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Register a new userProduction of bioethanol using local isolate of Saccharomyces cerevisiae from raw sugar refining molasses
إنتاج الإيثانول الحيوي من المولاس الناتج عن تكرير السكر الخامي باستخدام عزلة محلية من خميرة Saccharomyces cerevisiae
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اختبرت في هذا البحث إمكانية استثمار مولاس تكرير السكر الخام فـي إنتـاج كحـول الإيتـانول باستخدام العزلة المتفوقة R1 التي اختيرت في بحث سابق من 20 عزلة محلية لخميـرة cerevisiae Saccharomyces لتفوقها في إنتاج الكحول من مولاس الشوندر السكري المحلي. أبـدت العزلـة R1 المأخوذة من راسب تخمير النبيذ من مصنع للمشروبات في منطقة كفرام أيضاً تفوقها في انتاج الايتانول من ركيزة المولاس الناتج من تكرير السكر الخام على باقي العزلات، و درس بعد ذلـك تـأثير درجـات الحرارة 20 و 25 °و 30 °و 35°م و درجات الحموضة (pH (4 و 5. 4 و 5 و 5.5 و تركيز المادة الجافـة (Bx (16 % و 20 % و 24 % و 28 % في قدرة هذه العزلة في إنتاج الإيثانول من المولاس النـاتج عـن تكرير السكر الخام. أثبتت الدراسة أن أفضل مردود من الإيثانول باستخدام العزلة R1 قد تَحقّق عند درجة حرارة 30 م و pH 5 و تركيز مادة جافة (Bx) في المولاس 16%.
This study was conducted at the laboratories of the Faculty of Agriculture, Damascus University and the National Commission of Biotechnology in 2012 to investigate he possibility of Production of bioethanol using local isolate of Saccharomyces cerevisiae from raw sugar refining molasses and to determine the effect of different temperatures, pH and concentration of Dry matter (Brix degree) in molasses on the amount of ethanol produced. A local isolate (R1) of Saccharomyces cerevisiae selected previously due to its superiority in ethanol production was taken from wine brewing residues and exposed to different temperatures (20, 25, 30 and 35OC), pH (4, 4.5, 5 and 5.5) and the Brix degrees (16, 20, 24 and 28%) during fermentation. Results revealed that the best amount of ethanol was produced at temperature of 30 º C, pH 5, and Brix degree in molasses 16%.
References used
Alkabbashi, A. N., M. Z. Alam and N. Rabbiha. 2011. Media optimization for bioethanol production from sugarcane molasses. ACT-Biotechnol Res Communicat 1(1):20-27
Amutha, R and G. Paramasamy. 2001. Production of ethanol from liquefied cassava starch using co-immobilized cells of Zymomonas mobilis and Saccharomyces diastaticus. J. Biosci. Bioengi., 92: 560-564
Bai, F. W., L. J. Chen, Z. Zhang, W. A. Anderson and M. Moo-Young. 2004. Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions. J. Biotechnol.,110: 287-293
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Producing of biofuel from crops wastes is considered one of the
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showed
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from tree leaves and 14 from food. The isolates AUX system. Were classified
into three Rhodoto
rula species: R. mucilaginosa, R. glutinis and R. minuta with
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breaking down the yeast cells and extracting the carotenoids. It was shown the
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to the yeast biomass and incubating for 24 hrs at 4˚C yielded in higher
quantities when compared with the other five methods. All isolates were able to
produce carotenoides but they varied in their efficiency where the isolate A24
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production which reached 658.23 μg/g dry weight compared with the others.
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galactosidase and diagnose isolates the most efficient in the
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effectiveness of β-galactosidase was estimated using
spectrophotometer at a wavelength of 420 nm.
This research was conducted at the Department of Food Science,
Faculty of Agriculture and National commission for posterity
energy. Twenty kg of apple juice concentrate (70%) were tooked
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orate and prarerd two concentarate (15% and 35%) by using
distilled –sterlization water according to pirson .
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