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Register a new userPreliminary Serodiagnosis study to certify the diagnosis of cutaneous leishmaniasis in Syria
دراسة تشخيصية مصلية أولية لدعم الطرائق التقليدية للكشف عن داء الليشمانية الجلدي في سورية
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حاولنا من خلال هذه الدراسة الأولية في سورية، تسليط الضوء علـى إمكانيـة اسـتخدام اختبـار المقايسة المناعية الأنزيمية (ELISA) في الكشف عن أضداد الليشمانية الجلدية في مصول المصابين أو المشكوك بإصابتهم لدعم الطرائق التقليدية (فحص مجهري مباشر و استنبات) المستخدمة فـي تـشخيص الإصابة بداء الليشمانية الجلدي نظراً لأن حساسية هذا الاختبار و نوعيته مرتفعتان. فلدى إجراء اختبـار الإليزا على 270 حالة مأخوذة من مشفى الأمراض الجلدية و الزهرية – جامعة دمشق، حصلنا على 250 عينة إيجابية أي بنسبة 5.92 % بالمقارنة مع عدد العينات الإيجابية التي تم تشخيصها بالطرائق التقليدية و التي بلغ عددها 225 حالة أي بنسبة 3.83 % في حين كانت نتيجة اختبار الإليزا المطبق على المصول الشاهدة (20 مصلاً) سالبة.
According to the high specificity and sensitivity of ELISA test, we tried in this preliminary study, to certify the usefulness of ELISA test in the detection of specific antibodies (IgG) to cutaneous leishmaniasis(CL) in SYRIA, in order to certify the diagnosis of this disease by using the classic methods (observation microscopic – culture).
References used
(World Health Organization. Leishmania & HIV in Gridlock. WHO and Joint UN programme, (1998
Mathis A. and Deplazes P. PCR and in vitro cultivation for detection of Leishmania spp. In diagnostic samples from humans and dogs. J. Clin. Microbiol. (1995) 33: 1145-1149
(Shabaan M., Hammoud L., and Nahhas S. Epidemiological of Cutaneous Leishmaniasis of cases from dermatology hospital and general clinic in Damascus. Damas. Univ. J. Bas. Sci. (2003
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In this study the total of ٤١٥ cases of cutaneous leishmaniasis was evaluated
from Dermatology hospital and General clinic in Damascus from February
٢٠٠١ to February ٢٠٠٢. Most of these cases was reported in Damascus and its
suburb, and when we ma
de the direct microscopic test, amastigotes were
detectable in ٢٧١ cases, and the positive cases was increased to ٣٦٤ cases by
realizing the parasite culture into diphasic N. N. N. media, which confirm the
diagnosis.
Through this research, case studies were reviewed which reviewed the dermatology department at Tishreen University Hospital in Lattakia and specialized centers for leishmaniasis and parasitic diseases in Lattakia and Tartous governorates from the beg
inning of April 2018 until the end of December 2019. Where Syria is one of the areas endemic with cutaneous leishmaniasis, as we have noticed in recent years the number of skin infections has increased dramatically in all Syrian governorates, which has made leishmaniasis an important health problem that warrants study. In order to isolate and determine the type and strains of the parasite responsible for skin injuries in Syria, in order to develop a plan to combat this disease and limit its spread. Microscopy and transplantation methods are the gold standard for diagnosing cutaneous leishmaniasis, especially in poor areas where this disease is common. Through this study it was found that the age group most exposed to infection is between 16 - 40 years and that the highest incidence was among males. As for the distribution of skin lesions, the highest percentage was recorded on the face, upper and lower extremities.
In the present study, we tried to compare the sensitivity and the specificity
of the rK39 strips and DAT, to serodiagnose the visceral leishmaniasis disease
in some endemic villages in south of Syria, in order to apply the best and the
easy test i
n the epidemiological studies, for serodiagnosis this disease not only in
symptomatic patients but also in asymptomatic and suspected cases in order to
treat them early and rapidly.
The diagnosis of visceral leishmaniasis (VL) remains problematic for
physicians in Syria, because the appearance of this disease is new and its
clinical criteria are similar to Malaria, Tuberculosis, and Toxoplasmosis
symptoms. For this reason, we
used the specific antigen (rK39) of
Leishmania donovani complex, in order to certify the appearance of
visceral leishmaniasis in some patients. Then we followed the infected
cases with VL during treatment (15 days) and after treatment (10 days)
with Glucantime®. We noted an improvement in clinical criteria and a
significant decrease in the parasite numbers as well as in the specific L.
donovani antibodies.
Patients infected with different species of Leishmania in Syria develop
specific antibodies. For this reason, we have developed an immunodot assay for
the serodiagnosis of cutaneous and visceral leishmaniasis, which utilizes antihuman
IgG conjugat
ed with peroxidase (HRP) as the visualizing agent.
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