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Register a new userSurvey of natural transmission of some viruses by cowpea seeds
التحري عن الانتقال الطبيعي لبعض الفيروسات ببذور اللوبياء
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Tishreen University ورقة بحثية
Publication date
2013
fields
Plant Protecion
and research's language is
العربية
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Shamra Editor
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أشارت نتائج اختيار البصمة النسيجية المناعية (TBIA) لـ 754 بذرة لوبياء جمعت من مناطق مختلفة من محافظة اللاذقية انتقال فيروسٍ واحدٍ فقط و هو فيروس موزاييك البازلياء المنقول بالبذور (PSbMV) من بين خمسة فيروسات استخدمت أمصالها في الاختيار (CMV, BYMV, PSbMV, BBMV and AMV). و تضمنت جميع المصادر بذوراً تحمل الفيروس و كانت نسبة الانتقال عالية حيث تراوحت ما بين 24,64% و 37,50 %. تُعدّ هذه النتيجة التسجيل الأول محلياً لانتقال فيروس موزاييك البازلياء المنقول بالبذور إلى بذور اللوبياء.
The results of Tissue-blot immunobinding assay (TBIA) of 754 samples of cowpea seeds collected from various regions in Lattakia province has showed the transmission of Pea seed borne mosaic virus (PSbMV) among five virus studied (CMV, BYMV, PSbMV, BBMV and AMV). Pea seed borne mosaic virus was detected on seeds collected from various locations in 24, 64% to 37, and 50%. This result is the first record of seed transmission of PSbMV in cowpea seeds.
References used
Al-BRECHTESN, S. E.; Seed-borne viruses (lecture notes) Danish Government Institute of seed pathology for Developing countries,1997,34 pp
BASHIR, M, and HAMPTON, R.O.: Detection and identification of seed-borne viruses from cowpea (vigna unguculata (L) walp) germplasm. plant pathology. 1996, 45:54- 58
BASHIR, M, & HAMPTON, R, O.; Serological and biological comparisons of blackeye cowpea mosaic and cowpea aphid-borne mosaic potyvirus isolates seed-borne in vigna unguiculate (L) walp germplasm. Journal of phytopathologyphtopathologhische Zeitschrift. 1996, 144, 257-263
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This study was conducted to evaluate the effect of seed Rhizobia-treating
and Cowpea severe mosaic virus (CPSMV) inoculation on bacterial nodulation
and nitrogen fixation in cowpea. CPSMV was isolated from infected plants,
College of Agriculture,
Abu-Ghraib, Baghdad, IRAQ, the Rhizobia was isolated
from nodules on roots of cowpea plants. The plants were inoculated by the
virus 2 weeks after germinations. Nodules on roots of Rhizobia-treated plants
were formed while no nodules were observed on non-treated ones. The mean
number of nodules was 24.93\ plant on virus inoculated plants compared to
59.86 nodules\plant on non-inoculated ones. Significant reduction in nodules
weight was induced by the virus, 0.62 gm on the virus inoculated plants
compared to 2.13gm on non-inoculated ones. It has been found that the
Rhizobia induced significant increase in nitrogen percentage at p=0.05 in the
foliage, 1.29% in plants non-treated with Rhizobia non-inoculated by the virus
compared with 2.502% in Rhizobia treated-virus inoculated plants and 2.550%
in Rhizobia treated non inoculated plants. The virus caused an increase in
nitrogen percentage, 1.877% compared to 1.29% in non-inoculated plants. The
Rhizobia caused an increase in pods number\plant, seed number\plant, and seed
dry weight (gm)\plant, 25.6, 114, 32.4 compared to 12.8, 70.6, 8.7 in non-treated
plants respectively. The treatment of virus inoculated plant by Rhizobia
induced a significant increase in these values, except with nitrogen percentage
in seeds, 16.4, 84.6, 10.4, 4.3 respectively.
The experiment was conducted at the vegetable research farm, Depart.of
Hort. and Forst. Resea., Nenivah during the growing season of ١٩٩٤ and ١٩٩٥.
The objective of the research was to investigate the effect of plant spacing
(١٠, ٢٠, ٣٠ cm) and phosphorus fertilizer (٠, ٢٥, ٥٠, ٧٥ kg.P٢O٥/donum),on
yield and other related traits of Cowpeas.
Afield survey of ckickpea chlorotic stunt virus (CPCSV) was conducted in some plantation areas in Lattakia province. A total of 252 samples were collected from plants showing chlorotic and stunting symptoms from 54 field. Results of Tissue Blot Immun
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Formaldehyde is classified by the World Health Organization (International Agency
for Research on Cancer) as a carcinogen in Group 1. The upper limit allowed for use in
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detect and
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using acetyl acetone method. Formaldehyde was detected in 77% of the studied cosmetic
products (49 samples) despite the fact that most of them were not labeled formaldehyde or
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samples were higher than the allowed limit (0.34% -11.73%) except one sample 0.18%. pH
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A field survey of Pepper mild mottle (PMMV) and Cucumber mosaic (CMV)
Viruses was conducted in some plantation areas in Tartous and Lattakia Provinces. A total
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Tis
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Provinces. In this study, PMMV was not recorded in Lattakia, whereas it was recorded in
Tartous in a small percentage (0.64%).
Using Tissue Blot Immunobinding Assay, the collected seeds from the infected
pepper fruits from Tartous fields showed the transmission of PPMV throughseeds in a ratio
of 0.70% to 0.87% according to seed resources, whereas CMV was transmitted in a high
percentage of 2.81% to 7.98% according to seed resources. PPMV was not recorded in leaf
samples or in seeds taken from Blozah District in TartousProvince.
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