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Effect of different degrees of temperature, different levels of pH , different periods of light and four media (PDA, OMA, MEA, CzA) was studied in vitro against the growth of C. gloeosporioides causing anthracnose disease of citrus fruits. The resu lts indicated that the growth of fungus was maximum at temperature 30°C and at pH = (5.5,6,6.5), and Light was unnecessary for the growth of C. gloeosporioides. Among the different media tested PDA and OMA supported the maximum growth of fungus.
The results of this study showed the important role of the light intensity compared with the light exposure time on coloring the new formed leaves, also this study had showed that the leaves are not affected by the different temperatures, and also that these plants showed a decrease in the different pigment substances in close proportions. Concerning the amount of proteins, the results have shown main differences in the protein bands between the mutated and the natural species, but we have found a decrease in the amount of proteins in the mutated species than in natural species.
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