أجريت الدّراسة خلال العام 2016، حيث تضمّنت جمع عيّنات قشور الرز صنف .Oryza sativa L في محافظة الديوانية/العراق، و جُلبت إلى مختبر دائرة البيئة و المياه في وزارة العلوم و التكنولوجيا. نُظّفت، و طحنت، و حُفظت في حاوياتٍ معقمة. تمّ تنمية و عزل عزلة بكتيرية محليّة محلّلة للسيللوز من القشور، بتنميتها على وسط الأملاح المحتوي على السيللوز، و حُضّنت بدرجة حرارة 37°م مدة (24±2) ساعة. شُخّصت العزلة البكتيريّة اعتماداً على المواصفات المظهرية لمستعمرات البكتيرية النامية على وسط الزراعة الصّلب، و مواصفات الفحص المجهري، و بعض الاختبارات البيوكيميائية على أنّها .Bacillus sp.عوملت قشور الرز بعد طحنها كيميائياً بمحلول هيدروكسيد الصوديوم بتركيز1%، ثم عوملت بيولوجياً بتنمية العزلة البكتيريّة في وسط الأملاح المحتوي على قشور الرز، و المعاملة قاعديّاً كمصدر كربوني، و مقارنتها مع العزلة النامية في الوسط المحتوي على السيللوز القياسي، من أجل الاستدلال على المعالجة الحيوية للعزلة البكتيرية. تمّ قياس النمو البكتيري عند طول موجي 600 نانوميتر، فبلغ 0.974 في وسط قشور الرز، بينما في وسط السيللوز القياسي بلغ 0.853 و كذلك تمّ قياس تركيز سكر الغلوكوز، فبلغ 250 ميكرو غرام /مل في وسط قشور الرز، بينما في وسط السيللوز القياسي بلغ 210 ميكرو غرام/مل. بيّنت هذه الدّراسة إمكانيّة التخلص من قشور الرز التي تُعتبر أحد الملوّثات البيئيّة و الاستفادة منها في إنتاج الغلوكوز.
The study was conducted in 2016. The samples of rice Oryza sativa L. straw
were collected from Dewania governorate/Iraq, and were brought to laboratory of
Directorate of Environmental and Water in Ministry of Science and Technology.
The samples were cleaned and milled, then stored in sterile containers. Local
cellulolytic bacterial isolate was cultivated and isolated on mineral and cellulose
medium, at 37 ºC for (24± 2) hours, the bacterial isolate was diagnosed as Bacillus sp.
depending on phenotypes of bacterial colonies on solid medium, microscopic
characters and some biochemical tests. Milled rice straw was chemically treated
with 1% of sodium hydroxide, then biological treatment by bacterial isolate Bacillus sp
cultivate in mineral medium with alkali treated rice straw as carbone source and
compared with cellulose standard medium. The bacterial growth was measured at
600 nm, which reached 0.974 in rice straw medium, while in cellulose medium
reached 0.853. For glucose concentration, the value reached 250 μg/ml in rice straw
medium, while in cellulose medium it was 210 μg/ml. The results concluded the
possibility of getting rid of rice husks, which is an environmental contaminant, and
to use it in the production of glucose.
References used
Abd El-Zaher, F.; and M. Fadel (2010). Production of bioethanol via enzymatic saccharification of rice straw by cellulase produced by Trichoderma reesei under solid state fermentation. New York Sci. J., 3: 72
Abdulla, R.; W. Zafar; M. Nadeem; M. Iqtedar; A. Kaleem and S.H. Naz (2016). Partial purification and characterization of cellulases produced by bacillus strain. Rom. Biotechnol. Lett., 21(1): 11103-11109
Ahmed S.; A. Bashir; H. Saleem; M. Saadia; and A. Jamil (2009). Production and Purification of cellulose degrading enzymes from afilamentous fungus Trichodermaharzianum. Pak. J. Bot., 41(3): 1411-1419
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