Do you want to publish a course? Click here

Identification of Pseudomonas savastanoi pv.savastanoi isolated from myrtle plant (Myrtus communis ) in Syria

تعريف بكتريا Pseudomonas savastanoi pv.savastanoi المعزولة من نبات الآس Myrtus communis في سورية

2082   1   53   0 ( 0 )
 Publication date 2003
and research's language is العربية
 Created by Shamra Editor




Ask ChatGPT about the research

Olive trees are grown in many regions of Syria, especially in wet regions where the olive knot disease (Pseudomonas savastanoi pv.savastanoi ) prevails .Symptoms similar to those found on olive trees have been observed on myrtle shrubs (Myrtus communis) growing naturally in some olive-grown regions. This study aimed at identifying the pathogen isolated from myrtle plant, and testing the pathogenicity of these isolates on olive trees. Morphological, biochemical and serological tests of bacteria isolated from myrtle showed similarity to those from olive and other hosts. Pathogenicity tests showed that the myrtle isolates were pathogenic on both myrtle and olive trees. Myrtle could be considered as a source of inoculum for the olive knot disease.


Artificial intelligence review:
Research summary
تتناول هذه الدراسة تحديد بكتيريا Pseudomonas savastanoi pv. savastanoi المعزولة من نبات الآس (Myrtus communis) في سوريا. تُعرف هذه البكتيريا بأنها المسبب الرئيسي لمرض عقد الزيتون الذي ينتشر في مناطق زراعة الزيتون الرطبة في سوريا. لاحظ الباحثون أعراضًا مشابهة لتلك الموجودة على أشجار الزيتون على شجيرات الآس التي تنمو بشكل طبيعي في بعض المناطق المزروعة بالزيتون. هدفت الدراسة إلى تحديد العامل الممرض المعزول من نبات الآس واختبار قدرته على إحداث المرض على أشجار الزيتون. أظهرت الاختبارات المورفولوجية والكيميائية الحيوية والمصلية للبكتيريا المعزولة من الآس تشابهًا مع تلك المعزولة من الزيتون والمضيفات الأخرى. أظهرت اختبارات الإمراضية أن عزلات الآس كانت ممرضة لكل من الآس وأشجار الزيتون، مما يشير إلى أن الآس يمكن اعتباره مصدرًا للعدوى بمرض عقد الزيتون.
Critical review
دراسة نقدية: تعتبر هذه الدراسة مهمة لأنها تسلط الضوء على مصدر جديد محتمل لمرض عقد الزيتون، وهو نبات الآس. ومع ذلك، يمكن توجيه بعض النقد للدراسة. أولاً، كان من الأفضل توسيع نطاق الدراسة لتشمل مناطق جغرافية أخرى لضمان تعميم النتائج. ثانيًا، كان يمكن تقديم تفاصيل أكثر حول الإجراءات التجريبية لضمان إمكانية تكرار الدراسة من قبل باحثين آخرين. وأخيرًا، كان من الممكن تقديم تحليل أعمق للبيانات المصلية والبيوكيميائية لتعزيز قوة النتائج.
Questions related to the research
  1. ما هو الهدف الرئيسي من الدراسة؟

    الهدف الرئيسي من الدراسة هو تحديد بكتيريا Pseudomonas savastanoi pv. savastanoi المعزولة من نبات الآس واختبار قدرتها على إحداث المرض على أشجار الزيتون.

  2. ما هي الأعراض التي لوحظت على شجيرات الآس؟

    لوحظت أعراض مشابهة لتلك الموجودة على أشجار الزيتون المصابة بمرض عقد الزيتون على شجيرات الآس.

  3. ما هي الاختبارات التي أجريت لتحديد البكتيريا؟

    أجريت اختبارات مورفولوجية وكيميائية حيوية ومصلية لتحديد البكتيريا المعزولة من الآس.

  4. ما هي النتيجة الرئيسية التي توصلت إليها الدراسة؟

    النتيجة الرئيسية هي أن عزلات الآس كانت ممرضة لكل من الآس وأشجار الزيتون، مما يشير إلى أن الآس يمكن اعتباره مصدرًا للعدوى بمرض عقد الزيتون.


References used
BRADBURY J. F., 1986. Guide to plant pathogenic bacteria. CAB International,Mycological Institute, 332P
BROWN N, A., 1932. Canker of ash trees produced by a variety of the olive – tubercle organism. Bacterium savastanoi. Jornal of Agricultural Research . Washington, D. C., 44, 702 – 722
GARDAN, L.; BOLLET, C.; ABU – GHORRAH, M.; GRIMONT, F. and GRIMONT P.A.D., 1992. DNA relatedness among the pathovar strains of Pseudomonas syringae subsp. savastanoi. Janse (1982) and proposal of Pseudomonas savastanoi sp.nov. Int. J Syst. Bacteriol. , 42 , 606 – 612
rate research

Read More

This research was carried out to determine the quantity of essential oil extracted from myrtle Myrtus communis L leaves, by hydro distillation, and to identify the major and minor components, besides to determine its quantities in the essential oil by Gas Chromatography-Mass Spectrometry GC-MS. The samples were collected on November 2013 from eight different climatic locations (Aleppo, Zarzour, Alzenea, Katra AL-Rayhan, Al- Rayhana, Kasab, Mashta al-Holou and Mosiaf). The quantity and composition of the essential oil were estimated on the basis of the leaves dry weight. Oil quantity varied between the studied genotypes from different locations, which range from 0.46 to 0.55 ml, with non-significant differences. Eighteen compounds were identified in myrtle essential oil, and there were significant differences in 13 compounds. 1,8-Cineole performed the highest rate (18.85%) compared with other components, followed by α-Pinene (16.93%). While the lowest percentage was (0.31%) of Myrcene. The
Single nodes and axillary buds excised from adult trees of Myrtle (Myrtus communis L.) grown in the field under natural conditions at Damascus countryside (Ain Elfiegh) were used as primary explants, which were surfacedisinfected by 70% Ethanol an d Clorox containing 5.25 % Sodium Hypoclorite with a drop of Tween 20 for different periods and concentrations before being placed onto MS basal medium. Established cultures were then transferred onto MS basal medium containing a combination of growth regulators at different concentrations (BA at 2.22 and 4.44 μM) each with NAA at 0.54, 1.62, 5.4 μM or 1.47 μM IBA with GA3 at 0.58 μM for all tratments. Multiplication rate of 12.8-fold was achieved every 4 weeks on MS medium supplemented with 4.44 μM BA with 1.47 μM IBA and GA3 at 0.58 μM.
Bioeffect of some Syrian Myrtus communis L. leaves extracts in growth of some pathogenic microorganisms, which were isolated from Al-Assad hospital laboratory in Lattakia, was tested by disc diffusion method. Results showed that cold and hot water ex tracts have antibacterial activity against all Gram positive and negative bacteria, and pathogenic Candida albicans except Klebsiella pneumoniae. All organic extracts have antibacterial activity against Proteus vulgaris, Entrococcus faecium, Staphylococcus aureus and Staphylococcus albus. Pseudomonas aeruginosa and Pseudomonas fluorescens were affected by most of the organic extracts, and the most bioactivity was by methanol extract, and all inhibition zones of extracts were bigger than inhibition zones by control antibiotics. Serratia marcescens and Acinetobacter calcoaceticus were affected by methanol (with highest inhibition zone 37.33 and 27.33 mm respectively), ethanol, acetone and ethyl acetate extracts, but resistant to the others. Klebsiella pneumoniae affected by ethanol extract only with inhibition zone 20.33 mm. Results of bioactivity according to a various solvents used in this study demonstrate that Syrian Myrtus communis L. leaves extracts have antimicrobial activity, so expected to be potential sources of natural antibacterial and antifungal products against some pathogenic bacteria and fungi in the future.
Isolation and identification of Pseudomonas aeruginosa was screened from 15 samples of Syrian oily-soils to determine the ability to produce Lipase enzyme by Cetermide Agar, Tributrin Agar and Brain Heart Broth during 2007-2008 AD. The results sho wed that there were grown colonies with color of green or yellow-green surrounded with different diameter of clear zone in three samples and the identification by Mowasat Hospital revealed that it was Pseudomonas aeruginosa In addition, the results showed that the optimum conditions to produce lipase was 35 Cº with the pH degree of 8 and the time of production of lipase with highest activity of 63.3% was three days in submerged culture during the optimum conditions. These results illustrated the important of this isolates to produce and control lipase for industrial production.
Symptoms of soft rot showed in some fields cultivated with garlic crop in the Kissweh region (Damascus countryside). Studying this disease, found that the incidence rates ranging between 2.91% to 5.59%. 55 bacterial isolates were isolated from inf ected samples collected during the seasons 2005 and 2006. The results of five biochemical tests showed that 22 isolates were pectinolitic and they were pathogenic to garlic plant and gave symptoms similar to those observed in the field, theses isolates were classified as belonging to the genus Pectobacterium. To determine the species of the bacteria, they have been characterized towards nine biochemical tests, which allowed the classification of 7 isolates as Pectobacterium carotovorum subsp. carotovorum, 6 isolates belonged to Pectobacterium atrosepticum, one isolate to Dickeya chrysanthemi (Pectobacterium chrysanthemi), 6 isolates have not been classified and 2 isolate did not characterized . These results have shown for the first time the isolation and identification of the Pectobacterium bacteria causing soft rot on garlic crop in Syria.
comments
Fetching comments Fetching comments
Sign in to be able to follow your search criteria
mircosoft-partner

هل ترغب بارسال اشعارات عن اخر التحديثات في شمرا-اكاديميا