This study evaluated the in vitro
antileishmanial activity of some Phenolic compounds including:
Ferulic acid, Mehtyl Ferulate, Ethyl Ferulate, and Sinapic acid
against leishmanial tropica promastigotes. The parasite viability
was determined using XTT proliferation Kit.
The leishmaniasis is a serious health problem in Syria, due to the
wide spreading of Leishmania tropica parasite , the difficulty of
controlling the reservoirs of parasites, and there is no specific ,safe
and active therapy .Due to lack of studies
of Leishmania tropica
genome , as initial step we detect the presence of SW3 gene.
In our study, the
presence and expression of P27 gene in L.tropica evaluated in
promastigotes by PCR and RT-PCR using specific primer pairs
that manually designed after determine the consensus sequence of
forward and reverse region of primers bet
ween species of
Leishmania by using MUSCLE (Multiple Sequence Comparison by
Log- Expectation) tool for alignment. The results proved the
presence of P27 gene in Leishmania tropica, beside presence the
mRNA of P27 gene that confirm that P27 expressed in
promastigote form of L.tropica
DNA was extraction from scanning microscopic slides to
identification leishmaniases. Three methods used to DNA
extraction, first one using ready extraction solvent (Promega kit),
second depended on phenol-chloroform-iso propanol , whereas third
one depended on columns with silica gel membranes (Qia gen kit).
The results showed that (Promega kit) method didn’t gave high
concentration of DNA. (Qia gen kit) method was the best for DNA
concentration and purity
the leishmaniasis is a serious health problem in Syria, due to the
wide spreading of Leishmania Tropica parasite , the hardness of
controlling the reservoirs of parasites, and there is no specific ,safe
active therapy. A Heat shock protein 20 HSP2
0 belongs to the
small HSP (sHSP) family.
The Syrian flora contains several species belonging to the genus Inula, but, to our knowledge, no integrated scientific study has been published on the types of the species and the type of glutinous species in particular. Therefore, it was necessary to shed light on this important species spread in the Syrian environment.
Cutaneous Leishmaniasis (CL) is one of the most prevalent
clinical forms of leishmaniasis , also it is endemic disease in Syria. And in
spite of the great efforts to control the disease the annual incidence is still
increased. Furthermore, tourism
, migration, and prevalence of acquired
immunodeficiency (AIDS) all these factors increase the spread of this
disease to new areas around the world.
Recently, studies suggest that cytokines gene polymorphisms can contribute
to resistance or susceptibility to many diseases and one of these diseases is
CL .
In the present study, we tried to compare the sensitivity and the specificity
of the rK39 strips and DAT, to serodiagnose the visceral leishmaniasis disease
in some endemic villages in south of Syria, in order to apply the best and the
easy test i
n the epidemiological studies, for serodiagnosis this disease not only in
symptomatic patients but also in asymptomatic and suspected cases in order to
treat them early and rapidly.
According to the high specificity and sensitivity of ELISA test, we tried in
this preliminary study, to certify the usefulness of ELISA test in the detection of
specific antibodies (IgG) to cutaneous leishmaniasis(CL) in SYRIA, in order to
certify
the diagnosis of this disease by using the classic methods (observation
microscopic – culture).
In this study the total of ٤١٥ cases of cutaneous leishmaniasis was evaluated
from Dermatology hospital and General clinic in Damascus from February
٢٠٠١ to February ٢٠٠٢. Most of these cases was reported in Damascus and its
suburb, and when we ma
de the direct microscopic test, amastigotes were
detectable in ٢٧١ cases, and the positive cases was increased to ٣٦٤ cases by
realizing the parasite culture into diphasic N. N. N. media, which confirm the
diagnosis.
