Effect of different degrees of temperature, different levels of pH , different periods of
light and four media (PDA, OMA, MEA, CzA) was studied in vitro against the growth of
C. gloeosporioides causing anthracnose disease of citrus fruits. The resu
lts indicated that
the growth of fungus was maximum at temperature 30°C and at pH = (5.5,6,6.5), and Light
was unnecessary for the growth of C. gloeosporioides. Among the different media tested
PDA and OMA supported the maximum growth of fungus.
Fusarium oxysporum and F. solani, causal agents of wilt and rot root in citrus
seedling are variable pathogens. This study was conducted on the effect of different media
in characters, sporulation, and spore germination of fusarium spp. Isolates th
at were
isolated from seed and seedling of citrus.
The data revealed that the maximum mycelial growth was obtained in most isolates
on Potato Sucrose agar for F. oxysporum and F. solani, followed by Potato Dextrose agar
for F. oxysporum, and by Czapek’sDox agar for F. solani. The maximum sporulation was
recorded in Potato Sucrose agar medium followed byCzapek’sDox agar .
The study also showed that Potato Dextrose agar was the best for germination of
spores.
