In this research two analytical methods were developed for the determination of Azelastin as raw material and in Nasal Spray formulation.
The first method was spectrophotometric while the other used the technic of HPLC The first method based on meas
uring the absorbance at λmax 284 nm .
The relationship between the absorbance and the concentration is rectilinear over the range 0.001 – 0.008 g %.
The proposed spectrophotometric method was successfully applied to the determination of Azelastin in nasal spray.
The percentage recoveries were 99.378±1 (10 duplicate readings)1
No interference was noticed from co-formulated drugs.
For the absorbance at λmax the obtained results were in good agreement with which obtained by the Pharmacopeia method.
The substance gave a good separation while the retention time was 1.467 min.
A simple and rapid analytical method for routine quantification of Hyoscine
butyl bromide (scopolamine butyl bromide) in liquid oral formulations by highperformance
liquid chromatography was developed and validated. The oral
solution samples can be directly analyzed by reversed-phase HPLC on Intersil
ODS-3.
An analytical chromatographic method for determination of total
benzalkonium chloride in eye drops preparations samples containing
gentamicin sulphate, tobramicin and viscous eye drops samples containing
fusidic acid has been developed.
Additive migration levels in food simulants from polymeric materials that
are intended to be into contact with food can be affected by additive stability
under the migration test conditions. In this work, the stability of four
antioxidants: Butyle
ted hydroxy toluene (BHT), Bisphenol A (BPA),
Pentaerythritol tetrakis (3,5-di-tert-butyl-4-hydroxyhydrocinnamate) (Irganox
1010), and Octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate
(Irganox 1076), was studied in aqueous food simulants A, B, C and ethanol 95%
(as a substitute for olive oil- which is a fatty food simulant- simulant D), at
different temperatures 20, 40 and 70 ºC, during 20 days. Samples were
analyzed by high performance liquid chromatography (HPLC) with UV
detector. In general, the studied compounds appeared to be more stable in both
Ethanol (95%) and simulant B than in simulants A and C.
Additive migration levels in food simulants from polymeric materials that
are intended to be into contact with food can be affected by additive stability
under the migration test conditions. In this work, the stability of Dibutyl
adipate (DBA), Di
isobutyl adipate(DIBA), Diisobutyl phthalate (DIBP), Di-noctyl
phthalate (DnOP),and Diethylhexyl sebacate (DEHS).,was studied in
aqueous food simulants A, B, C and ethanol 95% (as a substitute for olive oilwhich
is a fatty food simulant, simulant D), at different temperatures 20, 40 and
70 ºC, during 20 days. Samples were analyzed by high performance liquid
chromatography (HPLC) with UV detector at 270 nm. The stability of the
studied compounds appeared to be least of all in simulant B, then in simulant
A, and finally in simulant C., When temperature and exposure time increase.,
on the other hand, the increase in temperature and exposure time have greatly
less effect on the stability of plasticizers in ethanol %95,as a substitute for olive
oil-which is a fatty food simulant.
Additive migration levels in food simulants from polymeric materials that
are intended to be into contact with food can be affected by additive stability
under the migration test conditions. In this work, the stability of two
plasticizers: Diethyl
hexyl phthalate (DEHP) and Dibutyl phthalate (DBP) was
studies in aqueous food simulants A, B, C and ethanol 95% (as a substitute for
olive oil- which is a fatty food simulant- simulant D), at different temperatures
20, 40 and 70 C0, during 20 days. Samples were analyzed by high performance
liquid chromatography (HPLC) with UV detector.
In general, DEHP and DBP plasticizers appeared to be more stable in both
Ethanol (95%) and simulant C than in simulants A and B, while, they are less
stable, even at low temperatures, in Simulant B than in simulant A .
This paper presents a developed HPLC method for the determination of
caffeine and theobromine in different black and green tea infusion samples,
using different infusion methods, such as infusion with and without boiling,
repeated and continuous i
nfusion. A mixture of double-distilled water and
methanol (60:40 v/v), was used as a mobile phase. In this condition, the
retention time for theobromine was three minutes and for caffeine five minutes.
Ninety-nine randomized samples of milk and white cheeses were collected
from five districts in Damascus city during 2012-2013 to determinef Aflatoxin
M1 using High Performance Liquid Chromatography (HPLC). The procedure
for determination of Aflato
xin M1 was changed according to local
circumnutates which gave sensitive and accurate results during 5.9 minute with
r= 0.99. The average recovery was 83.88% for milk and 102.15% for cheeses
samples, respectively.
In the present work, batch electrocoagulation experiments were carried out
to evaluate the removal of polycyclic aromatic hydrocarbon (PAHs) from water
using aluminum electrodes. The effects of initial pH, current density,
electrolysis time, initi
al concentration of PAHs, electrolyte type, and electrolyte
concentration were investigated to achieve the optimal removal efficiency. The
results indicated that the electrocoagulation utilizing the aluminum, as anode
and cathode, was an efficient tool in the reduction of these contaminants. The
treatment process was found to be largely affected by the current density and
the initial composition of water. The removal rate was significantly increased
using NaCl as an electrolyte where indirect oxidation by hypochlorite forming
later during the treatment was occurred. The results demonstrated that the
technical feasibility of the electrocoagulation as a possible and reliable
technique for the treatment of PAHs contaminants in water.
The purpose of this work is to develop a sensitive, selective and validated
stability–indicating HPLC assay for the analysis of aspartame tablets.
Aspartame and its potential impurities or degradation products are
analyzed on C18 Column using a mo
bile phase containing monobasic
potassium phosphate buffer solution (pH = 3.5) and acetonitrile (85: 15).
