.406μg/l, 1353.244μg/l ), apposite to
ethanol which was under the detection limits (under limited: UL).