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Register a new userEffect of Sinapic acid and Ferulic acid and its Derivatives on viability of Leishmania tropica promastigotes
تقييم الفعالية المضادة لمشيقات الليشمانية المدارية في الزجاج لكل من حمض السينابيك و مشتقات حمض الفيروليك
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Aِl-Baath University ورقة بحثية
Publication date
2018
fields
Pharmacy
and research's language is
العربية
Created by
Shamra Editor
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تهدف هذه الدراسة إلى تقييم فعالية حمض الفيروليك Ferulic acid و الميتيل فيرولات Methyl Ferulate، و الايثيل فيرولات Ethyl Ferulate و حمض السينابيك Sinapic acidعلى تكاثر مشيقات Promastigote المسببة لداء الليشمانية الجلدي.
This study evaluated the in vitro antileishmanial activity of some Phenolic compounds including: Ferulic acid, Mehtyl Ferulate, Ethyl Ferulate, and Sinapic acid against leishmanial tropica promastigotes. The parasite viability was determined using XTT proliferation Kit.
References used
Al-Natour,S. 2009, UPDATE IN THE TREATMENT OF CUTANEOUS LEISHMANIASIS. J Family Community Med . 16(2):41-47
Al-Salem,W. 2016, Cutaneous Leishmaniasis and Conflict in Syria. Emerging Infectious Diseases. 22(5):931-933. doi:/10.3201/eid2205.160042
Alvar, J. Velez, I. Bern, C. Herrero M. Desjeux, PH. Cano, J. Jannin, J. Den boer, M. 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS One.7(5): e35671
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A partial purification was performed for the fungal hydroxycinnamic acid
esterase (HCAE) from Humicola sp. by precipitation using either ammonium
sulfate or acetone.
In our study, the
presence and expression of P27 gene in L.tropica evaluated in
promastigotes by PCR and RT-PCR using specific primer pairs
that manually designed after determine the consensus sequence of
forward and reverse region of primers bet
ween species of
Leishmania by using MUSCLE (Multiple Sequence Comparison by
Log- Expectation) tool for alignment. The results proved the
presence of P27 gene in Leishmania tropica, beside presence the
mRNA of P27 gene that confirm that P27 expressed in
promastigote form of L.tropica
Total of 33 isolates of lactic acid bacteria from different
samples (Dairy products, Pickles), were isolated and identified
according to the common characteristics of lactic acid bacteria.
Twinty-five isolates were rods and 9 were cocci.
The leishmaniasis is a serious health problem in Syria, due to the
wide spreading of Leishmania tropica parasite , the difficulty of
controlling the reservoirs of parasites, and there is no specific ,safe
and active therapy .Due to lack of studies
of Leishmania tropica
genome , as initial step we detect the presence of SW3 gene.
Kinetoplastid membrane protein-11 (KMP-11), a protein present in all kinetoplastid
protozoa studied up to date, is considered a potential vaccine candidate for Leishmaniasis.
Such vaccine molecules must be expressed in amastigotes which represent t
he infective
forms for mammals, while promastigotes are the flagellate forms found in the insect hosts.
However, the expression of KMP-11 in amastigotes is still a subject of controversy. In this
study, a strain of L. tropica was isolated, cultivated, and genotyped. The expression of
KMP-11 gene in this strain was evaluated in promastigotes and in amastigotes by RT-PCR
using specific primer pairs. The results proved the presence of mRNA of KMP-11 in both
promastigotes and amastigotes forms of L. tropica. The expression of this molecule in
amastigotes is consistent with the previously demonstrated immunoprotective capacity of
KMP-11 DNA vaccine as well as the presence of humoral and cellular immune responses
against KMP-11 in Leishmania-infected animals.
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