Effect of different degrees of temperature, different levels of pH , different periods of
light and four media (PDA, OMA, MEA, CzA) was studied in vitro against the growth of
C. gloeosporioides causing anthracnose disease of citrus fruits. The resu
lts indicated that
the growth of fungus was maximum at temperature 30°C and at pH = (5.5,6,6.5), and Light
was unnecessary for the growth of C. gloeosporioides. Among the different media tested
PDA and OMA supported the maximum growth of fungus.
The results of this study showed the important role of the light
intensity compared with the light exposure time on coloring the new
formed leaves, also this study had showed that the leaves are not
affected by the different temperatures, and also
that these plants showed
a decrease in the different pigment substances in close proportions.
Concerning the amount of proteins, the results have shown main
differences in the protein bands between the mutated and the natural
species, but we have found a decrease in the amount of proteins in the
mutated species than in natural species.