The conditions for producing cellulase enzyme were optimized using corn
husks as substrate in submerged fermentation. The effects of five parameters
(incubation temperature, pH, substrate concentration, inoculum volume,
fermentation time) on the s
train growth and enzyme production were studied.
For this purpose the statistical program Minitab and the statistical design
Response Surface Methodology (RSM) were applied.
It was detected for the ability of a local fungus T.harzianum and A.alternate which
was isolated from the rhizosphere of Wheat plant Triticum aestivum L. to produce the
enzyme complex cellulase in solid medium (CMC-Agar). So it has been showed that
T.harzianum and A.alternate have an ability to produce this enzyme, While the
T.harzianum have higher production of cellulase than A.alternate. Then conducted a
quantitative test using Mandelium liquid medium is used to determine the optimal
conditions (incubation time, temperature, PH) for the best cellulase production of the two
fungus studied. It was, the best conditions for its production, from T.harzianum is 7 days
inchubation (3.97 U/mL), temperature 26C (4.07 U/mL) and pH=5.5 (4.19U/mL). While
the best conditions for its production from A.alternate is 8 days inchubation (3.29U/mL),
temperature 28C (3.15 U/mL) and pH=5.0 (3.86 U/mL). The comparison between the
enzymatic activity average for both fungus , was showed superiority T.harzianum by
production cellulase than fungus A.alternate. And discern that the effect of the incubation
time on cellulase production from fungus T.harzianum more than its effect on cellulase
production from fungus A.alternate. While effect of pH and temperature on cellulase
production from fungus T.harzianum less than their effect on cellulase production from
fungus A.alternate.
In this research, protoplast isolations were obtained from the leaves of
Potato Binella cultivar at the laboratories of Agriculture College- Damascus
University in order to determine factors affecting the yield and viability of
protoplast isolation. Results indicated that the best yield percentage of isolated
protoplast.
After morphological and biochemical studies, 11 strains of Actinobacteria
were isolated, mostly of them belong to the genus Streptomyces, these
strains were used later to detect their ability in cellulase and xylanase
enzymes production during the initial detection depending on the diameter
of the halo transparent around cultured colonies on solid media.
