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Register a new userProduction of cellulase enzymes from Bacillus sp bacteria isolated from Local soils and determination of some of thrir molecular properties
إنتاج أنزيمات السيلولاز من بكتريا Bacillus sp المعزولة من الترب المحلية والتعرف على بعض صفاتها الجزيئية
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Damascus University أطروحة دكتوراه
Publication date
2015
fields
Agricultural Engineering
and research's language is
العربية
Created by
Shamra Editor
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جرى في إطار هذا البحث عزل البكتريا التابعة للجنس Bacillus من بعض الترب السورية في محافظة دمشق وريفها ومحافظتي درعا والقنيطرة. درست بعض الخواس المورفولوجية والمزرعية والبيوكيميائية للمستعمرات وجرى انتخاب العزلات المنتجة لأنزيم السيلولاز وقد تراوح متوسط أقطار الهالات للعزلات المنتجة بين 1و 3.6 سم وكفاءة التحليل بين 1.1 و 4 , تم تصنيف 20 عزلة من اصل 65 عزلة منتجة للأنزيم باستخدام نظام ( API 50 CH) جرى عزل الـ DNA باستخدام طريقة مرجعية معدلة .
In the framework of this research, bacteria belonging to the genus Bacillus were isolated from some Syrian soils in the governorate of Damascus and its countryside, and the governorates of Daraa and Quneitra. Some morphological, agricultural and biochemical traits of the colonies were studied and the isolates producing cellulase enzyme were selected. The average diameters of the halos of the produced isolates ranged between 1 and 3.6 cm and the analysis efficiency ranged between 1.1 and 4, 20 out of 65 isolates producing the enzyme were classified using the (API 50 CH) system. DNA using a modified reference method.
References used
Atlas P 1998 Bile Esculin Test . Exercises for the microbiology laboratory
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The conditions for producing cellulase enzyme were optimized using corn
husks as substrate in submerged fermentation. The effects of five parameters
(incubation temperature, pH, substrate concentration, inoculum volume,
fermentation time) on the s
train growth and enzyme production were studied.
For this purpose the statistical program Minitab and the statistical design
Response Surface Methodology (RSM) were applied.
The study was conducted in 2016. The samples of rice Oryza sativa L. straw
were collected from Dewania governorate/Iraq, and were brought to laboratory of
Directorate of Environmental and Water in Ministry of Science and Technology.
The samples we
re cleaned and milled, then stored in sterile containers. Local
cellulolytic bacterial isolate was cultivated and isolated on mineral and cellulose
medium, at 37 ºC for (24± 2) hours, the bacterial isolate was diagnosed as Bacillus sp.
depending on phenotypes of bacterial colonies on solid medium, microscopic
characters and some biochemical tests. Milled rice straw was chemically treated
with 1% of sodium hydroxide, then biological treatment by bacterial isolate Bacillus sp
cultivate in mineral medium with alkali treated rice straw as carbone source and
compared with cellulose standard medium. The bacterial growth was measured at
600 nm, which reached 0.974 in rice straw medium, while in cellulose medium
reached 0.853. For glucose concentration, the value reached 250 μg/ml in rice straw
medium, while in cellulose medium it was 210 μg/ml. The results concluded the
possibility of getting rid of rice husks, which is an environmental contaminant, and
to use it in the production of glucose.
This research was conducted in the Agriculture Faculty- Food Science
Department, Damascus University, for detecting Lactic acid bacteria isolated
from Syrian dairy products (white fresh cheese-Leben), Samples were collected
from August, 2004 to Oc
tober, 2005 from different areas in Syria. Analysis was
done for 245 bacterial isolates from 40 samples of white fresh cheese and 58
samples of yogurt (curd). Results showed that the genus Enterococcus was
dominant with a percentage of 47.52%; Pediococcus had a percentage of 26.3
%; while Lactococcus had a percentage of 15.38% with only 3.2% for
Lactobacillus.
40 samples were collected from milk from Homs to isolate the
bacteria and study their ability to produce the enzyme β-
galactosidase and diagnose isolates the most efficient in the
production of the enzyme, the results showed the presence of 25
sporeforming isolates producing the enzyme β-galactosidase , the
effectiveness of β-galactosidase was estimated using
spectrophotometer at a wavelength of 420 nm.
Four isolates of Streptomyces sp., St.1 and St.2 were isolated
from garden soil of science faculty, St.3 and St.4 were isolated
from Al-Shameia soil Lattakia, by serial dilution on Starch Casein
Agar medium.
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