In September-october 2014 the collected air green samples of
zizuphus jujuba was extracted .Suitable conditions were chosen to
separate the components of the volatile oil by the components of the
volatile oil by Gs/Ms and we noticed.
- 16 compoun
ds were found in the extracted volatile oil of the
extraction by petrolium ether, the main compound is palmetoleic acid ,
it form 29.13% of the volatile oil weight , and palmitic acid, it form
15.5% .Also volatile oil of the extracted from fresh leaves by steam
distillation contain 24 compounds, the main compound is Oleamid ,it
from 23.30% of the volatile oil weight.
Species of the genus Campylobacterare recognized as the main cause of foodborne disease in both developing and developed countries. They are main causative of diarrhea and gastroenteritis worldwide. Staphylococcus bacteria especially S. aureus are re
sponsible of food poisoning due to their ability to produce enterotoxins.
Traditional methods which are used to identify bacteria are time-consuming and labor intensive and very expensive.
Thirteen isolates of Campylobacter and twenty-two isolates of Staphylococcus were isolated from a variety of foods. Traditional methods based on biochemical tests were used for identification in addition to laser light-scattering technique to discriminate isolates.
Laser light-scattering technique showed the ability to distinguish all isolate in high accuracy, rapid and low costs manner, thus it may represent a potential alternative to traditional methods to identify these bacteria.
So it is a very important issue to find new alternative methods characterized by high accuracy, low costs and rapidity in results achievement, to replace traditional methods, thus laser light-scattering may be a possible alternative
This research aimed to study the effect of various growth conditions
(temperature, culture age and culture medium) on the fatty acids composition
of cytoplasm membrane of 37 isolates of Staphylococcus aureus using Gas
Chromatography-Mass Spectrome
try (GC-MS). Results revealed that the total
number of fatty acids of Staph. aureus was 33. Eight of them were principle and
included: anti-isotetradecanoic acid (aC15:0), octadecanoic acid (C18:0),
eicosanoic acid (C20:0), isotetradecanoic acid (Ci14:0), isotridecanoic acid
(Ci15:0), hexadecanoic acid (C16:0), antisohexa- decanoic acid (Ca17:0) and
octadecenoic acid (C18:1) with a percentage of 33.96, 18.37, 14.94, 7.32, 6.23,
4.14, 3.03 and 2.89%, respectively. The percentage of each of the other fatty
acids was less than 1%. Increasing the incubation temperature was associated
with reducing the percentages of Ca15:0, Ci15:0, C16:0 and C18:1; and
increasing Ca17:0, Ci14:0, C18:0 and C20:0. The increase in incubation period
caused a decrease in C16:0, C18:0, C18:1 and C20:0; and an increase in Ca15:0
and Ca17:0. Moreover, the variation in culture media caused differences in the
percent of Ca15:0, C18:0 and C20:0; while the other fatty acids showed no
changes.
This study was carried out to detect the presence of aero-mesophyllic
microorganisms and the patho-toxicogenic microorganisms in the consumed
variety meats of Awassi-sheep carcasses.
Surface cut samples of the tested organs with ١٢ cm٢ and ٢ mm in
thickness,
were taken immediately from animals slaughtered in the slaughterhouse or in
the commercial public market, where these consumable variety meats are sold.
