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An HPLC separation of the geometric isomers of β-carotene was developed to evaluate the Provitamin A content of Palm oil . Different stationary phases were studied, the best separation of all-trans-, 9-cis- and 13-cis-β-carotene were obtained with calcium hydroxide columns using isocratic elution . Eluent was nhexane or isooctane or mixtures of them. These techniques were used to determine the provitamin A content of palm oil .
Sweeteners are used as alternatives of Sugar because of their slow absorption and incomplete metabolism in human body, which are positively destinies in setting free small amount of calorie. This research presents the influence of ultrasonic on th e sorbitol synthesis, starting from Glucose by implementing hydrogenation using Lithium Aluminum Hydride. The result we have reached by using the ultrasonic as compared with mechanical mixing or other method have lead us to two conclusions: The first was that we have obtained almost the same quantity of the sorbitol. The second was that by using ultrasonic, micelles of colloid Aluminum hydroxide were accumulated. This accumulation resulted in an easy separation by purification, which eventually lead to a super pure sorbitol solution. We hope that these results will be useful for the Crystallography science.
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