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Register a new usermolecular characterization for Some Local and Introduced Grape Cultivars Vitis vinifera .L in Sweida Governorate Using SSR Technique
التوصيف الجزيئي لبعض أصناف العنب Vitis vinifera .L المحلية و المدخلة في محافظة السويداء باستخدام تقنية SSR
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نفذ البحث في مخابر هيئة الطاقة الذرية قسم البيولوجيا الجزيئية و التقانة الحيوية بدمشق, و تمت الدراسة على خمسة أصناف من العنب المحلية و المدخلة أخذت من مركز بحوث التفاحيات والكرمة في السويداء بهدف توصيفها جزيئياً و تحديد درجة القرابة الوراثية بينها, باستخدا تقنية ال SSR و استخدم لذلك 20 زوجاً من البادئات المتخصصة.
This research was carried out in the laboratories of the Atomic Energy Commission Molecular Biology and Biotechnology Department in Damascus to study the molecular characterization of five of local and introduced grapes varieties which collected from the Pome and Grapevine Research Center in Sweida using 20 primer pairs of SSR.
References used
Seyedi, A.; Esmaeili, A. and Mostafavi, M. (2013)." Study of stem type and different levels of IBA on rooting of Bougainvillea glabra". International Journal of Agriculture and Crop Sciences, 5(12): 1276−1279
Ricciardi, L.; Giorgio, V.; Giovanni, C. De.; Lotti, C.; Gallotta, A. and Fanizza, G.(2002). "The genetic of apulian apricot genotypes (Prunus armeniaca L.) assessed using AFLP markers". Cellular and Molecular Biology Letters.7:431-436
Semagn, K. ; Bjornstad, A. and Ndjiondjop, M.N. (2006). "An overview of molecular marker methods for plants". African Journal of Biotechnology. 25(5): 2540-2568
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The research was executed in Biotechnology Lab (Faculty of
Agriculture – Damascus University), during the season 2016.
Sixteen genotypes of Sunflower Oil were planted to study the
genetic diversity among these genotypes and to determine the
degree of genetic Agreement using the technique SSR (Simple
Sequence Repeats).
The objective of this study was to characterize and determine the genetic
variation among twelve cultivars and four rootstocks, belonging to Amygdalus
genus in Syria using the simple sequence repeats (SSRs) marker. It was found
that 154 alleles we
re identified by using 26 primer pairs, and the proportion of
the specific ones was 30.52%. In addition, it was revealed that all the studied
SSR loci produced polymorphic alleles. The average of PIC, He and Ho were
0.58, 0.61, 0.31, respectively.
20 local and introduced varieties of pear grown in Sweida during 2007 to
2010 were assessed in terms of flowering time, maturity time, accurate
morphological characterization for shoots, leaves, flowers and fruits and fruit
chemical analysis (tota
l soluble solids, total sugar and tetratable acidity) as well
as to determine firmness of fruit flesh and tree yield.
Results showed that flowering time was at mid April in most varieties, and
the varieties were divided into three groups according to their mature date:
early varieties maturated during July, mid varieties maturated during August
and late varieties maturated during September. However, A variety of
Conference was required artificial ripening.
The wide geographical spread and the exponential growth of the numbers of goats
around the world clearly demonstrate the ability of these ruminants to adapt to
harsh climates and grazing land. The aim of this study was to assess the genetic
divers
ity of 42 samples of Syrian goats from many domestication stations including
Jabali, Shami and hybrid (hybridization between both Shami and Jabali). The study
was done by DNA extraction of these samples, application of SSR technology using
7 microstellite markers. The alleles number of markers were 29 alleles, at a rate of
4.1 allele for each genetic locus. The number of alleles of each locus ranged from 3
alleles in genetically markers (BMS1714, INRAD07, SRCRSP09) to 8 alleles at the
genetic marker SRCRSP01. The rate of allele-frequency across all studied genetic
sites ranged from 0.071429 with the molecular size of 210 bp for the McM527
genetic site to 0.97619 with a molecular size of 55 bp for the SRCRSP09 genetic site.
Morphological and molecular characterization for six seedling genotypes of
Pistachio vera L (V1,V2,V3,V4,V5, and V6) in comparison with the most
commercial and wide spread varieties, Ashoury andWhite batoury was carried
out in Swaida province, sou
thern of Syria. Nuts chemical contents (dry
substance, moisture, total sugar and oil content) for both fresh and dry nuts
weight were also evaluated.
Seedling genotypes showed obvious differences toward the main diagnostic
traits, such as alternate bearing phenomena, the ratio of kernel weight to total
nut weight, split nuts percentage and loss percentage. Genotype V4 was
recognized by some important characters compared with the other genotypes
studied. Cluster analysis was assessed according to the existence or absence of
the most important studying morphological and agronomic indexes. All
genotypes studied and comparative cultivars were clustered into four groups.
RAPD technique was achieved using 25 randomly primers, 19 of which were
polymorphic with an average of polymorphism percentage of (66.47%). The
highest value of genetic similarity (0.81) was between Ashoury and V5
genotype, whereas the lowest value (0.56) was between V3 and V6. Cluster
analysis depending on RAPD data divided the population studied into two main
groups. RAPD unique band was estimated (37 unique band, 21 positive and 16
negative).
It was concluded that RAPD technique can be used efficiently to emphasize,
identify, insure and evaluate the genetic diversity of Pistachio vera.
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