الهدف من هذا البحث تحديد تأثير الجسم الأصفر في مراحله المختلفة على الخصائص الشكلية للمعقّد الخلوي )خلايا ركامية-بييضة)، و بالتالي على مردودية البييضات الصالحة شكلياً للتطوّر المختبري In vitro .
جمعت البييضات من مبايض الأبقار بعد الذبح ، و قسّمت المبيايض إلى أربع مجموعات :
آ- مبايض مع جسم نزفي .
ب- مبايض مع جسم أصفر نشط وظيفياً .
ج- مبايض مع جسم أصفر متراجع .
د – مبايض بدون جسم أصفر .
بينت النتائج في هذا البحث أنّ مردودية البييضات الصالح للتطوّر المختبري اختلفت وفقا لمرحلة الطور الأصفري . و كانت أعلى قيمة للمردودية في المبايض التي لاتحتوي جسما ًأصفراً ( 1.6±8.1 ) ، بينما أقلها في مرحلة الجسم الأصفر المتراجع، إذ بلغت (1.3±5.5). و قد كان الفرق معنوياً p < 0.001)).
The purpose of this research was to determine the effect of the corpus luteum in
various stages on morphological characteristics of cumulus oocyte complex consequently
on the yield of morphologically intact oocytes for in vitro culturing.
Oocytes were collected from cow ovariesof animals after slaughter.
Ovaries were divided into the following four groups: (a) ovaries with corpus
hemorrhagicum; (b) ovaries with a functional corpus luteum;
(c) ovaries with regressing corpus luteum (albicans) ;( d) ovaries without any luteal
structure.
The results of this research show that, the intact oocytes yield for in vitro culturing
differs accordingly with the stage of the corpus luteum.
The highest percentage of intact cell(8.1 ± 1.6) were recorded in ovaries without
corpus luteum , whereas the lowest percentage of intact cells were recorded in ovaries with
regressing corpus luteum(5.5 ± 1.3). There was significant difference (p < 0.001)between
the two groups for intact cells.
References used
ABDOON,A.S.S. Factors affecting follicular population ,oocyte yield and quality in camels(Camelus dromedaries ) ovary with special reference to maturation time in vitro. Animal Reproduction Science 66,2001,71-79
ADAMS,G.P.; JAISWAL,R. ; SINGH,J. ;MALHI,P. Progress in understanding ovarian follicular dynamics in cattle. Theriogenology,69,2008,72-80
MAHMOUD E.A.; ABOUEL-ROOS and ALAA E. ABDEL-GHAFFAR. Effect of Season of the year and Ovarian Structures on Oocytes Recovery Rate, Quality and Meiotic Competence in Egyptian Buffaloes .Global Veterinaria 10 (4), , 2013, 408- 412
The aim of this research is to determine the effect of seasonal changes on the
yield of morphologically intact oocytes for in vitro maturation and consequently
for fertilization and embryo production. Determining the impact of seasonal
changes hel
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This current study was conducted on gardenia plant (Gardenia
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