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Biological Treatment of Rice Straw and Glucose Production Using Bacillus sp.

المعالجة الحيوية لقشور الرز و إنتاج سكر الغلوكوز باستخدام بكتريا .Bacillus sp

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 Publication date 2018
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and research's language is العربية
 Created by Shamra Editor




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The study was conducted in 2016. The samples of rice Oryza sativa L. straw were collected from Dewania governorate/Iraq, and were brought to laboratory of Directorate of Environmental and Water in Ministry of Science and Technology. The samples were cleaned and milled, then stored in sterile containers. Local cellulolytic bacterial isolate was cultivated and isolated on mineral and cellulose medium, at 37 ºC for (24± 2) hours, the bacterial isolate was diagnosed as Bacillus sp. depending on phenotypes of bacterial colonies on solid medium, microscopic characters and some biochemical tests. Milled rice straw was chemically treated with 1% of sodium hydroxide, then biological treatment by bacterial isolate Bacillus sp cultivate in mineral medium with alkali treated rice straw as carbone source and compared with cellulose standard medium. The bacterial growth was measured at 600 nm, which reached 0.974 in rice straw medium, while in cellulose medium reached 0.853. For glucose concentration, the value reached 250 μg/ml in rice straw medium, while in cellulose medium it was 210 μg/ml. The results concluded the possibility of getting rid of rice husks, which is an environmental contaminant, and to use it in the production of glucose.


Artificial intelligence review:
Research summary
أجريت هذه الدراسة في عام 2016 بهدف معالجة قشور الرز بيولوجياً باستخدام بكتيريا Bacillus sp. تم جمع عينات من قشور الرز من محافظة الديوانية في العراق، وتنظيفها وطحنها وتخزينها في حاويات معقمة. تم عزل بكتيريا محللة للسيللوز من القشور وزراعتها في وسط يحتوي على السيللوز عند درجة حرارة 37 مئوية لمدة 24 ساعة. تم تشخيص العزلة البكتيرية بناءً على الخصائص المظهرية للمستعمرات البكتيرية والاختبارات البيوكيميائية، وتبين أنها من نوع Bacillus sp. تم معالجة قشور الرز كيميائياً باستخدام محلول هيدروكسيد الصوديوم بتركيز 1%، ثم بيولوجياً باستخدام العزلة البكتيرية في وسط يحتوي على قشور الرز المعالجة كيميائياً كمصدر كربوني. تم قياس النمو البكتيري عند طول موجي 600 نانومتر، وبلغ 0.974 في وسط قشور الرز، بينما بلغ 0.853 في وسط السيللوز القياسي. كما تم قياس تركيز سكر الغلوكوز، وبلغ 250 ميكروغرام/مل في وسط قشور الرز، بينما بلغ 210 ميكروغرام/مل في وسط السيللوز القياسي. خلصت الدراسة إلى إمكانية التخلص من قشور الرز التي تعتبر ملوثاً بيئياً والاستفادة منها في إنتاج الغلوكوز.
Critical review
دراسة نقدية: تعد هذه الدراسة خطوة مهمة نحو الاستفادة من المخلفات الزراعية وتحويلها إلى منتجات ذات قيمة اقتصادية. ومع ذلك، يمكن توجيه بعض النقد البنّاء لتحسين البحث في المستقبل. أولاً، لم يتم التطرق إلى تكلفة العمليات الكيميائية والبيولوجية المستخدمة، وهو عامل مهم لتقييم الجدوى الاقتصادية للتطبيق على نطاق واسع. ثانياً، لم يتم مناقشة التأثير البيئي المحتمل للمعالجة الكيميائية باستخدام هيدروكسيد الصوديوم. ثالثاً، كان من الممكن توسيع الدراسة لتشمل أنواع أخرى من المخلفات الزراعية للتحقق من فعالية الطريقة على نطاق أوسع. وأخيراً، كان من الممكن تقديم مزيد من التفاصيل حول خصائص البكتيريا المستخدمة وكيفية تحسين إنتاجيتها.
Questions related to the research
  1. ما هو الهدف الرئيسي من الدراسة؟

    الهدف الرئيسي من الدراسة هو معالجة قشور الرز بيولوجياً باستخدام بكتيريا Bacillus sp لإنتاج سكر الغلوكوز.

  2. ما هي الطريقة الكيميائية المستخدمة لمعالجة قشور الرز؟

    تم معالجة قشور الرز كيميائياً باستخدام محلول هيدروكسيد الصوديوم بتركيز 1%.

  3. ما هو تركيز سكر الغلوكوز الذي تم الحصول عليه في وسط قشور الرز؟

    تم الحصول على تركيز سكر الغلوكوز بلغ 250 ميكروغرام/مل في وسط قشور الرز.

  4. ما هي النتائج التي خلصت إليها الدراسة؟

    خلصت الدراسة إلى إمكانية التخلص من قشور الرز التي تعتبر ملوثاً بيئياً والاستفادة منها في إنتاج الغلوكوز.


References used
Abd El-Zaher, F.; and M. Fadel (2010). Production of bioethanol via enzymatic saccharification of rice straw by cellulase produced by Trichoderma reesei under solid state fermentation. New York Sci. J., 3: 72
Abdulla, R.; W. Zafar; M. Nadeem; M. Iqtedar; A. Kaleem and S.H. Naz (2016). Partial purification and characterization of cellulases produced by bacillus strain. Rom. Biotechnol. Lett., 21(1): 11103-11109
Ahmed S.; A. Bashir; H. Saleem; M. Saadia; and A. Jamil (2009). Production and Purification of cellulose degrading enzymes from afilamentous fungus Trichodermaharzianum. Pak. J. Bot., 41(3): 1411-1419
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