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Effect of The Corpus Luteum on the Yield of Local Cow Intact Oocytes for in vitro Culturing

تأثير الجسم الأصفر على مردودية البييضات الصالحة للتطور In vitro عند الأبقار المحلية

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 Publication date 2014
and research's language is العربية
 Created by Shamra Editor




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The purpose of this research was to determine the effect of the corpus luteum in various stages on morphological characteristics of cumulus oocyte complex consequently on the yield of morphologically intact oocytes for in vitro culturing. Oocytes were collected from cow ovariesof animals after slaughter. Ovaries were divided into the following four groups: (a) ovaries with corpus hemorrhagicum; (b) ovaries with a functional corpus luteum; (c) ovaries with regressing corpus luteum (albicans) ;( d) ovaries without any luteal structure. The results of this research show that, the intact oocytes yield for in vitro culturing differs accordingly with the stage of the corpus luteum. The highest percentage of intact cell(8.1 ± 1.6) were recorded in ovaries without corpus luteum , whereas the lowest percentage of intact cells were recorded in ovaries with regressing corpus luteum(5.5 ± 1.3). There was significant difference (p < 0.001)between the two groups for intact cells.


Artificial intelligence review:
Research summary
تهدف هذه الدراسة إلى تحديد تأثير الجسم الأصغر في مراحله المختلفة على الخصائص الشكلية لمجمع الخلايا الركامية-البييضة، وبالتالي على مردودية البييضات الصالحة للتطور المختبري عند الأبقار المحلية. تم جمع البييضات من مبايض الأبقار بعد الذبح وتقسيم المبايض إلى أربع مجموعات: مبايض تحتوي على جسم نزفي، مبايض تحتوي على جسم أصغر نشط، مبايض تحتوي على جسم أصغر متراجع، ومبايض بدون جسم أصغر. أظهرت النتائج أن أعلى مردودية للبييضات السليمة كانت في المبايض التي لا تحتوي على جسم أصغر، بينما كانت أقل مردودية في المبايض التي تحتوي على جسم أصغر متراجع. تم تحليل البيانات باستخدام تحليل التباين ANOVA، وأظهرت النتائج فروقات معنوية بين المجموعات المختلفة. توصي الدراسة بضرورة إيجاد معايير أخرى مكملة لتقليل التباين في تقييم البييضات، واعتماد المبايض التي لا تحتوي على جسم أصغر كمصدر للحصول على أعلى مردودية من البييضات الصالحة للتطور المختبري.
Critical review
دراسة نقدية: تقدم هذه الدراسة معلومات قيمة حول تأثير الجسم الأصغر على مردودية البييضات الصالحة للتطور المختبري عند الأبقار المحلية. ومع ذلك، هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الممكن أن تكون العينة أكبر للحصول على نتائج أكثر دقة وشمولية. ثانياً، يمكن أن تكون هناك عوامل أخرى غير مدروسة تؤثر على مردودية البييضات، مثل الظروف البيئية والعوامل الوراثية، والتي لم يتم تناولها بشكل كافٍ في هذه الدراسة. أخيراً، توصي الدراسة بإجراء دراسات مقارنة مع أنواع أخرى من الحيوانات، وهو ما يمكن أن يكون خطوة مهمة لتحسين فهمنا لتأثير الجسم الأصغر على مردودية البييضات في مختلف الأنواع.
Questions related to the research
  1. ما هو الهدف الرئيسي من هذه الدراسة؟

    الهدف الرئيسي من الدراسة هو تحديد تأثير الجسم الأصغر في مراحله المختلفة على الخصائص الشكلية لمجمع الخلايا الركامية-البييضة، وبالتالي على مردودية البييضات الصالحة للتطور المختبري عند الأبقار المحلية.

  2. ما هي المجموعات الأربع التي تم تقسيم المبايض إليها في الدراسة؟

    تم تقسيم المبايض إلى أربع مجموعات: مبايض تحتوي على جسم نزفي، مبايض تحتوي على جسم أصغر نشط، مبايض تحتوي على جسم أصغر متراجع، ومبايض بدون جسم أصغر.

  3. ما هي النتائج الرئيسية التي توصلت إليها الدراسة؟

    أظهرت النتائج أن أعلى مردودية للبييضات السليمة كانت في المبايض التي لا تحتوي على جسم أصغر، بينما كانت أقل مردودية في المبايض التي تحتوي على جسم أصغر متراجع.

  4. ما هي التوصيات التي قدمتها الدراسة لتحسين مردودية البييضات الصالحة للتطور المختبري؟

    توصي الدراسة بضرورة إيجاد معايير أخرى مكملة لتقليل التباين في تقييم البييضات، واعتماد المبايض التي لا تحتوي على جسم أصغر كمصدر للحصول على أعلى مردودية من البييضات الصالحة للتطور المختبري.


References used
ABDOON,A.S.S. Factors affecting follicular population ,oocyte yield and quality in camels(Camelus dromedaries ) ovary with special reference to maturation time in vitro. Animal Reproduction Science 66,2001,71-79
ADAMS,G.P.; JAISWAL,R. ; SINGH,J. ;MALHI,P. Progress in understanding ovarian follicular dynamics in cattle. Theriogenology,69,2008,72-80
MAHMOUD E.A.; ABOUEL-ROOS and ALAA E. ABDEL-GHAFFAR. Effect of Season of the year and Ovarian Structures on Oocytes Recovery Rate, Quality and Meiotic Competence in Egyptian Buffaloes .Global Veterinaria 10 (4), , 2013, 408- 412
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