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Effect of Seasonal Changes on the Yield of Local Cow Intact Oocytes for in vitro Culturing

تأثير التبدلات الفصلية في مردودية البييضات الصالحه للتطور In Vitro عند الأبقار المحلية

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 Publication date 2003
and research's language is العربية
 Created by Shamra Editor




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The aim of this research is to determine the effect of seasonal changes on the yield of morphologically intact oocytes for in vitro maturation and consequently for fertilization and embryo production. Determining the impact of seasonal changes helps us to increase the intact oocytes yield, and better use of in vitro culturing technique of cow oocytes. This leads to: benefit from animals of genetic superiority, maintenance the biodiversity of animal genetic resources by using deep-freezing technique of pre/post in vitro fertilized oocytes taken from cow ovary of animals after slaughter. The results of this research paper show that, the intact oocytes yield for in vitro culturing differs accordingly with year seasons. The lowest percentage of intact cells 29.5 were recorded in the period between May and September, whereas the highest percentage of intact cells 42.6% were recorded in the period between October and March. There was significant difference (P<0.001) between the two periods for intact cells.


Artificial intelligence review:
Research summary
تهدف هذه الدراسة إلى تحديد تأثير التغيرات الموسمية على إنتاج البويضات السليمة شكليًا لنضجها في المختبر وبالتالي للتخصيب وإنتاج الأجنة. يساعد تحديد تأثير التغيرات الموسمية على زيادة إنتاج البويضات السليمة، والاستخدام الأفضل لتقنية الزراعة في المختبر لبويضات الأبقار. تظهر نتائج هذه الدراسة أن إنتاج البويضات السليمة للزراعة في المختبر يختلف وفقًا لفصول السنة. تم تسجيل أدنى نسبة للخلايا السليمة 29.5% في الفترة بين مايو وسبتمبر، في حين تم تسجيل أعلى نسبة للخلايا السليمة 42.6% في الفترة بين أكتوبر ومارس. كان هناك فرق كبير (P<0.001) بين الفترتين للخلايا السليمة.
Critical review
دراسة نقدية: تعتبر هذه الدراسة مهمة في مجال تربية الحيوانات وإنتاج الأجنة، حيث تسلط الضوء على تأثير التغيرات الموسمية على جودة البويضات. ومع ذلك، يمكن توجيه بعض النقد البناء للدراسة. أولاً، قد يكون من المفيد توسيع نطاق الدراسة لتشمل أنواع أخرى من الحيوانات لتعميم النتائج. ثانيًا، يمكن تحسين الدراسة من خلال تضمين عوامل بيئية أخرى مثل الرطوبة والتغذية التي قد تؤثر على جودة البويضات. أخيرًا، يمكن تعزيز الدراسة من خلال تقديم توصيات عملية للمزارعين لتحسين إنتاجية البويضات في الفصول المختلفة.
Questions related to the research
  1. ما هو الهدف الرئيسي من هذه الدراسة؟

    الهدف الرئيسي هو تحديد تأثير التغيرات الموسمية على إنتاج البويضات السليمة شكليًا لنضجها في المختبر وبالتالي للتخصيب وإنتاج الأجنة.

  2. ما هي أدنى نسبة للخلايا السليمة التي تم تسجيلها في الدراسة؟

    أدنى نسبة للخلايا السليمة التي تم تسجيلها هي 29.5% في الفترة بين مايو وسبتمبر.

  3. ما هي الفروق الإحصائية بين الفترتين المختلفتين من السنة فيما يتعلق بإنتاج البويضات السليمة؟

    كان هناك فرق كبير (P<0.001) بين الفترتين المختلفتين من السنة فيما يتعلق بإنتاج البويضات السليمة.

  4. كيف يمكن أن تساهم نتائج هذه الدراسة في تحسين استخدام تقنية الزراعة في المختبر لبويضات الأبقار؟

    يمكن أن تساهم نتائج هذه الدراسة في تحسين استخدام تقنية الزراعة في المختبر لبويضات الأبقار من خلال زيادة إنتاج البويضات السليمة والاستفادة من الحيوانات ذات التفوق الجيني والحفاظ على التنوع البيولوجي للموارد الجينية الحيوانية.


References used
Al-Katanani, Y. M., Paula-Lopes F. F. and Hansen P. J. (2002). Effect of season and exposure to heat stress on Oocyte competence in Holstein cows. J. Dairy Sci. 85: 390-396
Asdel, S. A. (1964). Patterns of mammalian reproduction. London: Constable & Co. Ltd, 586 – 588
Badinga, L., Thatcher W. W., Diaz T., Drost M. and Wolfenson, D. (1993). Effect of environmental heat stress on follicular development and steroidogenesis in Lactating Holstein cows. Theriogenology 39:797-810
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