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Register a new userMono and bi-cationic influence on the enzymatic activity of many oxidoreductaze enzymes
تأثير عدد من الشوارد الأحادية و ثنائية التكافؤ في الفعالية الإنزيمية لعدد من إنزيمات الأكسدة و الإرجاع
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Damascus University ورقة بحثية
Publication date
2014
fields
Chemistry
and research's language is
العربية
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Shamra Editor
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هدف البحث المقدم إلى دراسة التغيرات التي تطرأ على فعالية عدد من إنزيمات الأكسدة و الإرجاع في خلايا بادرات نبات الذرة الصفراء عند تنميتها بمحاليل الشوارد الأحادية و الثنائية الآتية +Na+, K+, Mg2+, Ca2. استخدمت في العمل بذور الذرة الصفراء من النوع غوطة 82 بعد تنميتها مدة 6 أيام في الوسط المائي و في أوساط تحوي على تراكيز مختلفة من محاليل الشوارد المدروسة. تتلخص مراحل المعالجة و المعايرة باستخلاص المستحضرات الإنزيمية المدروسة من خلايا ساق البادرات بواسطة المحاليل الموقية (في قيمpH الملائمة لكل إنزيم). بينت النتائج التي تم الوصول إليها أن فعالية الإنزيمات المدروسة تتزايد بارتفاع تراكيز الشوارد المدروسة من 1 إلى 6 غ/ل مقارنة بفعاليتها في العينات المنماة في الوسط المائي. في حين أظهر استخدام تراكيز من العناصر الأحادية بين 6 و 9غ/ ل اختلافاً في الفعالية.
This research aims to study the effects of some oxidoreductase enzymes activity in the seedlings of maize plant stem cells, which Cultured in the present of the following cations: Na+, K+, Mg2+, and Ca2+. (GHOTA 82) yallow corn seeds were used, and cultured for six days in water and in solution of cations with different concentrations. In brief, preparation and titration processes consist of extraction of enzymatic solutions from plant stem cells seedlings by buffer solutions (at appropriate PH for each enzyme). The results show that the activity of used enzymes increases with the increasing concentrations of the used cations from 1 to 6 g/l by comparison with those of cultured samples in water. Besides the difference in the effect of cations was limited by the concentrations range between 6-9 g/ l.
References used
Silva, J. A., Uchida, R. 2000. Essential Nutrients for Plant Growth: Nutrient Functions and Deficiency Symptoms, College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa
Plant Nutrition. 2011. Innovate to Conserve Natural Resources MGP Inc, 1(800), 574-7248 23/3/2013
Mineral Nutrition of Plants: Principles and Perspectives. 2009. Emanuel Epstein – 12/1/2012
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