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Register a new userOptimizing the production parameters of baker's yeast (Saccharomyces cerevisiae) using date extract and molasses
أمثلة ظروف إنتاج الكتلة الحيوية لخميرة الخباز باستخدام مستخلص التمر و مولاس التكرير
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Aِl-Baath University ورقة بحثية
Publication date
2014
fields
Agricultural Engineering
and research's language is
العربية
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جمعت 10 عينات من مواد مختلفة من محافظتي دمشق و حمص لعزل و تشخيص الخمائر مورفولوجياً حسب Barnett و فيزيولوجياً باستخدام تقنية API و أظهرت النتائج وجود 6 عزلات من خميرة Saccharomyces cerevisiae , تمت غربلة العزلات المصنفة حسب إنتاجها للكتلة الحيوية, فبينت النتائج تباين السلالات المدروسة في إنتاجها للكتلة الحيوية, و أعطت السلالة المعزولة من التمر أعلى إنتاج للكتلة الحيوية.
Ten randomized samples of materials were collected from two places (Damascus, Homs)in Syria for isolation and identification yeasts. These yeasts were classified morphologically according to Barnett's and physiologically by API technique .Results showed that 6 isolated yeasts were Saccharomyces cerevisiae . These isolated yeasts were screened to investigate production of biomass. The results showed that there were differences in the ability of isolated yeasts to produce biomass. Saccharomyces cerevisiae isolated from date fruit was best yeast for biomass production.
References used
ACOURENE,S.,DJAFRI,K.,AMMOUCH,A.,AMOURACHE,L.,TA MA,M., TALEB B, 2011- Utilization of the date wastes as substrate for the production of baker's yeast and citric acid, Biotechnology, 10: 488-497
ACOURENE,S.,KHALID,K.A.,BACHA,A.,TAMA,M.,TALEB,B.,20 07-Optimization of bakery yeast production cultivated on musts of dates, Journal of applied sciences research, 3: 964-971
AL-MUDHAFFAR, S.,1978- Studies on the conditions of growth of baker's yeast, grown in solution of molasses or debis, Food Nahrung, 22:385-389
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جمعت 30 عينة عشوائية من مواد غذائية مختلفة من محافظتي دمشق وحمص لعزل وتشخيص الخمائر مورفولوجياً حسب Barnett وفيزيولوجياً باستخدام تقنية API
This study was conducted at the laboratories of the Faculty of Agriculture,
Damascus University and the National Commission of Biotechnology in 2012 to
investigate he possibility of Production of bioethanol using local isolate of
Saccharomyces cer
evisiae from raw sugar refining molasses and to determine
the effect of different temperatures, pH and concentration of Dry matter (Brix
degree) in molasses on the amount of ethanol produced. A local isolate (R1) of
Saccharomyces cerevisiae selected previously due to its superiority in ethanol
production was taken from wine brewing residues and exposed to different
temperatures (20, 25, 30 and 35OC), pH (4, 4.5, 5 and 5.5) and the Brix degrees
(16, 20, 24 and 28%) during fermentation.
Results revealed that the best amount of ethanol was produced at
temperature of 30 º C, pH 5, and Brix degree in molasses 16%.
The conditions for producing cellulase enzyme were optimized using corn
husks as substrate in submerged fermentation. The effects of five parameters
(incubation temperature, pH, substrate concentration, inoculum volume,
fermentation time) on the s
train growth and enzyme production were studied.
For this purpose the statistical program Minitab and the statistical design
Response Surface Methodology (RSM) were applied.
The study was conducted in 2016. The samples of rice Oryza sativa L. straw
were collected from Dewania governorate/Iraq, and were brought to laboratory of
Directorate of Environmental and Water in Ministry of Science and Technology.
The samples we
re cleaned and milled, then stored in sterile containers. Local
cellulolytic bacterial isolate was cultivated and isolated on mineral and cellulose
medium, at 37 ºC for (24± 2) hours, the bacterial isolate was diagnosed as Bacillus sp.
depending on phenotypes of bacterial colonies on solid medium, microscopic
characters and some biochemical tests. Milled rice straw was chemically treated
with 1% of sodium hydroxide, then biological treatment by bacterial isolate Bacillus sp
cultivate in mineral medium with alkali treated rice straw as carbone source and
compared with cellulose standard medium. The bacterial growth was measured at
600 nm, which reached 0.974 in rice straw medium, while in cellulose medium
reached 0.853. For glucose concentration, the value reached 250 μg/ml in rice straw
medium, while in cellulose medium it was 210 μg/ml. The results concluded the
possibility of getting rid of rice husks, which is an environmental contaminant, and
to use it in the production of glucose.
Producing of biofuel from crops wastes is considered one of the
advanced eco- friendly technologies. This research was conducted
in the General Commission For Biotechnology in order to study the
possibility to use the juice of sugar sorghum plant
as alternative
substrate to produce bioethanol using S. cerevisiae and optimize
the production’ conditions.
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