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Register a new userDetect The Presence of HSP20 Gene In The Leishmania Tropica Genome
تحري وجود جين بروتين الصدمة الحرارية 20 HSP20 في جينوم الليشمانية المدارية
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يشكل داء الليشمانيات مشكلة صحية هامة في سوريا, نظرا للانتشار الواسع لطفيليات الليشمانية المدارية, و صعوبة السيطرة على مخازن الطفيلي, و كذلك عدم وجود أدوية فعالة و آمنة في آن معاً. و نظرا لقلة الدراسات التي تتناول جينوم الليشمانية المدارية, قمنا في هذه الدراسة بتحري وجود جين بروتين الصدمة الحرارية 20 Heat shock) hsp20) protein 20 في جينوم طفيلي الليشمانية المدارية.
the leishmaniasis is a serious health problem in Syria, due to the wide spreading of Leishmania Tropica parasite , the hardness of controlling the reservoirs of parasites, and there is no specific ,safe active therapy. A Heat shock protein 20 HSP20 belongs to the small HSP (sHSP) family.
References used
Ready PD. "Leishmaniasis emergence in Europe". Euro Surveill. 2010;15(10):pii=19505
Alam E1, Abbas O2, Moukarbel R1 and Khalifeh I3." Cutaneous Leishmaniasis: An Overlooked Etiology of Midfacial Destructive Lesions". PLoS Negl Trop Dis. 2016 Feb 10;10(2):e0004426
Cox F, Wakelin D and Kreier J." Old World leishmaniasis" in Topley and Wilson's Microbiology and Microbial Infections: Parasitology 10e.ed. Kreier J.pub. Arnold E,2006,754-840
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The leishmaniasis is a serious health problem in Syria, due to the
wide spreading of Leishmania tropica parasite , the difficulty of
controlling the reservoirs of parasites, and there is no specific ,safe
and active therapy .Due to lack of studies
of Leishmania tropica
genome , as initial step we detect the presence of SW3 gene.
In our study, the
presence and expression of P27 gene in L.tropica evaluated in
promastigotes by PCR and RT-PCR using specific primer pairs
that manually designed after determine the consensus sequence of
forward and reverse region of primers bet
ween species of
Leishmania by using MUSCLE (Multiple Sequence Comparison by
Log- Expectation) tool for alignment. The results proved the
presence of P27 gene in Leishmania tropica, beside presence the
mRNA of P27 gene that confirm that P27 expressed in
promastigote form of L.tropica
Kinetoplastid membrane protein-11 (KMP-11), a protein present in all kinetoplastid
protozoa studied up to date, is considered a potential vaccine candidate for Leishmaniasis.
Such vaccine molecules must be expressed in amastigotes which represent t
he infective
forms for mammals, while promastigotes are the flagellate forms found in the insect hosts.
However, the expression of KMP-11 in amastigotes is still a subject of controversy. In this
study, a strain of L. tropica was isolated, cultivated, and genotyped. The expression of
KMP-11 gene in this strain was evaluated in promastigotes and in amastigotes by RT-PCR
using specific primer pairs. The results proved the presence of mRNA of KMP-11 in both
promastigotes and amastigotes forms of L. tropica. The expression of this molecule in
amastigotes is consistent with the previously demonstrated immunoprotective capacity of
KMP-11 DNA vaccine as well as the presence of humoral and cellular immune responses
against KMP-11 in Leishmania-infected animals.
This study evaluated the in vitro
antileishmanial activity of some Phenolic compounds including:
Ferulic acid, Mehtyl Ferulate, Ethyl Ferulate, and Sinapic acid
against leishmanial tropica promastigotes. The parasite viability
was determined using XTT proliferation Kit.
The Syrian flora contains several species belonging to the genus Inula, but, to our knowledge, no integrated scientific study has been published on the types of the species and the type of glutinous species in particular. Therefore, it was necessary to shed light on this important species spread in the Syrian environment.
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