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Register a new userEffect of Rhizobia and Cowpea Severe Mosaic Virus (CPSMV) Interaction on Bacterial Nodulation and Nitrogen Fixation in Cowpea
تأثير التداخل بين التلقيح بالرايزوبيوم و فيروس موزائيك اللوبياء الشديد في تكوين العقد البكتيرية و تثبيت الآزوت في اللوبياء
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Damascus University ورقة بحثية
Publication date
2010
fields
Plant Protecion
and research's language is
العربية
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Shamra Editor
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أجريت هذه الدراسة لتقويم كفاءة تأثير معاملة البذور بـالرايزوبيوم و عـدوى النباتـات بفيـروس موزائيك اللوبياء الشديد CPSMV في تكوين العقد و تثبيـت الآزوت فـي اللوبيـاء. عـزل الفيـروس CPSMV من نباتات مصابة في كلية الزراعة جامعة بغداد – أبو غريب - العراق، و عزلت الرايزوبيوم من عقد على جذور نباتات اللوبياء. أعديت النباتات المعاملة بالفيروس بعد أسبوعين من الإنبات. تكونت العقد على جذور النباتات المعاملة بالرايزوبيوم في حين لم يلاحظ تكون العقد على جـذور نباتـات غيـر معاملة بالرايزوبيوم. وجد أن معدل عدد العقد كان 93.24 عقدة/نبات في النباتـات المعـداة بـالفيروس مقارنة بـ 86.59 عقدة/نبات في النباتات غير المعداة. سبب الفيروس انخفاضاً معنوياً في وزن العقـد، فقد بلغ 62.0 غم/عقدة في النباتات المعداة مقارنة بـ 13.2 غم/عقدة في النباتات غير المعداة. وجد أن الرايزوبيوم أحدثت زيادة معنوية في نسبة الآزوت في المجموع الخضري عند المستوى (05.0=p) فقـد بلغت 502.2 % في النباتات المعاملة بالرايزوبيوم و المعداة بالفيروس 550.2 % في النباتـات المعاملـة بالرايزوبيوم و غير المعداة بالفيروس مقارنة بـ 29.1 % في النباتات غير المعاملة بـالرايزوبيوم و غيـر المعداة بالفيروس. و سبب الفيروس زيادة معنوية في نـسبة الآزوت 788.1 % فـي النباتـات المعـداة بالفيروس مقارنة بـ 29.1 % في النباتات غير المعداة. أدت الرايزوبيوم إلى زيادة في عدد القرون و عدد البذور في النبات و وزن البذور الجاف في النبات فكانت 6.26 و 114 و 4.32 مقارنـة بــ 8.12 و 6.70 و 7.8 بالتتابع. أدت معاملة النباتات المعداة بالفيروس بالرايزوبيوم إلى زيادة معنويـة فـي هـذه القـيم باستثناء نسبة الآزوت في البذور إذ بلغت 4.16 و 6.34 و 4.10 بالتتابع.
This study was conducted to evaluate the effect of seed Rhizobia-treating and Cowpea severe mosaic virus (CPSMV) inoculation on bacterial nodulation and nitrogen fixation in cowpea. CPSMV was isolated from infected plants, College of Agriculture, Abu-Ghraib, Baghdad, IRAQ, the Rhizobia was isolated from nodules on roots of cowpea plants. The plants were inoculated by the virus 2 weeks after germinations. Nodules on roots of Rhizobia-treated plants were formed while no nodules were observed on non-treated ones. The mean number of nodules was 24.93\ plant on virus inoculated plants compared to 59.86 nodules\plant on non-inoculated ones. Significant reduction in nodules weight was induced by the virus, 0.62 gm on the virus inoculated plants compared to 2.13gm on non-inoculated ones. It has been found that the Rhizobia induced significant increase in nitrogen percentage at p=0.05 in the foliage, 1.29% in plants non-treated with Rhizobia non-inoculated by the virus compared with 2.502% in Rhizobia treated-virus inoculated plants and 2.550% in Rhizobia treated non inoculated plants. The virus caused an increase in nitrogen percentage, 1.877% compared to 1.29% in non-inoculated plants. The Rhizobia caused an increase in pods number\plant, seed number\plant, and seed dry weight (gm)\plant, 25.6, 114, 32.4 compared to 12.8, 70.6, 8.7 in non-treated plants respectively. The treatment of virus inoculated plant by Rhizobia induced a significant increase in these values, except with nitrogen percentage in seeds, 16.4, 84.6, 10.4, 4.3 respectively.
References used
Arras, G. and S. Arru. (1997). Mechanisms of action of some microbial antagonists against fungal pathogens. Ann. Microbiol. Enzymol. 47 : 97 – 120
Burton, J. C., C. J. Martinez and R. L. Curley. (1972). Methods of testing and suggested standard for legume inoculation and pre-inoculated seed. Copyright by Nitrogen sales crop
Chen, L. S., A. Figueredo, and H. Villani. (2002). Diversity and symbiotic effectiveness of rhizobia isolated from field-grown soybean nodules in Paraguay. Biol. Fertl. Soils 35 : 448 – 457
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